CyclinA1 Promoter Methylation in Self-Sampling Test

Oranratanaphan, Shina; Kengsakul, Malika; Triratanachat, Surang; Kitkumthorn, Nakarin; Mutirangura, Apiwat; Termrungruanglert, Wichai

doi:10.31557/apjcp.2020.21.10.2913

Cited by 3 publications

(5 citation statements)

References 18 publications

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“…The present study revealed that TRH methylation had the highest sensitivity and specificity for detecting CIN2+ lesions compared to hr-HPVs alone. Other investigations, which used a panel of DNA methylation markers, such as CADM1, MAL, miR124, CCNA1, PAX1, SOC1, and HPVL1, also exhibited lower sensitivity than TRH methylation [ 33 , 34 , 42 , 43 , 51 , 52 , 53 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

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Efficiency of CIN2+ Detection by Thyrotropin-Releasing Hormone (TRH) Site-Specific Methylation

Chaiwongkot,

Buranapraditkun,

Oranratanaphan

et al. 2023

Viruses

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Cervical cancer screening typically involves a Pap smear combined with high-risk human papillomavirus (hr-HPV) detection. Women with hr-HPV positivity but normal cytology, as well as those with precancerous abnormal cytology, such as low-grade squamous intraepithelial lesions (LSIL) and high-grade SIL (HSIL), are referred for colposcopy and histology examination to identify abnormal lesions, such as cervical intraepithelial neoplasia (CIN) and cervical cancer. However, in order to enhance the accuracy of detection, bioinformatics analysis of a microarray database was performed, which identified cg01009664, a methylation marker of the thyrotropin-releasing hormone (TRH). Consequently, a real-time PCR assay was developed to distinguish CIN2+ (CIN2, CIN3, and cervical cancer) from CIN2- (CIN1 and normal cervical epithelia). The real-time PCR assay utilized specific primers targeting methylated cg01009664 sites, whereas an unmethylated reaction was used to check the DNA quality. A cut-off value for the methylated reaction of Ct < 33 was established, resulting in improved precision in identifying CIN2+. In the first cohort group, the assay demonstrated a sensitivity of 93.7% and a specificity of 98.6%. In the cytology samples identified as atypical squamous cells of undetermined significance (ASC-US) and LSIL, the sensitivity and specificity for detecting CIN2+ were 95.0% and 98.9%, respectively. However, when self-collected samples from women with confirmed histology were tested, the sensitivity for CIN2+ detection dropped to 49.15%, while maintaining a specificity of 100%. Notably, the use of clinician-collected samples increased the sensitivity of TRH methylation testing. TRH methylation analysis can effectively identify women who require referral for colposcopy examinations, aiding in the detection of CIN2+.

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Section: Discussionmentioning

confidence: 99%

“…Cohort 3 and cohort 4 also consisted of leftover specimens from a previous study [ 43 ]. Cohort 3 comprised patient self-collected samples for a total of 275.…”

Section: Methodsmentioning

confidence: 99%

Efficiency of CIN2+ Detection by Thyrotropin-Releasing Hormone (TRH) Site-Specific Methylation

Chaiwongkot,

Buranapraditkun,

Oranratanaphan

et al. 2023

Viruses

Self Cite

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“… 32 Several studies on HPV self‐collection testing have shown increased screening coverage in remote areas or those with high levels of nonattendance. 28 , 32 However, HPV self‐collection should be implemented with careful consideration based on local context and with continuous evaluation. 28 …”

Section: Paradigm Shifts In Cervical Cancer Screeningmentioning

confidence: 99%

“…30 In addition, HPV-based testing can be performed using selfcollection, which has the potential to overcome the barriers encountered with clinician-based screening methods. 32 Several studies on HPV self-collection testing have shown increased screening coverage in remote areas or those with high levels of nonattendance. 28,32 However, HPV self-collection should be implemented with careful consideration based on local context and with continuous evaluation.…”

Section: Par Ad I G M S Hif Ts In Cervi C Al C An Cer Screeningmentioning

confidence: 99%

“…32 Several studies on HPV self-collection testing have shown increased screening coverage in remote areas or those with high levels of nonattendance. 28,32 However, HPV self-collection should be implemented with careful consideration based on local context and with continuous evaluation. 28 In 2020, the American Cancer Society recommended cervical cancer screening with HPV test alone every 5 years for every woman with a cervix from the age of 25-65 years.…”

Section: Par Ad I G M S Hif Ts In Cervi C Al C An Cer Screeningmentioning

confidence: 99%

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Worldwide initiatives to eliminate cervical cancer

Wilailak

Kengsakul

Kehoe

2021

Intl J Gynecology & Obste

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In 2020, more than 600 000 women were diagnosed with cervical cancer and 342 000 women died worldwide. Without comprehensive control, rates of cervical cancer incidence and mortality are expected to worsen. In 2020, the World Health Organization adopted the global strategy to eliminate cervical cancer to the threshold of four cases per 100 000 women within the 21st century, using a triple pillar intervention strategy comprising 90% of girls fully vaccinated by the age of 15 years, 70% of women screened by the age of 35 years and again by 45 years, and 90% of women with precancer treated and 90% of women with invasive cancer managed. In countries with high cervical cancer incidence, a tremendous effort will be needed to overcome the challenges. This article discusses the efforts in place to accelerate achievement of this ambitious goal.

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The current state of DNA methylation biomarkers in self-collected liquid biopsies for the early detection of cervical cancer: a literature review

Sumiec,

Yim,

Mohy-Eldin

et al. 2024

Infect Agents Cancer

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Cervical cancer (CC) is a preventable disease and treatable cancer. Most of the new cases and deaths from CC occur in Low- and Middle-Income Countries (LMICs) due to cultural and systematic barriers leading to low CC screening uptake. In recent years, self-sampling has been proposed as a method to increase CC screening uptake and is slowly being implemented into screening programmes worldwide. Simultaneously, DNA methylation has been proposed as a novel biomarker that could be used for the triage of self-collected samples that test positive for high-risk types of Human Papillomavirus (HPV). In this paper, we conducted a literature review of studies assessing the efficacy of DNA methylation markers to detect Cervical Intraepithelial Neoplasia (CIN) in self-collected cervicovaginal swabs or urine (2019–2024). Our review showed that, of the available data, DNA methylation together with self-sampling could perform as well as cytology in the detection of CIN as well as improve uptake of CC screening and reduce loss to follow up, especially in LMICs. However, more data is still needed to understand which methylation tests are most efficacious. Future studies should assess the full potential of DNA methylation and self-sampling in large, diverse screening cohorts. Supplementary Information The online version contains supplementary material available at 10.1186/s13027-024-00623-1.

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CyclinA1 Promoter Methylation in Self-Sampling Test

Cited by 3 publications

References 18 publications

Efficiency of CIN2+ Detection by Thyrotropin-Releasing Hormone (TRH) Site-Specific Methylation

Efficiency of CIN2+ Detection by Thyrotropin-Releasing Hormone (TRH) Site-Specific Methylation

Worldwide initiatives to eliminate cervical cancer

The current state of DNA methylation biomarkers in self-collected liquid biopsies for the early detection of cervical cancer: a literature review

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