CYCLIN H;1 Regulates Drought Stress Responses and Blue Light-Induced Stomatal Opening by Inhibiting Reactive Oxygen Species Accumulation in Arabidopsis

Zhou, Xiaofeng; Jin, Yin Hua; Yoo, Chan Yul; Lin, Xiangui; Kim, Woe‐Yeon; Yun, Dae‐Jin; Bressan, Ray A.; Hasegawa, Paul M.; Jin, Jing Bo

doi:10.1104/pp.113.215798

Cited by 46 publications

(30 citation statements)

References 56 publications

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“…3b,c). Various stimuli can lead to stomatal closure, but stomatal opening is predominately evoked by means of wavelength-responsive mechanisms 27 . The regulatory mechanism of stomatal opening by blue light has been well studied in a number of C 3 plants 28,29 , but its role is less clear in CAM plants 30 light-induced switch from C 3 to CAM in Clusia minor 31 .…”

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confidence: 99%

Transcript, protein and metabolite temporal dynamics in the CAM plant Agave

et al. 2016

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Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C 3 and C 4 photosynthesis) day/ night pattern of stomatal closure/opening to shift CO 2 uptake to the night, when evapotranspiration rates are low. A systems-level understanding of temporal molecular and metabolic controls is needed to define the cellular behaviour underpinning CAM. Here, we report high-resolution temporal behaviours of transcript, protein and metabolite abundances across a CAM diel cycle and, where applicable, compare the observations to the well-established C 3 model plant Arabidopsis. A mechanistic finding that emerged is that CAM operates with a diel redox poise that is shifted relative to that in Arabidopsis. Moreover, we identify widespread rescheduled expression of genes associated with signal transduction mechanisms that regulate stomatal opening/closing. Controlled production and degradation of transcripts and proteins represents a timing mechanism by which to regulate cellular function, yet knowledge of how this molecular timekeeping regulates CAM is unknown. Here, we provide new insights into complex post-transcriptional and -translational hierarchies that govern CAM in Agave. These data sets provide a resource to inform efforts to engineer more efficient CAM traits into economically valuable C 3 crops.T he water-use efficiency of Agave spp. hinges on crassulacean acid metabolism (CAM), a specialized mode of photosynthesis that evolved from ancestral C 3 photosynthesis in response to water and CO 2 limitation 1 and is found in ∼6.5% of higher plants. Whereas C 3 photosynthesis produces a three-carbon (3-C) molecule for carbon fixation during the day, CAM generates a four-carbon organic acid from carbon fixation at night. In CAM, this nocturnal carboxylation reaction is catalysed by phosphoenolpyruvate carboxylase (PEPC), and the 3-C substrate phosphoenolpyruvate (PEP) is supplied by the glycolytic breakdown of carbohydrate formed during the previous day. The nocturnally accumulated malic acid is stored overnight in a central vacuole, and during the subsequent day malate is decarboxylated to release CO 2 at an elevated concentration for Rubisco in the chloroplast. The diel separation of carboxylases in CAM is accompanied by an inverse (compared with C 3 and C 4 photosynthesis-performing species) day/night pattern of stomatal closure/ opening that results in improved water-use efficiency (CO 2 fixed per unit water lost) that is up to sixfold higher than that of C 3 photosynthesis plants and up to threefold higher than that of C 4 photosynthesis plants under comparable conditions 2 .The frequent emergence of CAM from C 3 photosynthesis throughout evolutionary history implies that all of the enzymes required for CAM are homologues of ancestral forms found in C 3 species 1,3 . As such, the CAM pathway has been identified as a target for synthetic biology because it offers the potential to engin...

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confidence: 99%

Transcript, protein and metabolite temporal dynamics in the CAM plant Agave

et al. 2016

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“…The above mentioned two WRKY family transcription factors might be also involved in PeGs biosynthesis in C. salsa. Interestingly, dehydration related ESTs (CISTH067, CISTH064) were found in the SSH library, which may suggest that the H 2 O 2 treatment was related to dehydration responsive genes expression in C. salsa as observed in other plants (Schmidt et al 2013, Zhou et al 2013. One gibberellin (GA) signalling related gene, gibberellin receptor gid1b-like (CISTH092), was identified in the SSH library implying the connection of GA signalling and H 2 O 2 treatment in C. salsa, which was also reported in barley (Bahin et al 2011).…”

Section: Discussionmentioning

confidence: 87%

Identification of hydrogen peroxide responsive ESTs involved in phenylethanoid glycoside biosynthesis in Cistanche salsa cell culture

Chen¹,

Yan²,

Guo³

2015

Biologia plant.

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Hydrogen peroxide is an effective abiotic elicitor that can induce secondary metabolite biosynthesis in plants. We show that in cell suspension culture of a salt-tolerant medicinal plant Cistanche salsa, the production of bioactive components phenylethanoid glycosides (PeGs) was increased after an H 2 O 2 treatment. To identify genes related to PeGs biosynthesis affected by H 2 O 2 , we constructed a suppression subtractive hybridization library of H 2 O 2 responsive genes using a C. salsa cell line and identified 105 expressed sequence tags (ESTs) and 85 genes. EST library functional annotation and gene ontology analyses showed genes related to various stress responses, biosynthesis of secondary metabolites, and transcriptional regulation. Among them, we identified two genes related to the PeGs biosynthesis pathway (4-coumarate coenzyme A ligase and cinnamate 4-hydroxylase), and two WRKY type transcription factors. The expressions of selected genes after the H 2 O 2 treatment were analyzed by RT-qPCR. An early increased transcription of PeGs biosynthesis pathway genes after the treatment revealed that H 2 O 2 induced PeGs biosynthesis via up-regulation of its key genes.Additional key words: cinnamate-4-hydroxylase, 4-coumarate coenzyme A ligase, suppression subtractive hybridization.

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“…CYCH;1 induces the expression of redox homeostasis genes, such as LIPOXYGENASE3 (LOX3), LOX4, ARABIDOPSIS GLUTATHIONE PEROXIDASE 7 (ATGPX7), EARLY LIGHT-INDUCIBLE PROTEIN1 (ELIP1), and ELIP2, and increased hydrogen peroxide production in guard cells. The downregulation of CYCH;1 impaired the blue light-induced stomatal opening but did not affect the guard cell development or ABA-mediated stomata closure (Zhou et al 2013 ). This clearly suggested that there exist distinct mechanisms for opening stomata under optimal light condition and for closing under stress condition.…”

Section: Stomata Movementmentioning

confidence: 99%

Redox-Regulated Mechanisms: Implications for Enhancing Plant Stress Tolerance and Crop Yield

Srivastava

Suprasanna

2015

Elucidation of Abiotic Stress Signaling in Plants

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In the present scenario of continuously rising world population and climate change, a major thrust in the fi eld of plant biology is to strive for sustainable agriculture. This necessitates a complete understanding of the mechanism underlying plant tolerance against a multitude of stress factors which limit crop productivity. Among other regulators, redox-state homeostasis has recently emerged as the central or core regulator behind stress-induced signaling. At any given point, redox state is defi ned as the integrated ratio of reduced and oxidized forms of all the redox couples present inside the cell and is governed by the level of reactive oxygen species (ROS) and activities of ROS-producing and ROS-scavenging enzymes. The redox-state homeostasis is a highly dynamic and variable component and is strictly dependent upon the developmental stage as well as the external environment. Considering the signifi cance of redox state in regulating multiple plant processes, a large number of redox-associated genes/transcription factors are currently being characterized using functional genomics-based approach. The present chapter deals with the basic aspect of generating redox signal and then describes selected mechanisms, which are responsible for the viewpoint of plant tolerance and crop yield. The future research directions are also discussed so as to ensure the use of these genetic components in crop improvement program.

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CYCLIN H;1 Regulates Drought Stress Responses and Blue Light-Induced Stomatal Opening by Inhibiting Reactive Oxygen Species Accumulation in Arabidopsis

Cited by 46 publications

References 56 publications

Transcript, protein and metabolite temporal dynamics in the CAM plant Agave

Transcript, protein and metabolite temporal dynamics in the CAM plant Agave

Identification of hydrogen peroxide responsive ESTs involved in phenylethanoid glycoside biosynthesis in Cistanche salsa cell culture

Redox-Regulated Mechanisms: Implications for Enhancing Plant Stress Tolerance and Crop Yield

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