Cyclin D1 Protein Tissue Detection in Laryngeal Cancer

Abstract

Cyclin D1 (CCND1) is a set of periodic regulatory proteins that is believed to govern cell cycle transit from G1 into S phase. Overexpression of CCND1 leads to abnormal cellular proliferation which underlies processes of tumorigenesis; CCND1 can thus function as a cooperative oncogene in cell transformation. In the present study we investigate the immunohistochemical expression of CCND1 in a well-documented series of 58 laryngeal squamous cell carcinomas (LSCC) and search for statistical associations between CCND1 index and various clinicopathological parameters including several immunomarkers’ expression as well as patients’ disease-free survival. Tissue sections from archival paraffin blocks were stained using the avidin-biotin-peroxidase complex method; the H-295 rabbit polyclonal antibody was applied at dilution of 1:150. The percentage of CCND1 immunoreactive tumor cells for each tumor was counted by an image analysis system. CCND1 staining was confined to cell nuclei and, in the examined samples, ranged from undetectable (i.e. 0% of tumor cells, n = 6) to the majority of tumor cells (i.e. 89% of tumor cells) with mean value: 15.73%. In tumor adjacent, non invasive lesions, strong CCND1 staining was noticed in areas with cellular atypia. In cases with nodal metastases, no change in CCND1 expression in the nodal metastases compared with the primary tumors was observed. p53 protein accumulation in malignant cells was positively linked with CCND1 index (Mann-Whitney U: 205.5, p = 0.034). CCND1 expression appears to be an early event in processes of tumorigenesis and tumor progression in some LSCC. Apart from p53 protein accumulation, CCND1 immunohistochemical expression does not seem to correlate with nodal metastasis, disease recurrence or any other clinicopathological prognostic indicator.

An immunohistochemical study of cyclin D1 protein expression in laryngeal squamous cell carcinoma

“…The difference in the results may be connected with the small number of patients evaluated in previous studies and different lymph node involvement distribution. Similar results were reported by Segas et al [17]; they also failed to find any correlation of clinicopathological features and cyclin D1 expression.…”

“…This criterion, even if arbitrary, has also been chosen by other researchers [15,18,19]. In some studies other scorings of cyclin D1 expression were used and thus comparison of the results is difficult [17,24]. In our study there was no statistically significant relationship between cyclin D1 expression and demographic and clinicopathological variables.…”

Chromosome 11 aneusomy in esophageal cancers and precancerous lesions- an early event in neoplastic transformation: An interphase fluorescencein situhybridization study from south India

“…Chromosome 11 has a number of oncogenes and tumor suppressors including Cyclin D1, which is overexpressed in a wide variety of human neoplasms [18,19] . Reports suggest that overexpression of cyclin D1 is not due to gene amplification but due to chromosome number changes [5] .…”

The importance of quantifying markers of oncogenesis in tissue slices of patients with squamous cell carcinoma of the larynx and chronic laryngitis

“…, р=0,050, појединачно). Опречни су резултати добијени за патолошке параметре истраживања у до сада познатој литератури(268,(285)(286)(287)(337)(338)(339)(340)(341)(342)). Већи број истраживања је показао да немаповезаности експресије сyclin D1, р53 и Ki67 са TNM и хистолошким градусом карцинома ларинкса (266-331).…”