Cyclodextrin production by Bacillus firmus strain 37 cells immobilized on loofa sponge

Pazzetto, Rúbia; Delani, Tieles Carina de Oliveira; Fenelon, Vanderson Carvalho; Matioli, Graciette

doi:10.1016/j.procbio.2010.07.008

Cited by 30 publications

(37 citation statements)

References 21 publications

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“…While existing literature does not include reports on the use of immobilization in loofa sponge for the production of enzymes from microorganisms of the Alicyclobacillus genus, there are studies Lineweaver-Burk (b) on the production of enzymes and polysaccharides using a variety of immobilized microorganisms. Pazzetto et al, 22 evaluated the production of β-CD by strain 37 immobilized Bacillus firmus in similar matrices to the present study, and immobilization in loofa sponge and alginate-sponge provided better results than with free cells.…”

Section: Production Of Collagenase By Alicyclobacillus Sendaiensis Kcsupporting

confidence: 58%

“…The loofa sponge, obtained from the dry seedless fruit of Luffa cylindrica, was used as an immobilization support and was prepared in accordance with the methods described by Pazzetto et al 22 For the cell immobilization process by adsorption, three loofa sponge discs were added to the culture medium containing the reactivated microbial cells. The culture was maintained by incubation for 72 h and 150 rpm.…”

Section: Production Of Enzymes By Alicyclobacillus Acidocaldarius Andmentioning

confidence: 99%

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Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

et al. 2017

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The biosynthesis of amylase and collagenase, produced by A. acidocaldarius and A. sendaiensis respectively, were studied, and different matrices evaluated for the microorganisms immobilization and enzymes production optimization, such as loofa sponge, alginate-sponge and alginate, followed by concentration through ultrafiltration. Using a wheat bran substrate, the amylase enzyme displayed enzymatic activity of 0.45 U mL -1 and optimum temperature and pH conditions of 75 °C and pH 3.0, respectively. Thermal stability was in the range of 55 to 60 °C. The apparent Km and Vmax were 3.2 mg mL -1 and 0.5 U mL -1, respectively. The production of collagenase by A. sendaiensis was carried out with potato dextrose broth substrate and the activity obtained was 7.2 U mL -1 . For amylase, the best results were obtained from immobilization in loofa sponge and the use of ultrafiltration (0.67 U mL -1) and for the collagenase extract, from the free biomass and ultrafiltration (13.6 U mL -1 ). The use of an ultrafiltration system enabled an average increase of 54% in the activity of both enzymes. Therefore, Alicyclobacillus are capable of producing enzymes of industrial interest, with the possibility of economically viable application of the substrate, and the use of immobilization and ultrafiltration produced positive results.

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Section: Production Of Collagenase By Alicyclobacillus Sendaiensis Kcsupporting

confidence: 58%

Section: Production Of Enzymes By Alicyclobacillus Acidocaldarius Andmentioning

confidence: 99%

Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

et al. 2017

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“…The use of matrices that do not impose such problems are of great benefit and will probably result in better productivities. Pazzetto [21] showed that diffusional problems are severe when alginate beads were used for the immobilization of Bacillus firmus, differently from the experiments carried with sponge plant, which is a highly porous material. Based on these observations, different aeration rates were evaluated for the production of CGTase with Bacillus firmus immobilized in bovine bone charcoal.…”

Section: Introductionmentioning

confidence: 96%

Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Silva¹,

Bieli²,

Valarini³

et al. 2018

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The process described in the present work uses air supplementation in a fluidized bed reactor containing Bacillus firmus strain 37 immobilized on active bovine bone charcoal, to produce by batch fermentation the enzyme CGTase (cyclomaltodextrin-glucanotransferase). Three different aeration rates were evaluated. The maximum CGTase activity was achieved after 120 hours of fermentation with aeration rate of 2 vvm and was equal to 2.48 U/mL. When 0.5 and 1 vvm were used the enzymatic activities achieved 1.1 and 0.57 U/mL, respectively. Bovine bone charcoal was characterized in terms of surface area, pore size and volume. To the best of our knowledge, the immobilization of microorganism cells in bovine bone charcoal for CGTase production has not been reported in the literature. Our results showed that fluidized bed reactor allows retaining high concentration of biomass, improving biomass-substrate contact and operation at low residence times, which resulted in improved enzyme production. Therefore, the process as proposed has great potential for industrial development.

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“…They are produced during degradation of starch by cyclodextrin glucanotransferases (CGTase) (EC 2.4.1.19) which is commercially available or can be produced by some microorganisms especially diverse strains of Bacillus such as Bacillus circulans (1). Bacillus macerans, Bacillus firmus (2) and Bacillus licheniformis (3)(4)(5). The capability of cyclodextrins (CDs) to form inclusion complexes and modify the physical and chemical properties of the guest molecules, makes them extremely attractive for vast industrial applications such as food, chemical, pharmaceutical, and textile, as well as in biotechnology and agriculture.…”

Section: Introductionmentioning

confidence: 99%

Modeling and Optimization of β-Cyclodextrin Production by Bacillus licheniformis using Artiﬁcial Neural Network and Genetic Algorithm

2013

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Background:The complexity of the fermentation processes is mainly due to the complex nature of the biological systems which follow the life in a non-linear manner. Joined performance of artificial neural network (ANN) and genetic algorithm (GA) in finding optimal solutions in experimentation has found to be superior compared to the statistical methods. Range of applications of β-cyclodextrin (β-CD) as an enzymatic derivative of starch is diverse, where the complex performance of cyclodextrin glucanotransferase (CGTase) as the involved enzyme is not well recognized. Objectives: The aim of the present work was to use ANN systems with different training algorithms and defined architectures joined with GA, in order to optimize β-CD production considering temperature of the reaction mixture, substrate concentration, and the inoculum's pH as the input variables. Materials and Methods: Commercially Neural Power, version 2.5 (CPC-X Software, 2004) was used for the numerical analysis according to the specifications provided in the software. β-CD concentration was determined spectrophotometrically according to phenolphthalein discoloration technique, described in the literature. Results: Randomly obtaining the experimental data for β-CD production in a fermentation process, could get explainable order using the ANN system coupled with GA. Changes of the β-CD as the function of each of the three selected input variables, were best quantified with use of the ANN system joined with the GA. The performance of the IBP learning algorithm was highly favorable (10300 epoch's number within 5 second, with the lowest RMSE value) while the sensitivity analysis of the results which was carried out according to the weight method, were indicative of the importance of input variables as follows: substrate concentration < temperature < inoculum's pH. For instance, small changes in the system's pH are associated with the large variation in the β-CD production as has been described by the suggested model. Conclusions: Production of β-CD (enzymatic derivative of starch) by B. licheniformis was satisfactorily described based on multivariate data analysis application of the ANN system and the experimental data were optimized by considering ANN plus the GA where the IBP was used as the training method and with use of three neurons as the constructed variables in the hidden layer of the test network.

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Cyclodextrin production by Bacillus firmus strain 37 cells immobilized on loofa sponge

Cited by 30 publications

References 21 publications

Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Modeling and Optimization of β-Cyclodextrin Production by Bacillus licheniformis using Artiﬁcial Neural Network and Genetic Algorithm

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Cyclodextrin production by Bacillus firmus strain 37 cells immobilized on loofa sponge

Cited by 30 publications

References 21 publications

Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

Biosynthesis of industrial enzymes by free and immobilized Alicyclobacillus in different matrices and the use of ultrafiltration in the enzymes concentration

Bovine Bone Charcoal as Support Material for Immobilization of &lt;em&gt;Bacillus firmus&lt;/em&gt; Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

Modeling and Optimization of β-Cyclodextrin Production by Bacillus licheniformis using Artiﬁcial Neural Network and Genetic Algorithm

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Bovine Bone Charcoal as Support Material for Immobilization of <em>Bacillus firmus</em> Strain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed