CYP4CL2 Confers Metabolic Resistance to Pyridaben in the Citrus Pest Mite <i>Panonychus citri</i>

Pan, Deng; Xia, Menghao; Li, Chuanzhen; Liu, Xunyan; Archdeacon, Lewis; O’Reilly, Andrias O.; Yuan, Guorui; Wang, Jinjun; Dou, Wei

doi:10.1021/acs.jafc.3c06921

Cited by 7 publications

(2 citation statements)

References 50 publications

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“…The resistance of pyridaben was decreased after silencing CYP4CL2 in P. citri . In Zeugodacus cucurbitae, the susceptibility to β-cypermethrin was increased after silencing ZcGSTe4 .…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Characterization of Carboxylesterase Genes Involved in Spirodiclofen Resistance in Panonychus citri (McGregor)

Yang,

Li,

Cui

et al. 2024

J. Agric. Food Chem.

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Overexpression of carboxyl/cholinesterase (CCE) genes has been reported to be associated with many cases of pesticide resistance in arthropods. However, it has been rarely documented that CCE genes participate in spirodiclofen resistance in Panonychus citri. In previous research, we found that spirodiclofen resistance is related to increased P450 and CCE enzyme activities in P. citri. In this study, we identified two CCE genes, PcCCE3 and PcCCE5, which were significantly upregulated in spirodiclofenresistant strain and after exposure to spirodiclofen. RNA interference of PcCCE3 and PcCCE5 increased the spirodiclofen susceptibility in P. citri. In vitro metabolism indicated that PcCCE3 and PcCCE5 could interact with spirodiclofen, but metabolites were detected only in the PcCCE3 treatment. Our results indicated that PcCCE3 participates in spirodiclofen resistance through direct metabolism, and PcCCE5 may be involved in the spirodiclofen resistance by passive binding and sequestration, which provides new insights into spirodiclofen resistance in P. citri.

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“…The resistance of pyridaben was decreased after silencing CYP4CL2 in P. citri . In Zeugodacus cucurbitae, the susceptibility to β-cypermethrin was increased after silencing ZcGSTe4 .…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Characterization of Carboxylesterase Genes Involved in Spirodiclofen Resistance in Panonychus citri (McGregor)

Yang,

Li,

Cui

et al. 2024

J. Agric. Food Chem.

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“…cinnabarinus) and Panonychus citri (P. citri), which are widely distributed polyphagous pests in agricultural areas, have developed resistance due to their strong adaptability, rapid reproduction, and the often unreasonable use of existing acaricides. , Consequently, there is a significant demand for developing acaricides with novel molecular scaffolds and unique mechanisms of action. Particularly, the isoindolinone scaffolds represent a growing class of important bioactive natural products and pharmaceuticals (Figure ).…”

Section: Introductionmentioning

confidence: 99%

Unveiling FM-1088: A Breakthrough in Isoindolinone-Based Acaricide Development

Liu,

Gao,

Pei

et al. 2024

J. Agric. Food Chem.

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The increasing resistance of agricultural pests to existing acaricides presents a significant challenge to sustainable agriculture. Therefore, this study introduced FM-1088, a novel isoindolinone-based phenyl trifluoroethyl thioether derivative generated through an innovative design strategy combining bioisosterism and novel cyclization methods. We synthesized several compounds and evaluated their acaricidal efficacy against Tetranychus cinnabarinus in greenhouses and Panonychus citri in field settings. FM-1088 emerged as a standout candidate, demonstrating a lower median lethal concentration (LC 50 ) of 0.722 mg/L compared to the commercial acaricide, cyetpyrafen. Notably, 30 days after application, FM-1088 showed a field control efficacy of 96.4% against P. citri, highlighting its potential for broader applications. The results underscore the utility of the isoindolinone scaffold in pesticide development, offering a promising solution to combat pest resistance with implications for enhanced crop protection and agricultural productivity. Future studies should explore the detailed mode of action of FM-1088 and its potential applicability across diverse agricultural settings, further confirming its role as a sustainable solution for pest management.

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Synergistic action and mechanism of scoparone, a key bioactive component of Artemisia capillaris, and spirodiclofen against spider mites

Zhou,

Wan,

Lai

et al. 2024

Pest Management Science

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BACKGROUNDPlants have numerous defensive secondary metabolites to withstand insect attacks. Scoparone, which is extracted from the medicinal plant Artemisia capillaris, has potent acaricidal effects on Tetranychus cinnabarinus. Spirodiclofen, derived from a tetronic acid derivative, is a potent commercial acaricide that is extensively used globally. However, whether scoparone has synergistic effects when used in conjunction with spirodiclofen and the underlying synergistic mechanism remains unclear.RESULTSScoparone exhibited a potent synergistic effect when it was combined with spirodiclofen at a 1:9 ratio. Subsequently, cytochrome P450 monooxygenase (P450) activity, RNA‐Seq and qPCR assays indicated that the enzyme activity of P450 and the expression of one P450 gene from T. cinnabarinus, TcCYP388A1, were significantly inhibited by scoparone and spirodiclofen + scoparone; conversely, P450 was activated in spirodiclofen‐exposed mites. Importantly, RNAi‐mediated silencing of the TcCYP388A1 gene markedly increased the susceptibility of spider mites to spirodiclofen, scoparone and spirodiclofen + scoparone, and in vitro, the recombinant TcCYP388A1 protein could metabolize spirodiclofen. Molecular docking and functional analyses further indicated that R117, which is highly conserved in Arachnoidea species, may be a vital specific binding site for scoparone in the mite TcCYP388A1 protein. This binding site was subsequently confirmed using mutagenesis data, which revealed that this binding site was the sole site selected by scoparone in spider mites over mammalian or fly CYP388A1.CONCLUSIONSThese results indicate that the synergistic effects of scoparone and spirodiclofen on mites occurs through the inhibition of P450 activity, thus reducing spirodiclofen metabolism. The synergistic effect of this potent natural product on the detoxification enzyme‐targeted activity of commercial acaricides may offer a sustainable strategy for pest mite resistance management. © 2024 Society of Chemical Industry.

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CYP4CL2 Confers Metabolic Resistance to Pyridaben in the Citrus Pest Mite Panonychus citri

Cited by 7 publications

References 50 publications

Identification and Functional Characterization of Carboxylesterase Genes Involved in Spirodiclofen Resistance in Panonychus citri (McGregor)

Identification and Functional Characterization of Carboxylesterase Genes Involved in Spirodiclofen Resistance in Panonychus citri (McGregor)

Unveiling FM-1088: A Breakthrough in Isoindolinone-Based Acaricide Development

Synergistic action and mechanism of scoparone, a key bioactive component of Artemisia capillaris, and spirodiclofen against spider mites

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