Cyclohexenonic long-chain fatty alcohol has therapeutic effects on diabetes-induced angiopathy in the rat aorta

Shinbori, Chiko; Saito, Motoaki; Kinoshita, Yukako; Satoh, Itaru; Kono, Tomoharu; Hanada, Takuya; Nanba, Eiji; Adachi, Kaori; Suzuki, Hiroto; Yamada, Masashi; Satoh, Keisuke

doi:10.1016/j.ejphar.2007.04.009

Cited by 16 publications

(19 citation statements)

References 32 publications

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“…Thus, the expression and activity of iNOS and COX-2 greatly modulate the synthesis of NO and prostaglandins and, hence, the inflammatory response. Shinbori et al [33] have recently reported that haxacosanol (C26) can improve diabetes-induced hypercontraction by down-regulation of iNOS expression. Our study demonstrates that LCFAs are able to inhibit nitrite production from LPS-stimulated RAW 264.7 macrophage culture without indication of cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Long-chain fatty alcohols from pomace olive oil modulate the release of proinflammatory mediators

Fernández-Arche

Márquez-Martín

Vázquez

et al. 2009

The Journal of Nutritional Biochemistry

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Pomace olive oil is a by-product of olive oil extraction that is traditionally produced and consumed in Spain. The nonglyceride matter of this oil is a good source of interesting minor compounds, like long-chain fatty alcohols, which are present free or as part of waxes. In the present study, long-chain fatty alcohols were isolated from the nonglyceride fraction of pomace olive oil, and the composition was identified and quantified. The major components of long-chain fatty alcohols were tetracosanol, hexacosanol and octacosanol. We investigated the ability of long-chain fatty alcohols from pomace olive oil to inhibit the release of different proinflammatory mediators in vitro by cells involved in inflammatory processes. Long-chain fatty alcohols significantly and dose-dependently decreased nitric oxide production by RAW 264.7 murine macrophages stimulated with lipopolysaccharide. Western blot analysis showed that nitric oxide reduction was a consequence of the inhibition of inducible nitric oxide synthetase expression. Long-chain fatty alcohols also reduced tumor necrosis factor-alpha and prostaglandin E(2) production, although the potency of inhibition for the latter was lower. On the other hand, long-chain fatty alcohols significantly reduced thromboxane A(2) production in rat peritoneal neutrophils stimulated with the calcium ionophore A-23187. The reduction of eicosanoid release was related to the inhibition of phospholipase A(2) enzyme activity by long-chain fatty alcohols, reaching an inhibitory concentration 50% value of 6.2 microg/ml. These results showed that long-chain fatty alcohols may have a protective effect on some mediators involved in the inflammatory damage development, suggesting its potential value as a putative functional component of pomace olive oil.

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Section: Discussionmentioning

confidence: 99%

Long-chain fatty alcohols from pomace olive oil modulate the release of proinflammatory mediators

Fernández-Arche

Márquez-Martín

Vázquez

et al. 2009

The Journal of Nutritional Biochemistry

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“…Real‐time PCR was performed according to our previous reports 22, 25. K IR 6.1 and K IR 6.2 mRNAs in the bladder were measured by real‐time PCR method.…”

Section: Methodsmentioning

confidence: 99%

Nicorandil ameliorates hypertension‐related bladder dysfunction in the rat

Saito

Ohmasa

Tsounapi

et al. 2012

Neurourology and Urodynamics

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“…Six-cording to our previous reports (23). The primer and probe of the β-actin used were from the LightCyclerPrimer/Probe Set (rat), and was used as the internal standard.…”

Section: Animal Modelsmentioning

confidence: 99%

“…Real-time PCR was performed according to our previous reports (23). Muscarinic M 3 receptor and eNOS mRNAs in the aorta were measured by realtime PCR method.…”

Section: Real-time Polymerase Chain Reaction (Pcr) Of Muscarinic M 3 mentioning

confidence: 99%

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Protective effect of taurine on diabetic rat endothelial dysfunction

et al. 2011

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As increasing evidence suggest that oxidative stress plays an important role in the developing angiopathy in diabetes, we studied the effects of taurine, a free radical scavenger, on diabetesinduced angiopathy in the rat aorta. Six-week-old male Wistar rats were randomly divided into three groups; control group (Cont), diabetes group (DM) and diabetes group treated with taurine for four weeks, 500 mg/kg/day, intraperitoneally (i.p.) (DM+T). Diabetes was induced by streptozotocin (50 mg/kg i.p.). Four weeks after the induction of diabetes, serum glucose and malondialdehyde concentrations were measured. Additionally, organ bath studies and real-time PCR on muscarinic M 3 receptor and eNOS were performed. Although taurine treatment failed to decrease serum glucose levels, the increased serum malondialdehyde levels in diabetic rats were significantly decreased after taurine treatment. Norepinephrine-induced hyper-contractility as well as acetylcholine-induced, endothelium-dependent hypo-relaxation in diabetes were significantly prevented after taurine treatment. The differences in the expressions of muscarinic M 3 receptor mRNAs were statistically non-significant between groups. Moreover, diabetes-induced up-regulation of eNOS mRNAs was slightly prevented after taurine treatment. These data suggest that taurine acts beneficially against the diabetes-induced vascular dysfunction. Its potential action as a radical scavenger ameliorates the vascular disorders in diabetes.Vascular disease is a prominent sequela of both type-1 and type-2 diabetes (7). Cardiovascular disease alters vascular responsiveness to several vasoconstrictors and vasodilators and is a major factor underlying the development of this disease (21). Most of the complications in diabetes are caused by the increased serum glucose and the increased generation of oxygen-derived free radicals, which lead to endothelium dysfunction and neuropathy. Although strict glycemic control delays the onset and slows down the progression of diabetic vascular complications (8, 25), this strategy is not successful in all patients. It has been demonstrated that there are functional changes in various smooth muscle cells in diabetic animals (16). Oxidative stress plays a pivotal role in the development of diabetes complications, both microvascular and cardiovascular. The metabolic abnormalities of diabetes cause mitochondrial superoxide overproduction in endothelial cells of both large and small vessels, as well as in the myocardium. The increased superoxide production causes the activation of five major pathways involved in the pathogenesis of microvascular and cardiovascular damage: polyol pathway flux, increased formation of advanced glycation end products (AGEs), increased expression of the receptor for AGEs and its activating ligands, activation of protein kinase C

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Cyclohexenonic long-chain fatty alcohol has therapeutic effects on diabetes-induced angiopathy in the rat aorta

Cited by 16 publications

References 32 publications

Long-chain fatty alcohols from pomace olive oil modulate the release of proinflammatory mediators

Long-chain fatty alcohols from pomace olive oil modulate the release of proinflammatory mediators

Nicorandil ameliorates hypertension‐related bladder dysfunction in the rat

Protective effect of taurine on diabetic rat endothelial dysfunction

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