In order to reduce the failure wear of rotating machinery, the subsequent economic loss and casualties, the remote vibration monitoring and fault diagnosis technology of rotating machinery based on B/S (browser/server) mode is studied. Firstly, the characteristics and structure of B/S mode, the remote vibration monitoring and fault diagnosis system of rotating machinery are analyzed and discussed. And then the system structure, flow chart, server and client design and implementation method are displayed. Subsequently, the overall scheme of rotating machinery fault diagnosis system based on B/S structure is designed. Furthermore, based on the idea of modularization, the scheme design of each module of the system is carried out, including network data acquisition and simulation experiment. Finally, the data and related information in the process of fault diagnosis are stored by analyzing the performance of database server, map refresh display and rotor phase. The remote monitoring system based on B/S can objectively reflect the vibration signal of rotating machinery, obtain the industrial field data in time, analyze the operation status of the equipment, find and correct the unreasonable elements in the design, and also show new design requirements and develop new products according to the actual needs of the site. It is helpful for on-site monitoring personnel to carry out routine monitoring and diagnosis of the equipment, saving about half of the human and material resources, and the results of remote monitoring are relatively accurate, which can diagnose the fault in time. Remote monitoring and fault diagnosis provide the first-hand information from the scene for scientific research institutes, and combine scientific research and production practice more closely. As a whole, large-scale rotating machinery is the key equipment in the production unit of enterprises. It is important to provide remote monitoring and fault diagnosis for enterprises. The achievements of remote monitoring and fault diagnosis system greatly improve the production efficiency.