Cyclic stretch augments human rhinovirus induced inflammatory responses in airway epithelial cells

Nikitenko, Sergei; Shariff, Sami; Arnason, Jason; Shelfoon, Chris; Kooi, Cora; Leigh, Richard

doi:10.1186/1710-1492-10-s1-a71

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…A recent meeting abstract noted that rhinovirus infection enhanced cyclic stretch mediated up- Manuscript to be reviewed regulation of pro-inflammatory cytokine expression in human bronchial epithelial cells (Nikitenko et al, 2014). We noticed a significant increase in mRNA expression of genes encoding the pro-inflammatory cytokines IL-8 and IL-1β in the VA10 cells subjected to cyclic stretch (Fig.…”

Section: Treatment With Vitamin D3 and Pba Differentially Affects Strmentioning

confidence: 68%

Peer Review #3 of "Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells (v0.1)"

Zasloff¹

2015

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Mechanical ventilation (MV) of patients can cause damage to bronchoalveolar epithelium, leading to a sterile inflammatory response, infection and in severe cases sepsis. Limited knowledge is available on the effects of MV on the innate immune defense system in the human lung. In this study, we demonstrate that cyclic stretch of the human bronchial epithelial cell lines VA10 and BCi NS 1.1 leads to down-regulation of cathelicidin antimicrobial peptide (CAMP) gene expression. We show that treatment of VA10 cells with vitamin D3 and/or 4-phenyl butyric acid counteracted cyclic stretch mediated downregulation of CAMP mRNA and protein expression (LL-37). Further, we observed an increase in pro-inflammatory responses in the VA10 cell line subjected to cyclic stretch. The mRNA expression of the genes encoding pro-inflammatory cytokines IL-8 and IL-1β was increased after cyclic stretching, where as a decrease in gene expression of chemokines IP-10 and RANTES was observed. Cyclic stretch enhanced oxidative stress in the VA10 cells. The mRNA expression of toll-like receptor (TLR) 3, TLR5 and TLR8 was reduced, while the gene expression of TLR2 was increased in VA10 cells after cyclic stretch. In conclusion, our in vitro results indicate that cyclic stretch may differentially modulate innate immunity by down-regulation of antimicrobial peptide expression and increase in pro-inflammatory responses.

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Section: Treatment With Vitamin D3 and Pba Differentially Affects Strmentioning

confidence: 68%

Peer Review #3 of "Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells (v0.1)"

Zasloff¹

2015

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“…This damage due to MV activated inflammatory responses is further enhanced during infections. A recent meeting abstract noted that rhinovirus infection enhanced cyclic stretch mediated up-regulation of pro-inflammatory cytokine expression in human bronchial epithelial cells ( Nikitenko et al, 2014 ). We noticed a significant increase in mRNA expression of genes encoding the pro-inflammatory cytokines IL-8 and IL-1 β in the VA10 cells subjected to cyclic stretch ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells

Káradóttir

Kulkarni

Gudjonsson

et al. 2015

PeerJ

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Mechanical ventilation (MV) of patients can cause damage to bronchoalveolar epithelium, leading to a sterile inflammatory response, infection and in severe cases sepsis. Limited knowledge is available on the effects of MV on the innate immune defense system in the human lung. In this study, we demonstrate that cyclic stretch of the human bronchial epithelial cell lines VA10 and BCi NS 1.1 leads to down-regulation of cathelicidin antimicrobial peptide (CAMP) gene expression. We show that treatment of VA10 cells with vitamin D3 and/or 4-phenyl butyric acid counteracted cyclic stretch mediated down-regulation of CAMP mRNA and protein expression (LL-37). Further, we observed an increase in pro-inflammatory responses in the VA10 cell line subjected to cyclic stretch. The mRNA expression of the genes encoding pro-inflammatory cytokines IL-8 and IL-1β was increased after cyclic stretching, where as a decrease in gene expression of chemokines IP-10 and RANTES was observed. Cyclic stretch enhanced oxidative stress in the VA10 cells. The mRNA expression of toll-like receptor (TLR) 3, TLR5 and TLR8 was reduced, while the gene expression of TLR2 was increased in VA10 cells after cyclic stretch. In conclusion, our in vitro results indicate that cyclic stretch may differentially modulate innate immunity by down-regulation of antimicrobial peptide expression and increase in pro-inflammatory responses.

show abstract

Cyclic stretch augments human rhinovirus induced inflammatory responses in airway epithelial cells

Cited by 2 publications

References 6 publications

Peer Review #3 of "Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells (v0.1)"

Peer Review #3 of "Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells (v0.1)"

Cyclic mechanical stretch down-regulates cathelicidin antimicrobial peptide expression and activates a pro-inflammatory response in human bronchial epithelial cells

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