Cyclooxygenase as a Target in Chemoprevention by Probiotics During 1,2-Dimethylhydrazine Induced Colon Carcinogenesis in Rats

Walia, Sohini; Kamal, Rozy; Kanwar, Shamsher S.; Dhawan, D. K.

doi:10.1080/01635581.2015.1011788

Cited by 49 publications

(20 citation statements)

References 42 publications

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“…The dysbiotic environment produced by H. hepaticus is incompatible with L. plantarum through unknown mechanisms that may involve nutrient competition, susceptibility to toxins, or other environmental factors. Other studies have shown that adding L. plantarum reduces tumor size and burden in rats and inhibits survival of cancer stem cells ( 89 , 90 ). On the other hand, Lactobacillus murinus increases in all effects compared to control.…”

Section: Discussionmentioning

confidence: 98%

Functional Changes in the Gut Microbiome Contribute to Transforming Growth Factor β-Deficient Colon Cancer

et al. 2017

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Most research on the gut microbiome in colon cancer focuses on taxonomic changes at the genus level using 16S rRNA gene sequencing. Here, we develop a new methodology to integrate DNA and RNA data sets to examine functional shifts at the species level that are important to tumor development. We uncover several metabolic pathways in the microbiome that, when perturbed by host genetics and H. hepaticus inoculation, contribute to colon cancer. The work presented here lays a foundation for improved bioinformatics methodologies to closely examine the cross talk between specific organisms and the host, important for the development of diagnostics and pre/probiotic treatment.

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Section: Discussionmentioning

confidence: 98%

Functional Changes in the Gut Microbiome Contribute to Transforming Growth Factor β-Deficient Colon Cancer

et al. 2017

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“…The indigenous Lactobacillus plantarum (AdF10), isolated from traditional fermented food of Himachal Pradesh, exhibited highest protective attributes apart from its probiotic potential in our earlier studies (Sourabh, Kanwar, & Sharma, 2011;Walia, Kamal, Kanwar, & Dhawan, 2015;Walia, Keshani, Sood, & Kanwar, 2014). Hence, it is included as a test organism in this study to explore its best combination with prebiotics so that it could further be used in enriching the traditional fermented foods to harvest probiotic related health benefits or in the development of food products at the commercial scale.…”

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confidence: 99%

Effect of prebiotics on growth behavior of Lactobacillus plantarum and their impact on adherence of strict anaerobic pathogens to intestinal cell lines

Sharma

Kanwar

2017

Journal of Food Safety

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This study aimed to examine the influence of prebiotics on growth and activity of Lactobacillus plantarum and their effect on adherence of anaerobic pathogens to Caco-2 and HT-29 intestinal cell lines. The specific growth rate of indigenous L. plantarum in lactulose (0.51/hr) was found to be maximum followed by fructooligosaccharide (0.34/hr) and inulin (0.18/hr) which was statistically at par with reference strain. Shortest mean doubling time of indigenous strain was observed with lactulose (1 hr 35 min) followed by fructooligosaccharide (2 hr 3 min), and inulin (3 hr 85 min). Lactic and short-chain fatty acids namely, acetic, propionic, and butyric were the principle metabolites observed after fermentation of prebiotics as detected by ultra-performance liquid chromatography analysis. No significant change in growth and pH of anaerobic pathogens was observed in medium supplemented with different prebiotics. Presence of lactulose showed maximum inhibition in adherence of anaerobic pathogens namely, Peptostreptococcus anaerobius (39 and 37%), Clostridium perfringens (36 and 34%), and Bacteroides fragilis (18 and16%), to Caco-2 and HT-29 intestinal cell lines. Practical applicationsThe ability of certain bacteria to ferment prebiotics selectively is an important probiotic characteristic. According to the results of this study, lactulose was found to be the promising ingredient in combination with indigenous strain that not only supported the growth of lactobacillus strain, but also inhibited the adherence of anaerobic pathogens to intestinal cell lines. The metabolites produced on fermentation of prebiotic such as lactate, acetate, propionate, and butyrate have many reported health benefits in boosting the gut immunity of the host. Negligible growth of anaerobic pathogens in lactulose indicating its safe use without any ill effect to the host. Thus, the lactulose might have a potential for practical use in food industry for the development of future functional and dietary foods to impart health benefits at both colonic and systemic levels.

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“…34 Contrary to expectations, another implication was the possibility for the weight loss to originate from impaired host metabolism (glucose, lipids, and proteins). 35,36 As mentioned above, the use of probiotics could improve the weight loss caused by cancer. 37 This finding is consistent with those of many other studies suggesting the beneficial role of probiotics such as L. paracasei in enhancing metabolism, appetite, food intake, weight control, and been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Possible correlation between Lactobacillus paracasei X12 intake and tumor characteristics in the rat model of colorectal cancer

et al. 2020

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Introduction: There are many different therapeutic approaches to control colorectal cancer (CRC) which is recognized as one of the deadliest diseases in the world today. One of the most recent of which is the probiotic interventions to change the gut microbiome. Probiotics can be related to the control of gastrointestinal cancers in a variety of ways. Therefore, the present study aimed to investigate the relationship between supplement use with macroscopic and physiological changes of tumor in 1,2-dimethylhydrazine (DMH) -induced rats. Methods: The male Wistar rats were divided into three groups. Lactobacillus paracasei X12 was administrated (40 weeks) to the DMH-induced rats. DMH injection (30 mg/kg BW) was used for 12 weeks to induce CRC. The "AgNOR method" was applied for the evaluation of cell proliferation. Real-time polymerase chain reaction (PCR) was used to measure the gene expression of apoptotic markers. Results: The findings of this study indicated that L. paracasei X12 intake prevented weight loss caused by CRC (P=001), and probiotic consumption could significantly prevent tumor growth. Additionally, a significant correlation was observed between apoptosis markers and weight of animals, and a strong negative correlation (P=000) between apoptosis parameters and tumor characteristics (incidence, volume, and multiplicity of adenomas). A close association between cell proliferation and tumor characteristics was illuminated as well (P<001). Conclusion: This study revealed a strong correlation between tumor incidence and growth and probiotic intake in CRC. Moreover, it could be believed that cancer prevention is a far more essential and cost-effective way than its cure.

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Cyclooxygenase as a Target in Chemoprevention by Probiotics During 1,2-Dimethylhydrazine Induced Colon Carcinogenesis in Rats

Cited by 49 publications

References 42 publications

Functional Changes in the Gut Microbiome Contribute to Transforming Growth Factor β-Deficient Colon Cancer

Functional Changes in the Gut Microbiome Contribute to Transforming Growth Factor β-Deficient Colon Cancer

Effect of prebiotics on growth behavior of Lactobacillus plantarum and their impact on adherence of strict anaerobic pathogens to intestinal cell lines

Possible correlation between Lactobacillus paracasei X12 intake and tumor characteristics in the rat model of colorectal cancer

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