Cyclin and protamine as prognostic molecular marker for testicular sperm extraction in patients with azoospermia

Haraguchi, Takahiro; Ishikawa, Tomomoto; Yamaguchi, Kensei; Fujisawa, Masato

doi:10.1016/j.fertnstert.2008.05.072

Cited by 26 publications

(14 citation statements)

References 11 publications

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“…Additionally, expression levels of these 4 molecular markers were significantly associated with histological findings of testicular biopsy specimens; that is, VASA, ODF1, ODF2, and SMCP expressions in SCO were markedly down‐regulated compared with those in either MA or HS. We also previously reported that the cyclin A1, cyclin B1, cyclin B2, protamine‐1, and protamine‐2 mRNA transcript ratios were significantly decreased in patients with failed sperm retrieval by micro‐TESE compared with those with successful sperm retrieval (Haraguchi et al, 2009). Collectively, these findings strongly suggest that assessment of the expression profile of potential molecular markers playing important roles in spermatogenesis could be used as a useful adjunct to conventional parameters for differentiating successful from unsuccessful groups undergoing micro‐TESE.…”

Section: Discussionmentioning

confidence: 59%

Expression of VASA mRNA in Testis as a Significant Predictor of Sperm Recovery by Microdissection Testicular Sperm Extraction in Patient With Nonobstructive Azoospermia

Ando

Yamaguchi

Chiba

et al. 2012

Journal of Andrology

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It would be of value to evaluate whether expression levels of mRNAs playing important roles in spermatogenesis could be used as predictors of successful testicular sperm recovery by microdissection testicular sperm extraction (TESE) in patients with nonobstructive azoospermia (NOA). This study included 52 patients with NOA who underwent therapeutic sperm retrieval by micro-TESE at our institution. We measured expression levels of VASA, outer dense fiber-1 (ODF1), ODF2, and sperm mitochondria-associated cysteine-rich protein (SMCP) mRNAs in testicular tissue specimens obtained from these patients by quantitative real-time reverse transcription polymerase chain reaction. Expression patterns of these mRNAs were analyzed focusing on the outcomes of sperm retrieval by micro-TESE. There were no significant differences in age, testicular volume, and serum levels of follicle-stimulating hormone, luteinizing hormone, and testosterone between successful and unsuccessful TESE groups; however, the proportion of patients with Sertoli cell only in the successful TESE group was significantly lower than that in the unsuccessful TESE group. Additionally, expression levels of VASA, ODF1, ODF2, and SMCP mRNAs in the successful TESE group were significantly greater than those in the unsuccessful TESE group. Univariate analysis identified histological classification, VASA, ODF1, ODF2, and SMCP expressions as significant predictors of sperm retrieval, of which only VASA mRNA expression appeared to be independently related to micro-TESE outcome on multivariate analysis. These findings suggest that measurement of VASA mRNA in testis could be a useful adjunct to conventional parameters for predicting sperm retrieval by micro-TESE in patients with NOA.

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Section: Discussionmentioning

confidence: 59%

Expression of VASA mRNA in Testis as a Significant Predictor of Sperm Recovery by Microdissection Testicular Sperm Extraction in Patient With Nonobstructive Azoospermia

Ando

Yamaguchi

Chiba

et al. 2012

Journal of Andrology

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“…We showed that several proposed markers; however, no one could foretell about the presence of a spermatogenic focus inside an NOA testis regarding TESE/ICSI processes (13). The invasive examination, such as testicular biopsy, may cause inflammatory changes, hematoma, parenchymal fibrosis, or permanent devascularization of the testes (14, 15) and does not determine whether sperm are present elsewhere in the testis without good molecular markers at present.…”

Section: Discussionmentioning

confidence: 95%

Learning curves of microdissection testicular sperm extraction for nonobstructive azoospermia

Ishikawa

Nose

Yamaguchi

et al. 2010

Fertility and Sterility

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“…Among them, HORMAD2 is known to be essential during meiotic prophase19, while CCNA1 is a meiosis-specific cyclin required for meiosis I in testicular germ epithelium20. The relative abundance of CCNA1 transcript was reported to be lower in patients with severe defects of sperm production20. Network analysis identified another key gene, EZH2.…”

Section: Discussionmentioning

confidence: 99%

Testis Transcriptome Modulation in Klinefelter Patients with Hypospermatogenesis

D’Aurora

Ferlin

Garolla

et al. 2017

Sci Rep

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The main genetic cause of male infertility is represented by the Klinefelter Syndrome (KS), a condition accounting for 3% of all cases of infertility and up to15% of cases of azoospermia. KS is generally characterized by azoospermia; approximately 10% of cases have severe oligozoospermia. Among these, the 30–40% of patients show hypospermatogenesis. The mechanisms leading to adult testis dysfunctions are not completely understood. A microarray transcriptome analysis was performed on testis biopsies obtained from three KS patients with hypospermatogenesis and three control subjects. KS testis showed a differential up- and down-regulation of 303 and 747 transcripts, respectively, as compared to controls. The majority of down-regulated transcripts were involved in spermiogenesis failure and testis morphological defects, whereas up-regulated genes were responsible for testis apoptotic processes. Functional analysis of the transcriptionally altered genes indicated a deregulation in cell death, germ cell function and morphology as well as blood-testis-barrier maintenance and Leydig cells activity. These data support a complex scenario in which spermatogenic impairment is the result of functional and morphological alterations in both germinal and somatic components of KS testis. These findings could represent the basis for evaluating new markers of KS spermatogenesis and potential targets of therapeutic intervention to preserve residual spermatogenesis.

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Cyclin and protamine as prognostic molecular marker for testicular sperm extraction in patients with azoospermia

Cited by 26 publications

References 11 publications

Expression of VASA mRNA in Testis as a Significant Predictor of Sperm Recovery by Microdissection Testicular Sperm Extraction in Patient With Nonobstructive Azoospermia

Expression of VASA mRNA in Testis as a Significant Predictor of Sperm Recovery by Microdissection Testicular Sperm Extraction in Patient With Nonobstructive Azoospermia

Learning curves of microdissection testicular sperm extraction for nonobstructive azoospermia

Testis Transcriptome Modulation in Klinefelter Patients with Hypospermatogenesis

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