2009
DOI: 10.1002/dvdy.22139
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Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

Abstract: Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it c… Show more

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Cited by 16 publications
(18 citation statements)
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“…This pattern of Notch activity is confirmed by the expression of Nrarp, a direct Notch target in the PSM (Dequeant et al, 2006;Wright et al, 2009), which is severely reduced in the posterior PSM of all Lfng RLFNG/+ embryos examined, with expression restricted to an anterior stripe ( Fig. 2Ca-d).…”
Section: Rlfng Expression Perturbs Cyclic Activity In the Notch Pathwaysupporting
confidence: 54%
See 1 more Smart Citation
“…This pattern of Notch activity is confirmed by the expression of Nrarp, a direct Notch target in the PSM (Dequeant et al, 2006;Wright et al, 2009), which is severely reduced in the posterior PSM of all Lfng RLFNG/+ embryos examined, with expression restricted to an anterior stripe ( Fig. 2Ca-d).…”
Section: Rlfng Expression Perturbs Cyclic Activity In the Notch Pathwaysupporting
confidence: 54%
“…In mouse embryos, targets of the FGF and WNT pathways have been observed to oscillate in the PSM (Dequeant et al, 2006;Wright et al, 2009), and it is clear that both FGF and WNT signaling are important for normal clock function (Aulehla and Pourquie, 2010). To determine whether RLFNG expression perturbs clock-linked genes in other pathways, we examined the expression patterns of Axin2, Spry2 and Snai1.…”
Section: Rlfng Expression Perturbs Cyclic Activity In the Notch Pathwaymentioning
confidence: 99%
“…Proteins encoded by some of these cyclic genes have oscillating levels, as shown by antibody staining (Bessho et al, 2003;Dale et al, 2003), indicating that unstable mRNA and proteins of cyclic genes are expressed and degraded in every cycle of the segmentation clock. Nevertheless, protein levels from very few cyclic genes have been measured, and it remains to be seen how many exhibit meaningful protein oscillations (Wright et al, 2009).…”
Section: Traveling Waves Of Cyclic Gene Expressionmentioning
confidence: 99%
“…Proteins encoded by some of these cyclic genes have oscillating levels, as shown by antibody staining (Bessho et al, 2003;Dale et al, 2003), indicating that unstable mRNA and proteins of cyclic genes are expressed and degraded in every cycle of the segmentation clock. Nevertheless, protein levels from very few cyclic genes have been measured, and it remains to be seen how many exhibit meaningful protein oscillations (Wright et al, 2009).The key observable features of these oscillating patterns are: (1) a traveling wave of gene expression moving anteriorly through the PSM; (2) the slowing and shortening of the wave in the AP direction, and its arrest in the anterior of the PSM at a position that prefigures prospective somite boundaries; and (3) the repetition of the pattern with the formation of each new somite pair. These properties of the traveling wave patterns have been confirmed by time-lapse microscopy in living mouse embryos using luminescent The circadian clock is probably the best-understood biological clock (Hogenesch and Ueda, 2011).…”
mentioning
confidence: 99%
“…Many of these genes encode negative-feedback inhibitors of the pathways, and current models suggest that these feedbacks might be important for oscillations (Goldbeter and Pourquie, 2008;Lewis, 2003). Cyclic genes have also been identified by a candidate gene approach in other species such as chicken (Aulehla and Johnson, 1999;Dale et al, 2006;Jouve et al, 2000;Leimeister et al, 2000;McGrew et al, 1998;Palmeirim et al, 1997;Wright et al, 2009), zebrafish (Henry et al, 2002;Holley et al, 2000;Jiang et al, 2000;Oates and Ho, 2002;Shankaran et al, 2007;Sieger et al, 2004) and Xenopus (Li et al, 2003), indicating an evolutionary conservation of the segmentation clock in vertebrates. In these species, the few known cyclic genes mostly belong to the Notch pathway, including members of the Hairy and enhancer of split (Hes/Her) family of bHLH transcription factors and the Notch glycosyl transferase lunatic fringe (Lfng).…”
Section: Introductionmentioning
confidence: 99%