Cymbaria daurica L.: A Mongolian herbal medicine for treating eczema via natural killer cell-mediated cytotoxicity pathway

Huang, Congying; Li, Siqi; Guo, Weisi; Zhang, Ziyan; Meng, Xiangxi; Li, Xing; Gao, Bing; Wen, Rong; Niu, Hui; Zhang, Chunhong; Li, Min-Hui

doi:10.1016/j.jep.2023.116246

Cited by 5 publications

(4 citation statements)

References 53 publications

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“…The spleen index is an important index for reflecting the immune function of the body [42]. Eczema will lead to abnormal immune function in mice, which will lead to an increase in the spleen index [43]. Berberine hydrochloride could reduce the spleen index increased by eczema, and it had the same curative effect as DEX.…”

Section: Ear Swellingmentioning

confidence: 99%

Evaluation of Antioxidant Activity and Treatment of Eczema by Berberine Hydrochloride-Loaded Liposomes-in-Gel

Shen,

Qu,

Liu

et al. 2024

Molecules

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In this paper, berberine hydrochloride-loaded liposomes-in-gel were designed and developed to investigate their antioxidant properties and therapeutic effects on the eczema model of the mouse. Berberine hydrochloride-liposomes (BBH-L) as the nanoparticles were prepared by the thin-film hydration method and then dispersed BBH-L evenly in the gel matrix to prepare the berberine hydrochloride liposomes-gel (BBH-L-Gel) by the natural swelling method. Their antioxidant capacity was investigated by the free radical scavenging ability on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and H2O2 and the inhibition of lipid peroxides malondialdehyde (MDA). An eczema model was established, and the efficacy of the eczema treatment was preliminarily evaluated using ear swelling, the spleen index, and pathological sections as indicators. The results indicate that the entrapment efficiency of BBH-L prepared by the thin-film hydration method was 78.56% ± 0.7%, with a particle size of 155.4 ± 9.3 nm. For BBH-L-Gel, the viscosity and pH were 18.16 ± 6.34 m Pas and 7.32 ± 0.08, respectively. The cumulative release in the unit area of the in vitro transdermal study was 85.01 ± 4.53 μg/cm2. BBH-L-Gel had a good scavenging capacity on DPPH and H2O2, and it could effectively inhibit the production of hepatic lipid peroxides MDA in the concentration range of 0.4–2.0 mg/mL. The topical application of BBH-L-Gel could effectively alleviate eczema symptoms and reduce oxidative stress injury in mice. This study demonstrates that BBH-L-Gel has good skin permeability, excellent sustained release, and antioxidant capabilities. They can effectively alleviate the itching, inflammation, and allergic symptoms caused by eczema, providing a new strategy for clinical applications in eczema treatment.

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Section: Ear Swellingmentioning

confidence: 99%

Evaluation of Antioxidant Activity and Treatment of Eczema by Berberine Hydrochloride-Loaded Liposomes-in-Gel

Shen,

Qu,

Liu

et al. 2024

Molecules

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“…Хоча відомості про контактну гіперчутливість отримані в основному з мишачої моделі, щури як модель для дослідження контактної алергії також використовуються щодо вивчення залучених до патогенезу АД запальних та імунних механізмів [46]. Модель атопічного дерматиту, індукованого в щурів 2,4-динітрохлорбензолом не йде всупереч етіопатогенетичним принципам розвитку цього захворювання у людей і, як наслідок, експлуатується в багатьох експериментальних дослідженнях [47][48][49].…”

Section: обговоренняunclassified

Cord Blood as a Corrector of Hematological and Cytomorphological Characteristics of Lymphohemopoietic Organs in Experimental Atopic Dermatitis

Koval,

Bondarovych,

Goltsev

2023

Innov Biosyst Bioeng

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Background. Atopic dermatitis (AD) is an inflammatory skin disease caused by a shift in the interaction between cells of the immune system and the skin. Steroid anti-inflammatory drugs used to treat AD often lead to numerous side effects. Therefore, the development of new natural multifunctional preparations with immunomodulatory activity is of great importance. Objective. To determine the changes in indices of the leukocytes cluster of peripheral blood and determine the peculiarities of cytomorphological changes in the spleen and lymph nodes (LN) of rats with induced AD before and after administration of cryopreserved (cHCBL) and lyophilized (lHCBL) forms of human cord blood leukoconcentrate. Methods. The experiments were conducted using 6-month-old Wistar rats. AD was induced by topically applying a 5% alcohol-acetone solution of dinitrochlorobenzene (DNCB) to the skin of the rats' back for 21 days. Each form of the preparation (cHCBL and lHCBL) was administered intraperitoneally in a 0.5 ml dose containing 5´106 cells, one day after the final DNCB treatment. The following parameters were assessed in the blood: leukocyte count and the neutrophil-to-lymphocyte ratio (N/L index). Сytomorphological characteristics were determined in the spleen and inguinal LN. Results. In AD, specific changes in the cytomorphological characteristics of the lymphohematopoietic complex (LHС) structures, namely the spleen and LN, were observed alongside alterations in the leukocyte cluster indices. The most notable changes in the spleen included the dominance of the white pulp over the red pulp and the prevalence of follicles of stages II and III of development in the LN. Animals with AD displayed pronounced leukocytosis and an increased N/L index in their blood. The study demonstrates the positive corrective effects of cHCBL and lHCBL on the morphological characteristics of LHC and the specified blood parameters. Conclusions. The effectiveness of the cHCBL and lHCBL use for correcting indices of leukocytes cluster of peripheral blood and the structural organization of lymphohemopoietic organs in the development of AD have been proven.

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“…The dried whole plant is also included in several classical herbal formulations, such as “ Siweixinbasan ” and “ Baweixinbasan .” Previous studies have shown that C. daurica contains 177 chemical components (Wu et al., 2020 ), being the most common flavonoids (Li et al., 2014 ) and iridoid glycosides (Wang et al., 2018 ). In modern pharmacological research, the herb has received much recent attention owing to its antidiabetic (Gong et al., 2020 ; Shi, Chen, et al., 2023 ; Shi, Li, et al., 2023 ) and anti‐inflammatory (Guo et al., 2017 ; Huang et al., 2023 ) properties. However, either accidental or intentional, the adulteration and substitution of C. daurica frequently occurs in China and Mongolia.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have shown that C. daurica contains 177 chemical components (Wu et al, 2020), being the most common flavonoids (Li et al, 2014) and iridoid glycosides (Wang et al, 2018). In modern pharmacological research, the herb has received much recent attention owing to its antidiabetic (Gong et al, 2020;Shi, Chen, et al, 2023; and antiinflammatory (Guo et al, 2017;Huang et al, 2023) properties.…”

Section: Introductionmentioning

confidence: 99%

Complete chloroplast genomes of the hemiparasitic genus Cymbaria: Insights into comparative analysis, development of molecular markers, and phylogenetic relationships

Ma,

López‐Pujol,

Yan

et al. 2024

Ecology and Evolution

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The hemiparasitic tribe Cymbarieae (Orobanchaceae) plays a crucial role in elucidating the initial stage of the transition from autotrophism to heterotrophism. However, the complete chloroplast genome of the type genus Cymbaria has yet to be reported. In addition, the traditional Mongolian medicine Cymbaria daurica is frequently subjected to adulteration or substitution because of the minor morphological differences with Cymbaria mongolica. In this study, the complete chloroplast genomes of the two Cymbaria species were assembled and annotated, and those of other published 52 Orobanchaceae species were retrieved for comparative analyses. We found that the Cymbaria chloroplast genomes are characterized by pseudogenization or loss of stress‐relevant genes (ndh) and a unique rbcL–matK inversion. Unlike the high variability observed in holoparasites, Cymbaria and other hemiparasites exhibit high similarity to autotrophs in genome size, guanine–cytosine (GC) content, and intact genes. Notably, four pairs of specific DNA barcodes were developed and validated to distinguish the medicinal herb from its adulterants. Phylogenetic analyses revealed that the genus Cymbaria and the Schwalbea–Siphonostegia clade are grouped into the tribe Cymbarieae, which forms a sister clade to the remaining Orobanchaceae parasitic lineages. Moreover, the diversification of monophyletic Cymbaria occurred during the late Miocene (6.72 Mya) in the Mongol–Chinese steppe region. Our findings provide valuable genetic resources for studying the phylogeny of Orobanchaceae and plant parasitism, and genetic tools to validate the authenticity of the traditional Mongolian medicine “Xinba.”

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Cymbaria daurica L.: A Mongolian herbal medicine for treating eczema via natural killer cell-mediated cytotoxicity pathway

Cited by 5 publications

References 53 publications

Evaluation of Antioxidant Activity and Treatment of Eczema by Berberine Hydrochloride-Loaded Liposomes-in-Gel

Evaluation of Antioxidant Activity and Treatment of Eczema by Berberine Hydrochloride-Loaded Liposomes-in-Gel

Cord Blood as a Corrector of Hematological and Cytomorphological Characteristics of Lymphohemopoietic Organs in Experimental Atopic Dermatitis

Complete chloroplast genomes of the hemiparasitic genus Cymbaria: Insights into comparative analysis, development of molecular markers, and phylogenetic relationships

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