CYP2E1 changes the biological function of gastric cancer cells via the PI3K/Akt/mTOR signaling pathway

Abstract

The present study investigated the role of cytochrome P450 family 2 subfamily e polypeptide 1 (cYP2e1) in the development and progression of gastric cancer (Gc). The expression levels of cYP2e1 in MGc-803 Gc cells and normal GeS-1 cells were investigated via western blotting, and it was identified that the expression of CYP2E1 was different between GeS-1 and MGc-803 cells. cYP2e1 was overexpressed in MGc-803 cells using a lentiviral vector GV358. cell counting Kit-8, flow cytometry, cell migration and Matrigel invasion assays suggested that overexpression of cYP2e1 promoted the proliferation and invasion, and inhibited the apoptosis of Gc cells. The relationship between cYP2e1 expression and key signaling molecules in the Pi3K/akt/mTor signaling pathway was assessed. reverse transcription-quantitative Pcr analysis showed that mTor mrna expression was significantly increased after overexpression of cYP2e1 (P<0.05). Western blotting results showed that overexpression of cYP2e1 upregulated the expression of phosphorylated (p)-akt, p-mTor and p-p70 ribosomal protein S6 kinase (P70S6K; Ser371) proteins (P<0.05). To further investigate the relationship between cYP2e1 and the Pi3K/akt/mTor signaling pathway in Gc cells, MGc-803 cells were treated with the Pi3K inhibitor lY294002, and changes in the expression levels of Pi3K, aKT, mTor, P70S6K and cYP2e1 were observed. The present results showed that lY294002 downregulated the expression of Pi3K, cYP2e1, aKT, mTor and P70S6K (P<0.05). Therefore, changes in the biological function of Gc cells induced by cYP2e1 overexpression may be via the Pi3K/akt/mTor signaling pathway.

Curcumin Inhibits the Proliferation of Renal Cancer 786-O Cells through MTOR Signaling Pathway and Its Mechanism

“…In recent years, the activation of MTOR signaling pathway is related to the occurrence of various malignant tumors. It has become a new idea for cancer treatment to block the activation of downstream signaling molecules by using signaling pathway blockers to inhibit cancer cells growth [ 19 – 21 ]. In this study, curcumin was used to explore the effect and the possible molecular mechanism on proliferation, migration, invasion, and apoptosis of 786-O cell by blocking MTOR signaling pathway, thus providing theoretical basis for clinical drug treatment of renal cancer.…”

DriveWays: a method for identifying possibly overlapping driver pathways in cancer

“…The module itself has the best match with the MAPK signaling pathway . Further support of the DriveWays’ choice of FLNA as a candidate driver is found in various recent studies demonstrating its role in tumor progression in a wide range of cancer types 51 – 53 . Repeating the same analysis under the breast cancer setting we find that PIK3R1, CDK4, CDK6, and SF3B1 satisfy the criteria for driver candidacy, that is each one appears among the top twenty DriveWays modules, does not exist in the reference gene set CMbreast , but does exist in the KEGG pathway corresponding to the best match of its module.…”

Bioinformatics analysis of aberrantly methylated- differentially expressed genes in gastric cancer

“…promotes the proliferation and invasion of GC, and inhibits their apoptosis [39]. Studies have proved the dose-dependent enhancement of salt in the gastric chemical carcinogenesis of Mongolian gerbils infected with Helicobacter pylori (HP).…”