Cyclooxygenase-1 as a Potential Therapeutic Target for Seizure Suppression: Evidences from Zebrafish Pentylenetetrazole-Seizure Model

Barbalho, Patrícia Gonçalves; Carvalho, Benilton; Lopes-Cendes, Íscia; Maurer-Morelli, Cláudia Vianna

doi:10.3389/fneur.2016.00200

Cited by 22 publications

(11 citation statements)

References 59 publications

(74 reference statements)

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“…Prior to pretreatment, a toxicology evaluation may be performed to determine the maximum-tolerated concentrations (MTCs) of the drugs utilized, to avoid toxicity. This can be performed by using test compounds and observing the effects of AEDs on affected zebrafish for a period of 24 h (Barbalho et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

A Systematic Review on Non-mammalian Models in Epilepsy Research

et al. 2018

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Epilepsy is a common neurological disorder characterized by seizures which result in distinctive neurobiological and behavioral impairments. Not much is known about the causes of epilepsy, making it difficult to devise an effective cure for epilepsy. Moreover, clinical studies involving epileptogenesis and ictogenesis cannot be conducted in humans due to ethical reasons. As a result, animal models play a crucial role in the replication of epileptic seizures. In recent years, non-mammalian models have been given a primary focus in epilepsy research due to their advantages. This systematic review aims to summarize the importance of non-mammalian models in epilepsy research, such as in the screening of anti-convulsive compounds. The reason for this review is to integrate currently available information on the use and importance of non-mammalian models in epilepsy testing to aid in the planning of future studies as well as to provide an overview of the current state of this field. A PRISMA model was utilized and PubMed, Springer, ScienceDirect and SCOPUS were searched for articles published between January 2007 and November 2017. Fifty-one articles were finalized based on the inclusion/exclusion criteria and were discussed in this review. The results of this review demonstrated the current use of non-mammalian models in epilepsy research and reaffirmed their potential to supplement the typical rodent models of epilepsy in future research into both epileptogenesis and the treatment of epilepsy. This review also revealed a preference for zebrafish and fruit flies in lieu of other non-mammalian models, which is a shortcoming that should be corrected in future studies due to the great potential of these underutilized animal models.

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Section: Resultsmentioning

confidence: 99%

A Systematic Review on Non-mammalian Models in Epilepsy Research

et al. 2018

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“…Current evidence is inconclusive on the effectivity of COX-2 therapy, and many have reported that the effects of COX-2 therapy may be highly dependent on the type of inhibitor used and the timing at which the medication is administered in relation to seizure onset. Such conflicting results are drawing attention to other targets within PG synthesis and mechanistic pathways [ 43 ]. COX-2 is upregulated by platelet-activating factor (PAF) and NMDA activation [ 7 , 44 ].…”

Section: Inflammation In the Central Nervous Systemmentioning

confidence: 99%

The role of inflammation in the development of epilepsy

Rana

Musto

2018

J Neuroinflammation

513

328

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Epilepsy, a neurological disease characterized by recurrent seizures, is often associated with a history of previous lesions in the nervous system. Impaired regulation of the activation and resolution of inflammatory cells and molecules in the injured neuronal tissue is a critical factor to the development of epilepsy. However, it is still unclear as to how that unbalanced regulation of inflammation contributes to epilepsy. Therefore, one of the goals in epilepsy research is to identify and elucidate the interconnected inflammatory pathways in systemic and neurological disorders that may further develop epilepsy progression. In this paper, inflammatory molecules, in neurological and systemic disorders (rheumatoid arthritis, Crohn’s, Type I Diabetes, etc.) that could contribute to epilepsy development, are reviewed.Understanding the neurobiology of inflammation in epileptogenesis will contribute to the development of new biomarkers for better screening of patients at risk for epilepsy and new therapeutic targets for both prophylaxis and treatment of epilepsy.

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“…The same problem happened in the zebrafish model for epilepsy. Since the establishment of the PTZ-induced seizure model for epilepsy in zebrafish (Baraban et al, 2005), there have been rich applications (Afrikanova et al, 2013; Banote et al, 2013; Barbalho et al, 2016; Berghmans et al, 2007; Gupta et al, 2014; Hunyadi et al, 2017; Lin et al, 2018; Liu and Baraban, 2019; Mei et al, 2013; Mussulini et al, 2013; Pineda et al, 2011; Siebel et al, 2013; Tiedeken and Ramsdell, 2007; Turrini et al, 2017; Yang et al, 2017). However, the seizures induced by PTZ only cause transient acute seizures, which have poor face validity as the defining feature of epilepsy is spontaneous recurrent seizures (Johan Arief et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Once Stage III is evoked in zebrafish, it cannot be reversed by any method except the administration of AEDs (Baraban et al, 2005). Since this first demonstration of the zebrafish larval model, seizure-like responses have been described by behavioral changes in locomotor activity (Baraban et al, 2005; Barbalho et al, 2016; Berghmans et al, 2007), EEG (Pineda et al, 2011), c-fos expression (Baraban et al, 2005; Barbalho et al, 2016), optical imaging (Turrini et al, 2017), calcium imaging (Liu and Baraban, 2019), and the phosphorylation of ERK1/2 kinases (Randlett et al, 2015). The same PTZ treatment had been proven to be applicable to the adult zebrafish, and the behavioral response profile was characterized by stages that were comparable to those described for the rodent models (Afrikanova et al, 2013; Gupta et al, 2014; Mussulini et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A pentylenetetrazole-induced kindling zebrafish larval model for evoked recurrent seizures

Sun

Zhu²,

et al. 2019

Preprint

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Background:Transient pentylenetetrazol (PTZ) treatment on zebrafish larvae has been widely accepted a promising animal model for human epilepsy. However, this model is not ideal due to its acuteness and lack of recurrent seizures, which are the key feature of epilepsy in human disease. It is important to develop a more sensitive zebrafish model for epilepsy with well-controlled, predictable, recurrent seizures. New Method:The new method includes an experimental setup and a treatment protocol. The setup tracks the locomotion activity of up to 48 larvae simultaneously, while a visual stimulus can be presented to each of the 48 animals individually. The protocol treated the larvae through a water bath in 5 mM PTZ while being stimulated with rotating grating stimuli for 1 hour/day from 5 to 7 days postfertilization. Results:The setup captured the locomotion activity of zebrafish larvae during visual stimulation. The new protocol generated recurrent responses after flashing lights 4 hours post PTZ treatment. The effects could be suppressed by the anti-epileptic drug valproic acid. The characteristics of the visual stimulus play a major role in this kindling model. Comparisons with Existing Methods:We compared the proposed method with the transient PTZ model and confirmed that the flashing-light-evoked recurrent seizure is a new feature in addition to the transient changes. Conclusions:The new method generated non-drug-triggered predictable recurrent seizures in response to intermittent photic stimulation in zebrafish larvae and may serve as a sensitive method for anti-epileptic drug screening or a new research protocol in epilepsy research. Baraban, 2015; Liu and Baraban, 2019). In the acute seizure zebrafish model, lower concentrations of pentylenetetrazol (PTZ) merely evoked seizure-like behavior, such as increased locomotion activity (Stage I and II) and clonus-like convulsion (Stage III), which can be evoked only in the presence of high concentrations of PTZ (15 mM). Once Stage III is evoked in 2005; Grone and

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Cyclooxygenase-1 as a Potential Therapeutic Target for Seizure Suppression: Evidences from Zebrafish Pentylenetetrazole-Seizure Model

Cited by 22 publications

References 59 publications

A Systematic Review on Non-mammalian Models in Epilepsy Research

A Systematic Review on Non-mammalian Models in Epilepsy Research

The role of inflammation in the development of epilepsy

A pentylenetetrazole-induced kindling zebrafish larval model for evoked recurrent seizures

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