Cyclophosphamide-induced hepatotoxicity in wistar rats: The modulatory role of gallic acid as a hepatoprotective and chemopreventive phytochemical

Oyagbemi, Ademola Adetokunbo; Omobowale, Temidayo Olutayo; Asenuga, Ebunoluwa Racheal; Akinleye, Akinrinde Stephen; Ogunsanwo, Rachael Omolola; Saba, Adebowale Benard

doi:10.4103/2008-7802.177898

Cited by 41 publications

(25 citation statements)

References 43 publications

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“…Gallic was able to ameliorate the effect after four weeks of treatment and may be its effect would be better if the period of treatment was extended. This results came in agreement with (10,19) and l (32) .…”

Section: Discussionsupporting

confidence: 90%

The Effect of Gallic Acid as Protective Agent on Some Biochemical Parameters Induced Hypercholesterolemia in Laboratory Male Rats

AL-Taei¹,

AL-Diwan²

2017

Bas.J.Vet.Res.

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The role of gallic acid was studied to alleviating some biochemical parameters alterations in hyper cholesterolemia in rats. For this purpose 24 sexually adult male rats were divided randomly in to 4 groups (6 rats each). The experiment was conducted at the animal house of the Veterinary Medicine College-University of Basrah, the control group, rats were injected intraperitoneally (I.P.) with 0.9 % normal saline (N.S)and were supply with the stander ration. The first treated group were injected intraperitoneally (I.P.) with 100 mg/ kg gallic acid dissolve in 0.5 ml distilled water daily and were supply with the stander ration. The second treated group were supplied with the stander ration in addition to 1.5% cholesterol of the stander ration. The third treated group were supplied with standard ration in addition to 1.5% cholesterol, and injected intraperitoneally (I.P.) after one hour with 100 mg/kg gallic acid dissolved in 0.5 ml distilled water

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Section: Discussionsupporting

confidence: 90%

The Effect of Gallic Acid as Protective Agent on Some Biochemical Parameters Induced Hypercholesterolemia in Laboratory Male Rats

AL-Taei¹,

AL-Diwan²

2017

Bas.J.Vet.Res.

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“…Further studies confirm typical liver damage associated with CP administration [48][49][50]. Moreover, this was confirmed by other recent histopathological studies [47,[51][52][53], in which major CP induced histopathological alterations in liver tissue were periportal inflammation, inflammatory cells infiltration, hemorrhage, congestion of blood vessels, hepatocyte necrosis around the central veins, pyknosis of nuclei, cellular disintegration, and distortion.…”

Section: Discussionsupporting

confidence: 84%

Ameliorative Effect of Peach Seed Extract on Cyclophosphamide- Induced Cytogenetic and Histological Effects in Mice

Salah¹,

Aboul-ela²,

Swelim³

2019

IJGG

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Cyclophosphamide (CP) is a frequently used drug for its anticancer and immunosuppressive potential. However metabolism of CP in the body results into toxic chemical compounds (to the liver itself and other non-target vital organs) via oxidative stress, apoptosis induction and finally necrosis. Since there is no escaping of using such medications in spite of their harms this study was designed to access the ability to alleviate the side-effects of CP by using peach seed methanolic extract, due to its nutritional value and therapeutic properties. The peach seed extract has bioactive constituents such as phenols and carotenoids. Methanoloic extract is the most widely used since it offers a high recovery of antioxidant compounds. Mice were divided into five major groups: negative control (untreated group), positive control, was injected (IP) with CP in dose 75mg/kg b. wt., Third group received peach seed extract only in a dose of 500 mg/kg b.w., and the fourth and fifth groups received two doses of Peach seeds extract 500 and 250 mg/kg b.wt. after receiving a single dose of CP intraperitoneally. Assessment of the extract was performed using micronucleus test, mitotic chromosomal aberration assay using bone marrow cells, and liver samples were collected for histopathology. Our results demonstrated that CP induced highly significant e genotoxicity, which recorded 9.49% PE's with micronuclei, comparing to 1.03% as control, while the induction of chromosomal aberrations was recorded as 67.6% against 4.4% as negative control. The histological study on the liver cells recorded noticeable damage with liver cells treated with CP. After peach treatment a significant reduction in CP-induced damage was observed and those groups treated with both extract and CP became nearly similar to the untreated group in all tested parameters. peach methanolic seed extract has the potential to ameliorate the damaging effect of cyclophosphamide at both the genetic and histological levels.

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“…Lipid peroxidation in the bladder tissue was determined as MDA content by determining the thiobarbituric acid reactive substances 28 . Briefly, 0.2 ml of homogenate was mixed with 0.2 ml of 8.1% sodium dodecyl sulphate, 1.5 ml of 30% acetic acid (pH 3.5) and 1.5 ml of 0.8% thiobarbituric acid.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Thymoquinone against Cyclophosphamide-Induced Hemorrhagic Cystitis through Inhibiting DNA Damage and Upregulation of Nrf2 Expression

et al. 2016

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Cyclophosphamide (CYP) induced hemorrhagic cystitis is a dose-limiting side effect involving increased oxidative stress, inflammatory cytokines and suppressed activity of nuclear factor related erythroid 2-related factor (Nrf2). Thymoquinone (TQ), an active constituent of Nigella sativa seeds, is reported to increase the expression of Nrf2, exert antioxidant action, and anti-inflammatory effects in the experimental animals. The present study was designed to explore the effects of TQ on CYP-induced hemorrhagic cystitis in Balb/c mice. Cystitis was induced by a single intraperitoneal injection of CYP (200 mg/kg). TQ was administered intraperitoneally at 5, 10 and 20 mg/kg doses twice a day, for three days before and three days after the CYP administration. The efficacy of TQ was determined in terms of the protection against the CYP-induced histological perturbations in the bladder tissue, reduction in the oxidative stress, and inhibition of the DNA fragmentation. Immunohistochemistry was performed to examine the expression of Nrf2. TQ protected against CYP-induced oxidative stress was evident from significant reduction in the lipid peroxidation, restoration of the levels of reduced glutathione, catalase and superoxide dismutase activities. TQ treatment significantly reduced the DNA damage evident as reduced DNA fragmentation. A significant decrease in the cellular infiltration, edema, epithelial denudation and hemorrhage were observed in the histological observations. There was restoration and rise in the Nrf2 expression in the bladder tissues of mice treated with TQ. These results confirm that, TQ ameliorates the CYP-induced hemorrhagic cystitis in mice through reduction in the oxidative stress, inhibition of the DNA damage and through increased expression of Nrf2 in the bladder tissues.

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Cyclophosphamide-induced hepatotoxicity in wistar rats: The modulatory role of gallic acid as a hepatoprotective and chemopreventive phytochemical

Cited by 41 publications

References 43 publications

The Effect of Gallic Acid as Protective Agent on Some Biochemical Parameters Induced Hypercholesterolemia in Laboratory Male Rats

The Effect of Gallic Acid as Protective Agent on Some Biochemical Parameters Induced Hypercholesterolemia in Laboratory Male Rats

Ameliorative Effect of Peach Seed Extract on Cyclophosphamide- Induced Cytogenetic and Histological Effects in Mice

Protective Effect of Thymoquinone against Cyclophosphamide-Induced Hemorrhagic Cystitis through Inhibiting DNA Damage and Upregulation of Nrf2 Expression

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