Cyclic GMP Regulates Nicotine‐Induced Secretion from Cultured Bovine Adrenal Chromaffin Cells: Effects of 8–Bromo–Cyclic GMP, Atrial Natriuretic Peptide, and Nitroprusside (Nitric Oxide)

Abstract

Methacholine, atrial natriuretic peptide (ANP), nitroprusside (nitric oxide), angiotensin II, and bradykinin raised cyclic GMP (cGMP) levels in cultured bovine adrenal chromaffin cells. The role of cGMP in secretion from chromaffin cells was examined using 8-bromo-cGMP. This analogue had no effect on basal secretion or secretion due to angiotensin II, bradykinin, or a high K+ level but potentiated secretion due to low doses of nicotine. At supramaximal doses of nicotine, 8-bromo-cGMP inhibited secretion. These effects of 8-bromo-cGMP were not due to changes in the nicotine-induced rise in cytosolic calcium concentration. A potentiation of secretion due to low doses of nicotine was also found following simultaneous addition of ANP or nitroprusside, a result suggesting that ANP and nitric oxide (endothelium-derived relaxing factor) could be important regulators of secretion from adrenal chromaffin cells.

Resveratrol Inhibits Nicotinic Stimulation-Evoked Catecholamine Release from the Adrenal Medulla

“…Moreover, the presence of endothelial cells has been reported to inhibit the K ＋ -induced or the nicotinic receptor agonist DMPP-induced CA secretion in cultured bovine chromaffin cells (Torres et al, 1994), suggesting that not only nNOS but also eNOS may play roles in modulating adrenal CA secretion. In contrast, it has been shown that L-NAME inhibits ACh-induced CA secretion in bovine chromaffin cells (Uchiyama et al, 1994), and that the NO donor SNP enhances nicotine-induced CA secretion in cultured bovine chromaffin cells (O'Sullivan and Burgoyne, 1990). These findings suggest that NO may facilitate cholinergic agonistinduced CA secretion.…”

Provinol Inhibits Catecholamine Secretion from the Rat Adrenal Medulla

“…Moreover, the presence of endothelial cells has been reported to inhibit the K ＋ -induced or the nicotinic receptor agonist DMPP-induced CA secretion in cultured bovine chromaffin cells (Torres et al, 1994), suggesting that not only nNOS but also eNOS may play roles in modulating adrenal CA secretion. On the contrary, it has been reported that L-NAME inhibits ACh-induced CA secretion in bovine chromaffin cells (Uchiyama et al, 1994) and that the NO donor SNP enhances nicotine-induced CA secretion in cultured bovine chromaffin cells (O'Sullivan and Burgoyne, 1990). These findings suggest that NO may facilitate cholinergic agonist-induced CA secretion.…”

Blockade by Nanomolar Resveratrol of Quantal Catecholamine Release in Chromaffin Cells

“…Thus, blockade by resveratrol of quantal release could be mediated by cGMP, although the role of this nucleotide in regulating catecholamine release is controversial. For instance, O'Sullivan and Burgoyne (1990) found that NO donors augmented catecholamine release. Others found inhibition of secretion (Oset-Gasque et al, 1994;Rodriguez-Pascual et al, 1996).…”