Cyclosporine A-Encapsulated pH/ROS Dual-Responsive Nanoformulations for the Targeted Treatment of Colitis in Mice

Abstract

Inflammatory bowel disease (IBD) is a frequently occurring disease that seriously influences the patient’s quality of life. To decrease adverse effects and improve efficacy of therapeutics, nanomedicines have been widely used to treat IBD. However, how to thoroughly release payloads under an inflammatory microenvironment and synergistic therapy of IBD need to be further investigated. To address this issue, cyclosporine A (CsA)-loaded, folic acid (FA)-modified, pH and reactive oxygen species (ROS) dual-responsive nanoparticles (FA-CsA NPs) were fabricated using pH/ROS-responsive material as carrier. The prepared FA-CsA NPs had spherical shape and uniform size distribution and could smartly release their payloads under acid and/or ROS microenvironment. In vitro experiments demonstrated that FA-CsA NPs can be effectively internalized by activated macrophages, and the internalized NPs could down-regulate the expression of proinflammatory cytokines compared to free drug or nontargeted NPs. In vivo experiments verified that FA-CsA NPs significantly accumulated at inflammatory colon tissues and the accumulated NPs obviously improved the symptoms of colitis in mice without obvious adverse effects. In conclusion, our results provided a candidate for the targeted treatment of IBD.

Combined ROS Responsive Polydopamine-Coated Berberine Nanoparticles Effective Against Ulcerative Colitis in Mouse Model

“… 33 Achieving targeted and selective drug delivery through abnormally high concentrations of ROS at the site of inflammation can ensure that the drug is released only at the site of intestinal inflammation, not in healthy tissue. 34 , 35 Literature reports that disulfide bonds have ROS-responsive properties, and using D-α-tocopherol-polyethylene glycol succinate-poly (β-thioester) copolymer (TPGS-PBTE) to deliver luteolin can release luteolin at the lesion site with high ROS expression, helping to alleviate colonic inflammation and protect colonic tissue integrity. 8 Zhang et al developed an intelligent nano-therapy AON (nanoparticles based on β-cyclodextrin 4-phenylboronic acid pinocembrin ester, containing the membrane-degrading protein A1 mimetic peptide Ac2-26), achieving specific release and accumulation of Ac2-26 in the ROS environment.…”