Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells

Cevallos, Manuel; Yan, Shaoyu; Li, M.; Chai, Hong; Yang, Hui; Yao, Qizhi; Chen, C.

doi:10.1016/j.jss.2004.07.178

Cited by 20 publications

(30 citation statements)

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“…Numerous studies have found that mechanical tension induces expression of SMA. [20][21][22][23] Given those observations, in addition to being a valuable resource for studying smooth muscle cells, SMA-Cre mice are a novel tool for studying the mechanism of the development and maintenance of cranial sutures. …”

Section: )mentioning

confidence: 99%

Human Smooth Muscle α-Actin Promoter Drives Cre Recombinase Expression in the Cranial Suture in Addition to Smooth Muscle Cell

Maruyama

Hatakeyama

Miwa

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Section: )mentioning

confidence: 99%

Human Smooth Muscle α-Actin Promoter Drives Cre Recombinase Expression in the Cranial Suture in Addition to Smooth Muscle Cell

Maruyama

Hatakeyama

Miwa

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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“…This is due to the desirability of replicating physiological conditions as closely as possible in order to promote development of a tissue construct with the appropriate properties. Of the factors listed, cyclic strain and wall shear stress are particularly crucial to mechanically stimulating and engineering a vessel construct with desired physical and mechanical properties (6,11,18,37,43,46,49). The cyclic strain affects the SMCs and helps condition them into developing physical and mechanical properties similar to those found in native vessels.…”

Section: Tissue Engineering: Blood Vesselsmentioning

confidence: 99%

“…Shear stress and cyclic strain have been found to be among the most important factors contributing to enhanced EC and SMC differentiation, phenotypic expression, and mechanical properties (6,11,18,37,43,46,49,59). The wall shear stress is a mechanical stimulus that specifically applies to conditioning endothelial cells because they are in constant contact with the circulating flow of blood within the body.…”

Section: Contemporary Work Engineering Blood Vesselsmentioning

confidence: 99%

“…The cyclic strain stimulates the SMCs to differentiate and produce greater cell density, increased extracellular matrix, maturation, and organization (46, 49). It also increases the ability of endothelial cells to resist blood flow induced shear stress and fluid particulate wall adhesion (6). Since the focus of the thesis is on the implementation of physiologic pressure waves, it is imperative that previously utilized system designs and modifications are understood as well as their potential or expected benefits.…”

Section: Contemporary Work Engineering Blood Vesselsmentioning

confidence: 99%

“…As tissue engineering progressed, and the understanding of the roles hemodynamic factors played in guiding tissue growth increased, studies began integrating cyclic strain, growth factors, and preconditioning to modulate SMC and EC phenotypic and cellular differentiation promotion (6,11,18,37,43,46,49,59). With the variety of cell types and sources available to tissue engineer blood vessels, studies sought to understand how different flow conditions influence specific types of cells.…”

Section: Effects Of Pulsatile Conditionsmentioning

confidence: 99%

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Implementation of Physiologic Pressure Conditions in a Blood Vessel Mimic Bioreactor System

Mr.¹,

Mark²

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Tissue engineering has traditionally been pursued as a therapeutic science intended for restoring or replacing diseased or damaged biologic tissues or organs. Cal Poly's Blood Vessel Mimic Laboratory is developing a novel application of tissue engineering as a tool for the preclinical evaluation of intravascular devices. The blood vessel mimic (BVM) system has been previously used to assess the tissue response to deployed stents, but under non-physiologic conditions. Since then, efforts have been made to improve the vessel and bioreactor's ability to emulate in vivo conditions. The ability to tissue engineer constructs similar to their native tissue counterparts is heavily reliant upon controlling the environment and mechanical stimuli the construct is exposed to. Mimicking physiologic conditions influences cellular growth, proliferation, and differentiation. Two important mechanical stimuli are cyclic strain and wall shear stress.Previous work sought to improve these factors within the BVM bioreactor and resulted in the implementation of pulsatile perfusion and increased fluid viscosity. These previous bioreactor design modifications generated pulsatile pressures of approximately 80 mmHg and a wall shear stress of 6.4 dynes/cm 2 . However, physiologic pressure waveforms were not achieved.Studies in this thesis were carried out to implement an effective means of establishing a more physiologic pressure wave within the bioreactor that is accurate, v consistent, and easily adjustable. As a result of conducting the present studies, modifications to the bioreactor system were made that uphold the overall goals of efficacy and efficiency. The desired pressure wave was created by setting the degree of pump tubing occlusion on the 3-roller peristaltic pump head and using a water column to backpressure the bioreactor chamber. Maintaining a desired backpressure within the system necessitated the development of a new bioreactor chamber with increased extraluminal leak pressure resistance. The opportunity was also used to further improve upon the bioreactor chamber design to allow for 360˚ rotation to reduce cell sedimentation.Modifications to the bioreactor system required quantitative evaluation to assess their impact upon local flow dynamics to the tissue construct. A system model was created and evaluated using computational modeling.Through the work performed in this thesis, pulsatile pressure waves of approximately 120/80 mmHg were successfully established within the bioreactor. The ability to accurately model physiologic pressures will ultimately help yield tissue constructs more similar to native tissues -both healthy and pathological. The newly designed bioreactor chamber and computational model for the system will be helpful tools for implementing or evaluating future bioreactor developments or improvements.While the main objective of the thesis has been completed by creating a system capable of emulating physiologic pressure fluctuations, there still remains room for further improvements in back-pressuring and s...

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Basic fibroblast growth factor uniquely stimulates quiescent vascular smooth muscle cells and induces proliferation and dedifferentiation

Tsuji‐Tamura

Tamura

2022

FEBS Letters

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Blood vessels normally remain stable over the long term. However, in atherosclerosis, vascular cells leave the quiescent state and enter an activated state. Here, we investigated the factors that trigger the breakage of the quiescent state by screening growth factors and cytokines using a vascular smooth muscle cell (SMC) line and an endothelial cell (EC) line. Despite known functions of the tested factors, only basic fibroblast growth factor (bFGF) was identified as a potent trigger of quiescence breakage in SMCs, but not ECs. bFGF disrupted tight SMC‐monolayers and caused morphological changes, proliferation, and dedifferentiation. Human primary SMCs, but not ECs, also showed similar results. Aberrant SMC proliferation is a critical histological event in atherosclerosis. We, thus, provide further insights into the role of bFGF in vascular pathobiology.

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Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells

Cited by 20 publications

References 0 publications

Human Smooth Muscle α-Actin Promoter Drives Cre Recombinase Expression in the Cranial Suture in Addition to Smooth Muscle Cell

Human Smooth Muscle α-Actin Promoter Drives Cre Recombinase Expression in the Cranial Suture in Addition to Smooth Muscle Cell

Implementation of Physiologic Pressure Conditions in a Blood Vessel Mimic Bioreactor System

Basic fibroblast growth factor uniquely stimulates quiescent vascular smooth muscle cells and induces proliferation and dedifferentiation

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