Cyclodextrin/chlorophyll a complexes as supramolecular photosensitizers

Dentuto, Pasquale L.; Catucci, Lucia; Cosma, Pinalysa; Fini, Paola; Agostiano, Angela; Hackbarth, Steffen; Rancan, Fiorenza; Roeder, Beate

doi:10.1016/j.bioelechem.2006.03.026

Cited by 27 publications

(27 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spectra are characterized by an intense Soret band at about 422 nm in the blue region of the visible spectrum and a Qy (0,0) band in the red region at about 663 nm. Similar to that observed in water [11] the peak in the red region shows an enlargement which indicates the presence of pigment aggregates [16,17]. In these spectra the aggregated species absorbing at 713 nm observed in aqueous solutions of HP-β-CD and HP-γ-CD and already evidenced in the water-rich region of binary water/organic solvent mixtures [18] is not evident.…”

Section: Solubilization Of Chl A/cd In Rpmi Mediumsupporting

confidence: 69%

“…The aggregation status of Chl a and the ability of CDs to solubilize the pigment in the cellular medium is different from that observed in water [8][9][10][11]. In particular TRIMEB, the permethylated cyclodextrin having the best ability to solubilize Chl a in water as monomer, is the one which works worst in the RPMI 1640 medium.…”

Section: Resultsmentioning

confidence: 99%

“…4A right). The reduction in the cell survival reported in the previous study [11] might therefore be due to the intrinsic toxicity of the system.…”

Section: Phototoxicitymentioning

confidence: 90%

“…Preliminary experiments aimed to test in vitro the efficiency of these systems in PDT have been recently performed and have evidenced that Chl a/CDs seems to act as sensitizer toward Jurkat cells [11].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Phototoxicity and cytotoxicity of chlorophyll a/cyclodextrins complexes on Jurkat cells

Cosma

Fini

Rochira

et al. 2008

Bioelectrochemistry

View full text Add to dashboard Cite

Section: Solubilization Of Chl A/cd In Rpmi Mediumsupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

“…4A right). The reduction in the cell survival reported in the previous study [11] might therefore be due to the intrinsic toxicity of the system.…”

Section: Phototoxicitymentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phototoxicity and cytotoxicity of chlorophyll a/cyclodextrins complexes on Jurkat cells

Cosma

Fini

Rochira

et al. 2008

Bioelectrochemistry

View full text Add to dashboard Cite

“…The preparation of β-CD-hemin (CDH) was following the previous report Dentuto et al 2007) with minor modification. Hemin and β-CD were mixed at a suitable molar ratio by grinding for 60 min after the addition of de-ionized water.…”

Section: Chemicalsmentioning

confidence: 99%

Induction of heme oxygenase-1 with β-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa

Zhang

Cui

et al. 2011

Plant Soil

View full text Add to dashboard Cite

Using pharmacological and biochemical approaches, the functional roles of heme oxygenase-1 (HO-1) induction in cadmium (Cd)-stressed alfalfa (Medicago sativa) seedling roots caused by a metalorganic complex (β-cyclodextrin-hemin, β-CDhemin, CDH) and the well-known HO-1 inducer hemin, were investigated and compared. Cd-stressed seedling roots exhibited severe oxidative damage, whereas up-regulation of HO-1 by CDH and hemin pretreatment prevented this toxic response, as evidenced by the decrease of lipid peroxidation as well as the alleviation of growth inhibition and Cd accumulation. Reestablishment of glutathione (GSH) and reactive oxygen species (ROS) homeostasis were observed. Upon Cd stress, 0.01 μM CDH and 10 μM hemin pretreatment alleviated lipid peroxidation to almost the same extent and the CDH was highly potent. The modulation of the transcripts and total activities of ascorbate peroxidase (APX) and superoxide dismutase (SOD) were regulated by CDH and hemin, respectively. We also noticed that the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPP) significantly reversed the CDH-driven cytoprotective roles. Additionally, β-CD was found to be ineffective in the alleviation of lipid peroxidation. Taken together, the data suggested that HO-1 induction mediated by CDH provides critical protection against Cd-induced oxidative damage and toxicity partly by lowering the Cd accumulation.

show abstract

β-Cyclodextrin–hemin complex-induced lateral root formation in tomato: involvement of nitric oxide and heme oxygenase 1

Zhu

Ren

et al. 2014

Plant Cell Rep

View full text Add to dashboard Cite

β-Cyclodextrin-hemin complex-induced tomato lateral root formation was associated with nitric oxide and heme oxygenase 1 by modulating cell cycle regulatory genes. β-Cyclodextrin-hemin complex (β-CDH), a complex by combining β-cyclodextrin (β-CD) with hemin, a heme oxygenase 1 (HO1) inducer, was a trigger of cucumber adventitious root formation by enhancing HO1 gene expression. In this report, our results identified the previously unknown function of β-CDH in plants: the inducer of tomato lateral root (LR) formation. β-CDH-triggered LR formation is hemin-specific, since β-CD failed to induce LR development. Because nitric oxide (NO) is involved in LR formation, the correlation of β-CDH with NO and HO1 was investigated. Our analysis suggested that β-CDH induced an increase in endogenous NO production, followed by up-regulation of tomato HO1 gene and LR formation, all of which were mimicked by hemin and two NO-releasing compounds (SNP and GSNO). The induction of HO1 gene expression and LR formation triggered by β-CDH or hemin were significantly blocked by an inhibitor of HO1. Further results revealed that both β-CDH- and SNP-stimulated HO1 gene expression and thereafter LR formation were sensitive to the removal of NO with a potent NO scavenger, and the responses of SNP were significantly blocked by an inhibitor of HO1. Molecular evidence illustrated that representative cell cycle regulatory genes, including SlCDKA1, SlCYCA3;1, SlCYCA2;1, and SlCYCD3;1, were significantly up-regulated by β-CDH and SNP, but obviously blocked when seedlings were co-treated with the scavenger of NO or the inhibitor of HO1. In summary, our physiological and molecular evidence demonstrated that both NO and HO1 were involved in the β-CDH-induced LR formation with, at least partially, HO1 acting downstream of NO signaling.

show abstract

Cyclodextrin/chlorophyll a complexes as supramolecular photosensitizers

Cited by 27 publications

References 17 publications

Phototoxicity and cytotoxicity of chlorophyll a/cyclodextrins complexes on Jurkat cells

Phototoxicity and cytotoxicity of chlorophyll a/cyclodextrins complexes on Jurkat cells

Induction of heme oxygenase-1 with β-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa

β-Cyclodextrin–hemin complex-induced lateral root formation in tomato: involvement of nitric oxide and heme oxygenase 1

Contact Info

Product

Resources

About