Cyclical depressurization degranulates platelets in an agonist-free mechanism of platelet activation

Abstract

Activation of circulating platelets by receptor binding and subsequent coagulation events are defined by a well characterized physiological response. However, the growing prevalence of chronic kidney disease (CKD) and implication of platelet-released factors in worsening cardiovascular outcomes with hemodialysis warrant further investigation into the mechanobiology of platelet degranulation. The significant drops in pressure caused by high friction across the hemodialysis flow circuit present an overlooked platelet stimulant not involving immobilization as a driver for cytoskeletal rearrangement. In this study, platelets from healthy and dialysis (pre- and post-treatment) donors were cyclically depressurized in static suspension to measure changes in physiology by integrin αIIbβ3 activation and surface P-selectin expression. The progressive increase in CD62P with no changes in PAC1 over pressure-cycling duration regardless of uremia signifies that hydrostatic depressurization involves a novel agonist-free mechanism leading to platelet degranulation as a unique case in which CD62P and PAC1 do not interchangeably indicate platelet activation. Subsequent stimulation using ADP further suggests that sustained depressurization regimens desensitize integrin αIIbβ3 activation. Variability in platelet response caused by uremia and CKD are observed by elevated baseline PAC1 in pre-dialysis samples, PAC1 retention after ADP exposure, and maximum CD62P with ADP independent of pressure. Theory for hydrostatic pressure-induced degranulation circumventing integrin-initiated signal transduction is here presented based on the Starling Equation.

Facile preparation of nitrogen/titanium-rich porous organic polymers for specific enrichment ofN-glycopeptides and phosphopeptides

“…The column chart indicates that under expression was seen in the majority of phosphoproteins examined by GO analysis in the blood of uremic patients, including acute-phase response and blood coagulation in biological processes, blood microparticles, platelet alpha granule lumen, and endoplasmic reticulum lumen in cellular components, and serine-type endopeptidase inhibitor activity in molecular functions. 28,32,34–36…”

“…Analytical Methods platelet alpha granule lumen, and endoplasmic reticulum lumen in cellular components, and serine-type endopeptidase inhibitor activity in molecular functions. 28,32,[34][35][36]…”