CYP701A26 is characterized as an ent-kaurene oxidase with putative involvement in maize gibberellin biosynthesis

Abstract

CYP701A26 was characterized to exhibit ent-kaurene oxidase activity with substrate promiscuity and might be involved in maize GA biosynthesis, and its homologue CYP701A43 did not show such function and might play roles in stress response.

A dolabralexin-deficient mutant provides insight into specialized diterpenoid metabolism in maize (Zea mays)

“…FPKM (fragments per kilobase of exon per million mapped fragments) values for the genes of interest from all datasets used here are available in Supplemental Table 4. Across all four developmental atlases (Chen et al, 2014; Stelpflug et al, 2016; Kremling et al, 2018; Yi et al, 2019), GA pathway genes, including ZmAn1 , ZmKo1 , ZmKsl3 and to lesser extent ZmKsl5 showed patterns of co-expression across various primarily above-ground tissues and timepoints (Bensen et al, 1995; Fu et al, 2016; Mao et al, 2017), as expected (Fig. 4).…”

“…Two additional substrate-promiscuous P450s, Kaurene Oxidase (KO) 1 (ZmCYP701A26) and ZmKO2 (ZmCYP701A43), are tandemly arrayed on the same chromosome. While ZmKO1 exhibits activity in the GA pathway, ZmKO2 biochemically responsible for highly oxidized KX (Mafu et al, 2016; Mao et al, 2017; Ding et al, 2019).…”

Gibberellin metabolism and signaling and its molecular mechanism in regulating seed germination and dormancy

“…的KO基因, 并发现CYP701A26表现出KO活性的底物 复杂性 [35] , 而在大麦中只鉴定出1个KAO基因; 豌豆 (Pisum sativum)拥有2个KAO基因, 一个具有通常的 KAO活性, 另一个仅在发育的种子中表达 [36] . [30] .…”

Discovery, Biosynthesis and Stress-Related Accumulation of Dolabradiene-Derived Defenses in Maize

“…Comparatively, less was known about the complexity of maize diterpenoid biosynthesis. Characterized maize diterpenoid enzymes include the ENT-COPALYL DIPHOSPHATE (CPP) SYNTHASE ZmAN1 (Bensen et al, 1995), the ENT-KAURENE SYNTHASES ZmTPS1, ZmKLS3, and ZmKSL5 (Fu et al, 2016), and the ENT-KAURENE OXIDASE CYP701A26 (Mao et al, 2017;Fig. 1).…”