Cyclodextrin polysulphates repress IL-1 and promote the accumulation of chondrocyte extracellular matrix

Verdonk, Peter; Wang, J; Groeneboer, Sara; Broddelez, C; Elewaut, Dirk; Veys, Eric; Verbruggen, G.

doi:10.1016/j.joca.2005.02.014

Cited by 8 publications

(13 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This cytokines also blunt chondrocyte compensatory synthesis pathways required to restore the integrity of the degraded ECM [5,6,7] . Thus cytokines activate the enzymatic degradation of cartilage matrix and are not counterbalanced by adequate synthesis of inhibitors.…”

Section: Introductionmentioning

confidence: 99%

<title>Effect of LED irradiation on the expression of MMP-3 and MMP-13 in SW1353 cells in vitro</title>

et al. 2007

View full text Add to dashboard Cite

Matrix Metalloproteinase (MMP) plays an active role in remodeling cartilage in osteoarthritic cartilage. To find an effective method of prevention of osteoclasia, this in vitro study focuses on the expression of MMP-3 and MMP-13 in the SW1353 cells by LED irradiation. The human chondrosarcoma cell line SW1353 were stimulated with the proinflammatory cytokine IL-1beta or tumor necrosis factor-alpha (TNF-alpha), and were received the irradiation of LED (632nm, 4mW/cm2). The cell count was assessed over a 96-hour period by using Trypan blue dye exclusion assay, and the cell activity was evaluated with a Cell Counting Kit-8 Assays. The subsequent expression of MMP-3 and MMP-13 was quantified. Results of this experiment showed that the cultural cell activity was decreased, and the expression of MMP-3 and MMP-13 was increased by being stimulated with IL-1beta or TNF-alpha. After received LED irradiation, the death rate of cultural cell was increased and the expression of MMP-3 and MMP-13 was decreased significantly. The present study concluded that particular LED irradiation stimulates SW1353 cell proliferation activity and inhibit the MMP-3 and MMP-13 enzymatic activity. These findings might be clinically relevant, indicating that the low power laser irradiation treatment is likely to achieve the repair of articular cartilage in clinic.

show abstract

Section: Introductionmentioning

confidence: 99%

<title>Effect of LED irradiation on the expression of MMP-3 and MMP-13 in SW1353 cells in vitro</title>

et al. 2007

View full text Add to dashboard Cite

show abstract

“…In vitro , all three α-, β- and γ-CDPS equally and significantly improved the synthesis and accumulation by chondrocytes of CAM macromolecules e.g. aggrecan, Type II collagen and fibronectin [17]. It was shown that this enhanced repair function resulted from a down-modulation of the autocrine catabolic IL-1 pathway: β-CDPS inhibited the synthesis of IL-6 in both normal and OA chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…It was shown that this enhanced repair function resulted from a down-modulation of the autocrine catabolic IL-1 pathway: β-CDPS inhibited the synthesis of IL-6 in both normal and OA chondrocytes. Although the experiments were not performed in the presence of IL-1-blocking antibodies, it is supposed that β-cyclodextrin affected the IL-1 loop and did not directly impede the production of IL-6 [17]. While in osteoarthritic chondrocytes both autocrine anabolic (IGF-1) and catabolic (IL-1) pathways are up-regulated, the selective inhibition of IL-1 resulted in a dramatic improvement of the repair function.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a novel polysulphated polysaccharide, cyclodextrin polysulphate (CDPS) was reported to induce a down-regulation of intracellular IL-1α and -β and to cause a concomitant increase in the synthesis of aggrecan, collagen Type II and fibronectin in the cell-associated matrix (CAM) of human chondrocytes cultured in alginate beads [17]. This CDPS also depressed the IL-6 release of OA chondrocytes by 60% compared with untreated OA cartilage cells and equalled the levels secreted by normal cells [17].…”

Section: Introductionmentioning

confidence: 99%

“…This CDPS also depressed the IL-6 release of OA chondrocytes by 60% compared with untreated OA cartilage cells and equalled the levels secreted by normal cells [17]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimized alkylated cyclodextrin polysulphates with reduced risks on thromboembolic accidents improve osteoarthritic chondrocyte metabolism

et al. 2011

Self Cite

View full text Add to dashboard Cite

Objectives. To compare the ability of different cyclodextrin polysulphate (CDPS) derivatives to affect human articular cartilage cell metabolism in vitro.Methods. OA chondrocytes were cultured in alginate and exposed to 5 µg/ml of 2,3,6-tri-O-methyl-β-cyclodextrin (ME-CD), 2,3-di-O-methyl-6-sulphate-β-cyclodextrin (ME-CD-6-S), 2,6-di-O-methyl-3-sulphate-β-cyclodextrin (ME-CD-3-S), (2-carboxyethyl)-β-CDPS (CE-CDPS), (2-hydroxypropyl)-β-CDPS (HP-CDPS), 6-monoamino-6-monodeoxy-β-CDPS (MA-CDPS) or β-CDPS for 5 days. Effects on IL-1-driven chondrocyte extracellular matrix (ECM) metabolism were assayed by analysis of the accumulation of aggrecan in the interterritorial matrix, IL-6 secretion and qPCR. MA-CDPS, HP-CDPS, CE-CDPS and CDPS were analysed for their in vitro effect on coagulation and their ability to activate platelets in an in vitro assay to detect possible cross-reactivity with heparin-induced thrombocytopenia (HIT) antibodies.Results. The monosulphated cyclodextrins ME-CD-6-S and -3-S failed to affect aggrecan synthesis and IL-6 secretion by the OA chondrocytes. Polysulphated cyclodextrins MA-CDPS, HP-CDPS, CE-CDPS and CDPS at 5 µg/ml concentrations, on the other hand, significantly induced aggrecan production and repressed IL-6 release by the chondrocytes in culture. aPTT and PT for all derivatives were lengthened for polysaccharide concentrations >50 µg/ml. Five micrograms per millilitre of β-CDPS concentrations that significantly modulated ECM ground substance production in vitro did not affect aPTT or PT. Furthermore, CE-CDPS, in contrast to MA-CDPS, HP-CDPS and CDPS, did not significantly activate platelets, suggesting a minimal potential to induce HIT thromboembolic accidents in vivo.Conclusions. CE-CDPS is a new, structurally adjusted, sulphated β-cyclodextrin derivative with preserved chondroprotective capacity and a promising safety profile.

show abstract

Anti‐Inflammatory Nanotherapies Based on Bioactive Cyclodextrin Materials

Li,

Chen,

et al. 2023

Advanced NanoBiomed Research

View full text Add to dashboard Cite

Despite remarkable advances in understanding the mechanisms underlying inflammation, the currently available anti‐inflammatory therapies have many limitations, such as poor efficacies, low selectivity, and severe adverse effects. Bioactive materials with intrinsically anti‐inflammatory activities have emerged as promising drug candidates for the treatment of inflammatory disorders. Among them, nanotherapies based on bioactive cyclodextrin (CD) materials have attracted much attention, owing to their multiple advantages, including broad availability, well‐controlled structures, easy functionalization, good processibility, high cost‐effectiveness, and excellent biocompatibility. This review provides a comprehensive overview of the recent advancements in the development and applications of anti‐inflammatory nanoparticles (NPs) based on bioactive CD materials, with special focus on reactive oxygen species‐scavenging NPs and NPs capable of regulating inflammatory cell recruitment and activation. In addition, the applications of these anti‐inflammatory nanotherapies in the treatment of different acute/chronic inflammatory diseases are highlighted. Furthermore, major challenges in the clinical translation of these new generation anti‐inflammatory therapies derived from bioactive CD materials are discussed.

show abstract

Cyclodextrin polysulphates repress IL-1 and promote the accumulation of chondrocyte extracellular matrix

Abstract: CDPS is a novel polysulfated polysaccharide showing cartilage structure modifying effects in vitro as it improves the synthesis of aggrecan and the accumulation of CAM macromolecules. This effect probably resulted in part from the downregulation of IL-1.

Cited by 8 publications

References 39 publications

<title>Effect of LED irradiation on the expression of MMP-3 and MMP-13 in SW1353 cells in vitro</title>

<title>Effect of LED irradiation on the expression of MMP-3 and MMP-13 in SW1353 cells in vitro</title>

Optimized alkylated cyclodextrin polysulphates with reduced risks on thromboembolic accidents improve osteoarthritic chondrocyte metabolism

Anti‐Inflammatory Nanotherapies Based on Bioactive Cyclodextrin Materials

Contact Info

Product

Resources

About