Cycloartane triterpenoids and biological activities from the propolis of the stingless bee Lisotrigona furva

Abstract

Chromatographic separation of ethyl acetate extract of the stingless bee Lisotrigona furva propolis, that collected in Khanh Hoa province, led to isolation of five cycloartane‐type triterpenes including cycloartenone (1), cycloartenol (2), (24E)‐3β‐hydroxycycloart‐24‐en‐26‐al (3), mangiferonic acid (4) and mangiferolic acid (5). Mango plant (Mangifera indica) was suggested to be a resin source of the propolis. The propolis EtOH extract showed good antimicrobial activity on Gram (+) strain E. feacalis and fungus C. albicans. Among the isolated compounds, compound 3 displayed selective activity against three Gram (+) strains and C. albicans over Gram (‐) strains with all MIC values of 64 μg/mL. However, the propolis EtOH extract was not active against LU‐1 and MCF‐7 cancer cell lines. Only compound 5 exhibited moderate activity on LU‐1 cancer cell line with IC50 of 13.33 μg/mL.

Antimicrobial, Cytotoxic, and α-Glucosidase Inhibitory Activities of Ethanol Extract and Chemical Constituents Isolated from Homotrigona apicalis Propolis—In Vitro and Molecular Docking Studies

“…The 1 H, 13 C, HSQC, and HMBC NMR spectra were obtained using the Bruker AVANCE III HD 500 MHz and Bruker AVANCE NEO 600 MHz spectrometers (Bruker, Billerica, MA, USA); the chemical shift (δ) was expressed in ppm using TMS as an internal standard. The ESI-MS spectra were obtained using an Agilent 1260 series single quadrupole LC/MS system (Agilent, Santa Clara, CA, USA).…”

“…Life 2023, 13,1682 Data Availability Statement: The data presented in this study are available upon request from the corresponding author. Data is contained within the article and Supplementary Material.…”

“…Recently, the scientists’ attention to stingless bee propolis has been growing, especially in Australia and the Indo-Malay peninsula due to the diverse species of stingless bees in these regions [ 3 ]. Previous chemical studies of stingless bee propolis in Australia and Southeast Asian countries led to the isolation of diverse chemical compounds, including triterpenes, flavonoids, coumarins, and xanthones [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ].…”