Cyclooxygenase-2 Unlike Cyclooxygenase-1 is Highly Expressed in Ovine Embryos during the Implantation Period1

Charpigny, Gilles; Reinaud, Pierrette; Tamby, Jean-Philippe; Créminon, Christophe; Guillomot, Michel

doi:10.1095/biolreprod57.5.1032

Cited by 110 publications

(89 citation statements)

References 64 publications

(96 reference statements)

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“…The termination of PTGS2 expression on the embryo coincides with implantation in sheep (Charpigny et al 1997). Consistent with our data of no further rise after days 15-18, day 19 bovine blastocysts secreted PG less abundantly than those of day 16 (Lewis et al 1982), obviously peaking around day 18 (Wilson et al 1992) depending on the specific PG in focus.…”

Section: Prostaglandins In the Bovine Uterussupporting

confidence: 88%

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Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Ulbrich¹,

Schülke²,

Groebner³

et al. 2009

Reproduction

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Prostaglandins (PGs) are important regulators of reproductive processes including early embryonic development. We analyzed the most relevant PG in bovine uteri at different preimplantation pregnancy stages when compared with non-pregnant controls. Additionally, endometrium and trophoblast tissues were examined regarding specific enzymes and receptors involved in PG generation and function. Simmental heifers were artificially inseminated or received seminal plasma only. At days 12, 15, or 18, post-estrus uteri were flushed for PG determination by liquid chromatography-tandem mass spectrometry. Endometrium and trophoblast tissues were sampled for RNA extraction and quantitative real-time PCR analysis. At all days and points of time examined, the concentration of 6-keto PGF 1a (stable metabolite of PGI 2 ) was predominant followed by PGF 2a OPGE 2 OPGD 2 zTXB 2 (stable metabolite of TXA 2 ). At days 15 and 18, PG increased from overall low levels at day 12, with a much more pronounced increase during pregnancy. The PGF 2a /PGE 2 ratio was not influenced by status. The highest PG concentration was measured at day 15 with 6-keto PGF 1a (6.4 ng/ml) followed by PGF 2a (1.1 ng/ml) and PGE 2 (0.3 ng/ml). Minor changes in endometrial PG biosynthesis enzymes occurred due to pregnancy. Trophoblasts revealed high transcript abundance of general and specific PG synthases contributing to uterine PG. As PGI 2 and PGF 2a receptors were abundantly expressed by the trophoblast, abundant amounts of PGI 2 and PGF 2a in the uterine lumen point towards an essential role of PG for the developing embryo. High amounts of PG other than PGE 2 in the preimplantation uterus may be essential rather than detrimental for successful reproduction.

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Section: Prostaglandins In the Bovine Uterussupporting

confidence: 88%

“…PTGS2 was demonstrated in trophoblast cells (Charpigny et al 1997), and the supernatant of blastocysts contained considerable amount of PG in cattle and sheep (Marcus 1981, Charpigny et al 1997.…”

Section: Discussionmentioning

confidence: 98%

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Ulbrich¹,

Schülke²,

Groebner³

et al. 2009

Reproduction

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“…Prostaglandins have an important role in the regulation of the estrous cycle and successful implantation in pigs (Spencer and Bazer 2004). PTGS and PTGS2 are responsible for the formation of prostanoids, including prostaglandins (Sawdy et al 2000;Charpigny et al 1997). In our study, we noted the upregulation of PTGS2 caused by adiponectin treatment.…”

Section: Discussionsupporting

confidence: 57%

The influence of adiponectin on the transcriptomic profile of porcine luteal cells

Szeszko

Smolińska

Kieżun

et al. 2015

Funct Integr Genomics

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Reproductive functions are closely related to nutritional status. Recent studies suggest that adiponectin may be a hormonal link between them. Adiponectin is an adipocytokine, abundantly expressed in adipose tissues. It plays a dominant role in lipid and carbohydrate metabolism by stimulating fatty acid oxidation, decreasing plasma triglycerides, and increasing cells' sensitivity to insulin and has direct antiatherosclerotic effects. The hormone is also postulated to play a modulatory role in the regulation of the reproductive system. The aim of this study was to identify differentially expressed genes (DE-genes) in response to adiponectin treatment of porcine luteal ovarian cells. The global expression of genes in the porcine ovary was investigated using the Porcine (V2) Two-color gene expression microarray, 4 × 44 (Agilent, USA). Analysis of the microarray data showed that 701 genes were differentially expressed and 389 genes showed a fold change greater than 1.2 (p < 0.05). Among this number, 186 genes were up-regulated and 203 were down-regulated. The list of DE-genes was used for gene ontology analyses. The biological process list was generated from up-regulated and down-regulated DE-genes. We found that up-regulated products of DE-genes take part in 30 biological processes and down-regulated products in 9. Analysis of the interaction network among DE-genes showed that adiponectin interacts with genes involved in important processes in luteal cells. These results provide a basis for future work describing the detailed interactions and relationships explaining local regulation of adiponectin actions in the ovary of pigs.

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“…Expression of PTGS2 is more abundant in blastocysts resulting in calf delivery compared with those resulting in resorption (El-Sayed et al 2006). In bovine, the PTGS2 protein localizes to the TE, while the ICM does not express PTGS2 (Charpigny et al 1997), suggesting that this gene is necessary for the elongation and subsequent implantation. Moreover, higher expression of PTGS2 throughout the implantation window indicates an important role for the PGs released by the embryo in mediating interactions with the uterus (Charpigny et al 1997, Wang et al 2002.…”

Section: Development and Gene Expression In Parthenotesmentioning

confidence: 99%

“…In bovine, the PTGS2 protein localizes to the TE, while the ICM does not express PTGS2 (Charpigny et al 1997), suggesting that this gene is necessary for the elongation and subsequent implantation. Moreover, higher expression of PTGS2 throughout the implantation window indicates an important role for the PGs released by the embryo in mediating interactions with the uterus (Charpigny et al 1997, Wang et al 2002. As PTGS2 expression is time regulated during ruminant embryo development, differential expression could be related to asynchronous development between IVF and parthenogenetic embryos.…”

Section: Development and Gene Expression In Parthenotesmentioning

confidence: 99%

Biological differences between in vitro produced bovine embryos and parthenotes

Gómez¹,

Gutiérrez‐Adán²,

Díez³

et al. 2009

Reproduction

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Parthenotes may represent an alternate ethical source of stem cells, once biological differences between parthenotes and embryos can be understood. In this study, we analyzed development, trophectoderm (TE) differentiation, apoptosis/necrosis, and ploidy in parthenotes and in vitro produced bovine embryos. Subsequently, using real-time PCR, we analyzed the expression of genes expected to underlie the observed differences at the blastocyst stage. In vitro matured oocytes were either fertilized or activated with ionomycin C6-DMAP and cultured in simple medium. Parthenotes showed enhanced blastocyst development and diploidy and reduced TE cell counts. Apoptotic and necrotic indexes did not vary, but parthenotes evidenced a higher relative proportion of apoptotic cells between inner cell mass and TE. The pluripotence-related POU5F1 and the methylation DNMT3A genes were downregulated in parthenotes. Among pregnancy recognition genes, TP-1 was upregulated in parthenotes, while PGRMC1 and PLAC8 did not change. Expression of p66 shc and BAX/BCL2 ratio were higher, and p53 lower, in parthenotes. Among metabolism genes, SLC2A1 was downregulated, while AKR1B1, PTGS2, H6PD, and TXN were upregulated in parthenotes, and SLC2A5 did not differ. Among genes involved in compaction/blastulation, GJA1 was downregulated in parthenotes, but no differences were detected within ATP1A1 and CDH1. Within parthenotes, the expression levels of SLC2A1, TP-1, and H6PD, and possibly AKR1B1, resemble patterns described in female embryos. The pro-apoptotic profile is more pronounced in parthenotes than in embryos, which may differ in their way to channel apoptotic stimuli, through p66 shc and p53respectively, and in their mechanisms to control pluripotency and de novo methylation.Reproduction (2009) 137 285-295

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Cyclooxygenase-2 Unlike Cyclooxygenase-1 is Highly Expressed in Ovine Embryos during the Implantation Period1

Cited by 110 publications

References 64 publications

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

The influence of adiponectin on the transcriptomic profile of porcine luteal cells

Biological differences between in vitro produced bovine embryos and parthenotes

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