Cycloheximide and Cortisol Inhibition of Estradiol-Stimulated Uterine Uptake and Distribution of Homologous Serum Albumin and Alpha Globulin in the Rat<sup>1</sup>

Peterson, Roy P.; Spaziani, Eugene

doi:10.1210/endo-85-5-932

Cited by 18 publications

(4 citation statements)

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“…The findings suggest that uterine blood volume can be used as a quantitative index of the changes in uterine circulation associated with estrogen-induced hyperemia. Other studies have indicated that uterine blood flow increases in rodents following estrogen administration (9,13,16). It is clear that uterine blood volume changes, although corresponding in time to measured changes in uterine blood flow, do not represent in a direct way changes in flow.…”

Section: Discussionmentioning

confidence: 95%

Determination of Uterine Blood Volume and Correlation with Ovarian Function

Brody

Clark

Edvinsson

et al. 1974

Experimental Biology and Medicine

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Section: Discussionmentioning

confidence: 95%

Determination of Uterine Blood Volume and Correlation with Ovarian Function

Brody

Clark

Edvinsson

et al. 1974

Experimental Biology and Medicine

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“…Our method of labelling the tissues removed a large part of the non-specific material, which explains the absence of non-specific, binding of [6,7_3H]oestradiol-17fl in immature uterus at high concentrations of [6,7-3H]oestradiol-17,. [6,7-3H]Oestradiol-17# equilibrated with serum was not precipitated by protamine so the 4S component is unlikely to be due to non-specific binding, although one cannot rule out the possibility of selective uptake of the serum oestradiol-17,-binding protein by the mature uterus (Peterson & Spaziani, 1969). This is, however, unlikely as the evidence suggests that the oestradiol-17,-binding protein in serum is similar to the testosterone-binding globulin (Rosner & Deakins, 1968) and our experiments indicated no binding of testosterone.…”

Section: Resultsmentioning

confidence: 99%

The use of protamine to study [6,7-3H]-oestradiol-17β binding in rat uterus

Steggles

King

1970

Biochemical Journal

135

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1. The binding of [6,7-(3)H]oestradiol-17beta to uteri has been studied by using sucrose-gradient analysis and also the property of oestradiol receptors to form insoluble complexes with protamine. 2. Protamine precipitates the 8S and part of the 4S oestradiol-binding proteins in uterine cytoplasm from mature rats. It does not precipitate the oestradiol-17beta-binding proteins present in cytoplasm from non-target tissues or serum. No tritium-labelled material was precipitated by protamine after equilibration of [6,7-(3)H]oestradiol-17beta with either serum albumin or phosvitin. 3. Protamine precipitated a small amount of progesterone but not testosterone or cortisol that had been equilibrated with uterine cytoplasm. It did not precipitate any tritium radioactivity from muscle cytoplasm that had been equilibrated with either [1,2-(3)H]testosterone sulphate or [1,2-(3)H]dehydroepiandrosterone. 4. A simple method has been devised for measuring binding constants of tissue extracts for [6,7-(3)H]oestradiol-17beta, based on precipitation with protamine. Reasonable agreement was obtained between the values obtained by this method and those obtained by sucrose-gradient analysis. 5. This method has been used to study the effect of maturity, ovariectomy, adrenalectomy and hypophysectomy on the cytoplasmic binding of [6,7-(3)H]oestradiol-17beta. None of these procedures affected the dissociation constant K(d) or the number of binding sites/mg of cytoplasmic protein. When measured per uterus or per mg of DNA, ovariectomy and hypophysectomy decreased the number of binding sites. Adrenalectomy had no effect. 6. The properties of the 4S oestradiol-binding protein present in cytoplasm from mature uteri have been studied. It is not present in uteri from immature, ovariectomized, or hypophysectomized rats and it does not bind testosterone or cortisol. Unlabelled oestradiol-17beta, U-11,100A, N-ethylmaleimide and N-bromosuccinimide all decrease the binding of [6,7-(3)H]oestradiol-17beta to both 8S and 4S receptors. Binding to both 8S and 4S receptors decreases when oestradiol is transported to the nucleus. The 4S receptor is not the same as the 4S binding component formed by salt dissociation of the 8S receptor.

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“…An increased blood volume is observed within 30-45 min in rats (e.g. Peterson & Spaziani 1969) and ewes (e.g. Huckabee et al 1970;Killam et al 1973).…”

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confidence: 99%

“…The acute effect of oestrogen on the uterus of the spayed animal can be divided into two phases; firstly an early response of increased blood flow, microvascular permeability and oedema (Peterson & Spaziani 1969) and, secondly an anabolic phase beginning after about 10 h with an increase in dry weight. The early, vascular response is apparent shortly after injection.…”

mentioning

confidence: 99%

Oestrogens and anti-oestrogens show dissociation between early uterine vascular responses and uterotrophic effects in mice

Arvidson

Terenius

1982

Acta Endocrinologica

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The structure-activity relationships of various natural and synthetic oestrogens, anti-oestrogens and some other steroid hormones with respect to early uterine albumin accumulation (EAV) was studied in spayed mice. The results were compared with the activity of these agents to produce a late anabolic response (LAR). The potency ranking order among the oestrogens with respect to EAV was found to be oestradiol-17\g=b\, > oestriol, > meso-hexoestrol, > erythro-deoxyhexoestrol, > ent-oestradiol-17\g=b\, > (-)methallenestril phenol. Generally the oestrogens were much more potent with respect to EAV than LAR. No correlation was found when the ability to promote EAV and LAR was compared. Among other agents tested for effect on the EAV, progesterone, the synthetic progestogen, DU-41165 and the anti-hormones MER-25 and U-11100A (nafoxidine) showed no effect. However, hydrocortisone diminished EAV, while dihydrotestosterone increased EAV substantially.

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Cycloheximide and Cortisol Inhibition of Estradiol-Stimulated Uterine Uptake and Distribution of Homologous Serum Albumin and Alpha Globulin in the Rat¹

Cited by 18 publications

References 0 publications

Determination of Uterine Blood Volume and Correlation with Ovarian Function

Determination of Uterine Blood Volume and Correlation with Ovarian Function

The use of protamine to study [6,7-3H]-oestradiol-17β binding in rat uterus

Oestrogens and anti-oestrogens show dissociation between early uterine vascular responses and uterotrophic effects in mice

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Cycloheximide and Cortisol Inhibition of Estradiol-Stimulated Uterine Uptake and Distribution of Homologous Serum Albumin and Alpha Globulin in the Rat1

Cited by 18 publications

References 0 publications

Determination of Uterine Blood Volume and Correlation with Ovarian Function

Determination of Uterine Blood Volume and Correlation with Ovarian Function

The use of protamine to study [6,7-3H]-oestradiol-17β binding in rat uterus

Oestrogens and anti-oestrogens show dissociation between early uterine vascular responses and uterotrophic effects in mice

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Cycloheximide and Cortisol Inhibition of Estradiol-Stimulated Uterine Uptake and Distribution of Homologous Serum Albumin and Alpha Globulin in the Rat¹