CYP1B1 expression in ovarian cancer in the laying hen Gallusdomesticus

Zhuge, Yan; Lagman, Jo Ann J.; Ansenberger, Kristine; Mahon, Cassandra; Daikoku, Takiko; Dey, Sudhansu K.; Bahr, Janice M.; Hales, Dale B.

doi:10.1016/j.ygyno.2008.09.026

Cited by 34 publications

(26 citation statements)

References 33 publications

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“…[17, 26, 30, 31, 33], but it is important to determine the precise pathways targeted by the biologically active components of flaxseed that affect the expression of a wide range of cancer targets.…”

Section: Introductionmentioning

confidence: 99%

Flaxseed and its components differentially affect estrogen targets in pre-neoplastic hen ovaries

Dikshit

Gao

Small

et al. 2016

The Journal of Steroid Biochemistry and Molecular Biology

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Flaxseed has been studied for decades for its health benefits that include anti-cancer, cardioprotective, anti-diabetic, anti-inflammatory properties. The biologically active components that mediate these effects are the omega-3 fatty acids and the lignan, secoisolaricirescinol diglucoside. We have previously shown that whole flaxseed supplemented diet decreases the severity and incidence of ovarian cancer while a 15% dose of flaxseed is most protective against inflammation and estrogen-induced chemical and genotoxicity. The objective of this study was to dissect the independent effects of the two flaxseed components on estrogen signaling and metabolism. Two and half year old hens were fed either a control diet, 15% whole flaxseed diet, defatted flax meal diet or 5% flax oil diet for 3 months after which the animals were sacrificed and blood and tissues were harvested. Whole flaxseed diet caused a decrease in expression of ERα. ERα target gene expression was assessed using RT2 profiler PCR array. Some targets involved in the IGF/insulin signaling pathway (IRS1, IGFBP4, IGFBP5) were downregulated by flaxseed and its components. Flaxseed diet also downregulated AKT expression. A number of targets related to NF-κB signaling were altered by flaxseed diet including a series of targets implicated in cancer. Whole flaxseed diet also affected E2 metabolism by increasing CYP1A1 expression with a corresponding increase in the onco-protective E2 metabolite, 2-methoxyestradiol. The weak anti-estrogens, enterolactone, enterodiol and 2-methoxyestradiol, might be working synergistically to generate a protective effect on the ovaries from hens on whole flaxseed diet by altering the estrogen signaling and metabolism.

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Section: Introductionmentioning

confidence: 99%

Flaxseed and its components differentially affect estrogen targets in pre-neoplastic hen ovaries

Dikshit

Gao

Small

et al. 2016

The Journal of Steroid Biochemistry and Molecular Biology

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“…Previous studies have shown that the prevalence of delirium at the time of admission to an acute palliative care unit ranged from 28% to 42% [12,13]. The wide variation in the prevalence of delirium may be attributed to failure of recognition by the medical team and underuse of validated tools to screen for at-risk patients [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…The wide variation in the prevalence of delirium may be attributed to failure of recognition by the medical team and underuse of validated tools to screen for at-risk patients [14,15]. Despite the implications of the presence of delirium on prognosis, few published studies are available on the frequency, course, and outcomes of standardized management of delirium in advanced cancer patients [2,13,16]. In this study, we examined the frequency, characteristics, and outcomes of delirium in patients with advanced cancer who were admitted to an APCU.This knowledge would assist clinicians in improving care for patients with advanced cancer.…”

Section: Introductionmentioning

confidence: 99%

The Frequency, Characteristics, and Outcomes Among Cancer Patients With Delirium Admitted to an Acute Palliative Care Unit

et al. 2015

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Background. Delirium is a common neuropsychiatric condition seen in patients with severe illness, such as advanced cancer. Few published studies are available of the frequency, course, and outcomes of standardized management of delirium in advanced cancer patients admitted to acute palliative care unit (APCU). In this study, we examined the frequency, characteristics, and outcomes of delirium in patients with advanced cancer admitted to an APCU. Methods. Medical records of 609 consecutive patients admitted to the APCU from January 2011 through December 2011 were reviewed. Data on patients’ demographics; Memorial Delirium Assessment Scale (MDAS) score; palliative care specialist (PCS) diagnosis of delirium; delirium etiology, subtype, and reversibility; late development of delirium; and discharge outcome were collected. Delirium was diagnosed with MDAS score ≥7 and by a PCS using Diagnostic and Statistical Manual, 4th edition, Text Revision criteria. All patients admitted to the APCU received standardized assessments and management of delirium per best practice guidelines in delirium management. Results. Of 556 patients in the APCU, 323 (58%) had a diagnosis of delirium. Of these, 229 (71%) had a delirium diagnosis on admission and 94 (29%) developed delirium after admission to the APCU. Delirium reversed in 85 of 323 episodes (26%). Half of patients with delirium (n = 162) died. Patients with the diagnosis of delirium had a lower median overall survival than those without delirium. Patients who developed delirium after admission to the APCU had poorer survival (p ≤ .0001) and a lower rate of delirium reversal (p = .03) compared with those admitted with delirium. Conclusion. More than half of the patients admitted to the APCU had delirium. Reversibility occurred in almost one‐third of cases. Diagnosis of delirium was associated with poorer survival. Implications for Practice: Delirium is the most common neuropsychiatric condition in patients with severe medical illness and those at the end of life. It can be a source of distress for patients, their families, and the medical team. When missed, or if symptoms are misinterpreted, delirium may also lead to unnecessary interventions. This underlines the importance of diagnosis and detection of delirium in populations that are at increased risk. This study has important implications in practice, as it can assist clinicians in making decisions regarding other medical interventions, advance care planning, and communicating with families relating to end‐of‐life issues.

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“…A remarkable example of the use of an animal model as a research tool is the utilization of the domestic hen (Gallus gallus domesticus) to study the molecular mechanisms involved in the development of ovarian carcinoma in humans [1][2][3][4][5][6]. Similar to what occurs in ovarian carcinoma, leiomyoma-like masses also occur on the oviduct of commercial strains of chickens [7,8].…”

Section: Introductionmentioning

confidence: 99%

Validation of the Aging Hen (Gallus gallus domesticus) as an Animal Model for Uterine Leiomyomas1

Machado

Bahr

Hales

et al. 2012

Biology of Reproduction

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Uterine leiomyomas, or fibroids, are the most frequent gynecological tumors in premenopausal women with as many as 65% of women becoming clinically symptomatic. Uterine fibroids are benign myometrial tumors that produce large quantities of extracellular matrix proteins. Despite its high morbidity, the molecular basis underlying the development of uterine leiomyomas is not well understood. Domestic hens of Gallus gallus domesticus develop oviductal leiomyomas similar to those found in humans. We investigated the natural history of chicken leiomyomas, in vivo expression of protein biomarkers, and in vitro expression of ovarian steroid receptors. Based on the analysis of 263 hens, tumor prevalence, tumor number per hen, and tumor size increased as the hens aged. Immunohistochemistry for alpha-smooth muscle actin (SMA) and desmin confirmed the smooth muscle phenotype of the chicken leiomyomas. Intense collagen expression was detected in these oviductal leiomyomas by Mason's trichrome, and the tumors also showed increased expression of TGFB3 and collagen type I mRNAs. Consistent with human leiomyomas, chicken fibroids displayed increased BCL2 and estrogen (E) and progesterone (P) receptor expression. Chicken leiomyomas were dissociated for in vitro culture. Cells from explants were positive for SMA, desmin, and E and P receptors until the fourth passage. These cells also displayed a response similar to human cells when challenged with halofuginone, an antifibrotic agent. Our findings indicate that the chicken is an excellent complementary model for studies involving the pathophysiology of human uterine leiomyomas.

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CYP1B1 expression in ovarian cancer in the laying hen Gallusdomesticus

Cited by 34 publications

References 33 publications

Flaxseed and its components differentially affect estrogen targets in pre-neoplastic hen ovaries

Flaxseed and its components differentially affect estrogen targets in pre-neoplastic hen ovaries

The Frequency, Characteristics, and Outcomes Among Cancer Patients With Delirium Admitted to an Acute Palliative Care Unit

Validation of the Aging Hen (Gallus gallus domesticus) as an Animal Model for Uterine Leiomyomas1

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