Taking the combustor composite structure of a high-strength diesel engine as the main research object, dedicated tests have been conducted to verify the accuracy of three distinct cylinder gasket pressure simulation models. Using the measured cylinder gasket compression rebound curve, a gasket unit has been designed and manufactured. For this unit, the influence of the bolt pretension, cylinder body and cylinder head material on gasket sealing pressure has been investigated systematically in conditions of thermo-mechanical coupling. The results show that the bolt pretension force is one of the most important factors affecting the cylinder gasket sealing pressure. The change of the body material has little effect on this pressure. The cylinder gasket seal pressure decreases progressively with the reduction of the elastic module of the cylinder head material.