Cyclicity of mosquito vitellogenic ecdysteroid-mediated signaling is modulated by alternative dimerization of the RXR homologue
            <i>Ultraspiracle</i>

Zhu, Jinsong; Miura, Ken; Chen, Li; Raikhel, Alexander S.

doi:10.1073/pnas.0235695100

Cited by 52 publications

(36 citation statements)

References 30 publications

(36 reference statements)

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“…Purified His-FISC protein was injected into rats (Cocalico Biologicals), and antiserum was affinity purified using immobilized His-FISC. The generation of antigen-purified rabbit polyclonal antibodies against AaUSP and AaEcR, as well as against Aa␤Ftz-F1, has been reported previously (51,52). The monoclonal antibody against DmUSP (anti-DmUSP) was a gift from Fotis C. Kafatos (Imperial College, London, United Kingdom).…”

Section: Methodsmentioning

confidence: 99%

“…The green African monkey kidney CV-1 cell line (American Type Culture Collection) was used for the mammalian two-hybrid assay and transfected as described elsewhere (51). The reporter plasmid 5ϫ UAS-E1B-TATA-Luc containing five tandem repeats of the GAL4 upstream activating sequence was provided by Richard G. Pestell (47).…”

Section: Methodsmentioning

confidence: 99%

“…The emergence of adult mosquitoes was synchronized, and female mosquitoes were collected at 6 h posteclosion (PE), 96 h PE, and 6 h PBM. Chromatin immunoprecipitation (ChIP) assays were then performed as described by Zhu et al (51). Cross-linked extracts were precipitated with antibodies against acetylated histone H4, EcR, ␤Ftz-F1, FISC, or preimmune serum.…”

Section: Methodsmentioning

confidence: 99%

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The Competence Factor βFtz-F1 Potentiates Ecdysone Receptor Activity via Recruiting a p160/SRC Coactivator

Zhu

Chen

Sun

et al. 2006

Molecular and Cellular Biology

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104

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Hormones provide generalized signals that are interpreted in a specific spatial and temporal manner by a developing or reproducing multicellular organism. The ability to respond to hormones is determined by the competence of a cell or a tissue. The ␤Ftz-F1 orphan nuclear receptor acts as a competence factor for the steroid hormone 20-hydroxyecdysone (20E) in Drosophila melanogaster metamorphosis and mosquito reproduction. The molecular nature of the ␤Ftz-F1 action remains unclear. We report that the protein-protein interaction between ␤Ftz-F1 and a p160/SRC coactivator of the ecdysone receptor, FISC, is crucial for the stage-specific expression of the 20E effector genes during mosquito reproduction. This interaction dramatically increases recruitment of FISC to the functional ecdysone receptor in a 20E-dependent manner. The presence of ␤Ftz-F1 facilitates loading of FISC and the ecdysone receptor on the target promoters, leading to enhanced local histone H4 acetylation and robust activation of the target genes. Thus, our results reveal the molecular basis of competence for the stage-specific 20E response.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Competence Factor βFtz-F1 Potentiates Ecdysone Receptor Activity via Recruiting a p160/SRC Coactivator

Zhu

Chen

Sun

et al. 2006

Molecular and Cellular Biology

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104

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“…Drosophila S2 cells [53,54] were cultured in Drosophila Serum-Free Medium (SFM, Invitrogen). The cells were seeded at a density of 2.5 · 10 5 cells per well of 24-well plates one day before transfection.…”

Section: Cell Culture and Transfectionmentioning

confidence: 99%

Characterization of the Drosophila Methoprene ‐tolerant gene product

et al. 2005

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Insect development and reproduction are regulated by two classes of lipid-soluble hormones, the ecdysteroids and juvenile hormones (JHs). The ecdysteroids activate target genes through a heterodimeric receptor complex composing the ecdysone receptor and ultraspiracle (USP) proteins, both of which are members of the nuclear steroid ⁄ thyroid ⁄ retinoid receptor superfamily [1]. During insect development, ecdysteroids induce molting while JH determines the nature of each molt by modulating the ecdysteroid-induced gene expression cascade [2][3][4]. In addition, in adult insects, JH has a wide variety of actions related to reproduction, including oogenesis, migratory behaviour and diapause [2,5,6]. The mode of molecular action of JH, however, is still obscure [7]. JHs are a family of esterified sesquiterpenoids, whose lipid-soluble nature has suggested action directly on the genome through nuclear receptors such as ecdysteroids and the vertebrate steroid ⁄ thyroid ⁄ retinoid hormones [5,8] although actions of JH through the cell membrane are also documented [9,10].Many attempts have been made to identify nuclear JH receptors. Jones and Sharp [11] showed that JH III binds to the Drosophila USP protein, which is a homologue of the vertebrate retinoid X receptor, promoting Juvenile hormones (JHs) of insects are sesquiterpenoids that regulate a great diversity of processes in development and reproduction. As yet the molecular modes of action of JH are poorly understood. The Methoprenetolerant (Met) gene of Drosophila melanogaster has been found to be responsible for resistance to a JH analogue (JHA) insecticide, methoprene. Previous studies on Met have implicated its involvement in JH signaling, although direct evidence is lacking. We have now examined the product of Met (MET) in terms of its binding to JH and ligand-dependent gene regulation. In vitro synthesized MET directly bound to JH III with high affinity (K d ¼ 5.3 ± 1.5 nm, mean ± SD), consistent with the physiological JH concentration. In transient transfection assays using Drosophila S2 cells the yeast GAL4-DNA binding domain fused to MET exerted JH-or JHAdependent activation of a reporter gene. Activation of the reporter gene was highly JH-or JHA-specific with the order of effectiveness: JH III JH II > JH I > methoprene; compounds which are only structurally related to JH or JHA did not induce any activation. Localization of MET in the S2 cells was nuclear irrespective of the presence or absence of JH. These results suggest that MET may function as a JH-dependent transcription factor.

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“…Similarly, in kuruma prawn, RXR expression did not vary across molt stages in hepatopancreas (Asazuma et al 2007). Although RXR is the dimerization partner of EcR in lobsters and other crustaceans, it also forms heterodimers with several other nuclear receptors (Chawla et al 2001, Zhu et al 2003, so more stable RXR expression levels may reflect its dimerization behavior with multiple partners.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression in American Lobster (Homarus americanus) with Epizootic Shell Disease

Tarrant

Franks

Verslycke

2012

Journal of Shellfish Research

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Epizootic shell disease (ESD) has been reported widely in American lobster (Homarus americanus, Milne Edwards) in southern New England. The appearance of irregular, deep lesions-characteristic of ESD-has been associated previously with elevated levels of ecdysteroids and premature molting, but the underlying molecular and physiological changes associated with ESD remain poorly understood. Previously, we identified several genes, including arginine kinase and hemocyanin, that were expressed differentially in lobsters exhibiting signs of ESD (diseased) versus those lobsters exhibiting no signs of ESD (assumed healthy), and quantified their expression. In this study, we extend these findings and measure expression of a suite of 12 genes in tissues from 36 female lobsters of varying disease condition. In addition, molt stage is evaluated as a possible confounding factor in the expression of the selected genes. The expression of several genes changed significantly with disease stage. Arginine kinase expression decreased significantly in thoracic muscle of lobsters with signs of ESD. Ecdysteroid receptor expression was elevated significantly in both muscle and hepatopancreas of lobsters with signs of ESD. CYP45, a cytochrome P450 form that was shown previously to covary with ecdysteroid levels and to be inducible by some xenobiotics, showed significantly increased expression in hepatopancreas of lobsters with signs of ESD. Together, these results demonstrate that the expression of several genes is altered in lobsters showing signs of ESD, even when accounting for variation in molt stage. Given the observed changes in ecdysteroid receptor, arginine kinase, and CYP45 expression, further investigations of the association, if any, between molting, muscular function and xenobiotic metabolism and ESD are warranted.

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Cyclicity of mosquito vitellogenic ecdysteroid-mediated signaling is modulated by alternative dimerization of the RXR homologue Ultraspiracle

Cited by 52 publications

References 30 publications

The Competence Factor βFtz-F1 Potentiates Ecdysone Receptor Activity via Recruiting a p160/SRC Coactivator

The Competence Factor βFtz-F1 Potentiates Ecdysone Receptor Activity via Recruiting a p160/SRC Coactivator

Characterization of the Drosophila Methoprene ‐tolerant gene product

Gene Expression in American Lobster (Homarus americanus) with Epizootic Shell Disease

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