Cypermethrin induces apoptosis, autophagy and inflammation via ERS-ROS-NF-κB axis in hepatocytes of carp (Cyprinus carpio)

Li, Di; Zhang, Kaixuan; Xu, Chenchen; Jiang, Yangyang; Shan, Jianhua; Zhang, Ziwei; Cai, Jingzeng

doi:10.1016/j.pestbp.2023.105625

Cited by 12 publications

(4 citation statements)

References 52 publications

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“…The operational procedure is executed in strict accordance with the guidelines provided by the commercial AO/EB staining kit (Beyotime Biotechnology, China). Briefly, after cells were treated differently, AO/EB working solution (10 μL/mL) was added for staining for 5 min . Finally, fluorescence images were collected by a fluorescent inverted microscope (Olympus, IX53, Japan).…”

Section: Methodsmentioning

confidence: 99%

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New Insights into Decabromodiphenyl Ether-Induced Splenic Injury in Chickens: Involvement of ROS-Mediated Endoplasmic Reticulum Stress Pathway Triggering Autophagy and Apoptosis

Shi,

Dong,

Shan

et al. 2024

J. Agric. Food Chem.

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Decabromodiphenyl ether (BDE-209) is a widely used brominated flame retardant that can easily detach from materials and enter into feed and foodstuffs, posing a serious risk to human and animal health and food safety of animal origin. However, the immunotoxic effects of BDE-209 on the avian spleen and the exact mechanism of the toxicity remain unknown. Therefore, we established an experimental model of BDE-209-exposed chickens and a positive control model of cyclophosphamideinduced immunosuppression in vivo and treated MDCC-MSB-1 cells and chicken splenic primary lymphocytes with BDE-209 in vitro. The results showed that BDE-209 treatment caused morphological and structural abnormalities in the chicken spleens. Mechanistically, indicators related to oxidative stress, endoplasmic reticulum stress (ERS), autophagy, and apoptosis were significantly altered by BDE-209 exposure in both the spleen and lymphocytes, but the use of the N-acetylcysteine or the 4phenylbutyric acid significantly reversed these changes. In addition, BDE-209 exposure decreased the spleen antimicrobial peptide and immunoglobulin gene expression. In conclusion, the present research revealed that BDE-209 exposure enhanced lymphocyte autophagy and apoptosis in chicken spleen via the ROS-mediated ERS pathway. This signaling cascade regulatory relationship not only opens up a new avenue for studying BDE-209 immunotoxicity but also provides important insights into preventing BDE-209 hazards to animal health.

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Section: Methodsmentioning

confidence: 99%

“…Briefly, after cells were treated differently, AO/EB working solution (10 μL/mL) was added for staining for 5 min. 40 Finally, fluorescence images were collected by a fluorescent inverted microscope (Olympus, IX53, Japan).…”

Section: Acridine Orange/ethidium Bromide (Ao/eb) Dual Stainingmentioning

confidence: 99%

New Insights into Decabromodiphenyl Ether-Induced Splenic Injury in Chickens: Involvement of ROS-Mediated Endoplasmic Reticulum Stress Pathway Triggering Autophagy and Apoptosis

Shi,

Dong,

Shan

et al. 2024

J. Agric. Food Chem.

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“…Therefore, evaluating the effects of environmental pollutants on liver tissue serves as a viable method for assessing the safety of the aquatic environment [16,17]. Additionally, the growing concerns surrounding the harmful effects and toxic mechanisms of NIC on black carp liver highlight the need for further investigation.…”

Section: Introductionmentioning

confidence: 99%

Investigating Immunotoxicity in Black Carp (Mylopharyngodon piceus) Fingerlings Exposed to Niclosamide

Wu,

Yuan,

Tian

et al. 2024

Life

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Niclosamide (NIC) is a potent salicylanilide molluscicide/helminthicide commonly utilized for parasite and mollusc control in aquatic environments. Due to its persistent presence in water bodies, there is growing concern regarding its impact on aquatic organisms, yet this remains inadequately elucidated. Consequently, this study aims to assess the hepatotoxic effects and detoxification capacity of black carp (Mylopharyngodon piceus) in a semi-static system, employing various parameters for analysis. NIC was applied to juvenile black carp at three different concentrations (0, 10 and 50 μg/L) for 28 days in an environmentally realistic manner. Exposure to 50 μg/L NIC resulted in an increase in hepatic lysozyme (LYZ), alkaline phosphatase (ALP), and complement 4 (C4) levels while simultaneously causing a decrease in peroxidase (POD) activity. Additionally, NIC exposure exhibited a dose-dependent effect on elevating serum levels of LYZ, ALP, complement 3 (C3), C4, and immunoglobulin T (IgT). Notably, the mRNA levels of immune-related genes tnfα, il8, and il6, as well as nramp and leap2, were upregulated in fish exposed to NIC. RNA-Seq analysis identified 219 differentially expressed genes (DEGs) in M. piceus after NIC exposure, with 94 upregulated and 125 downregulated genes. KEGG and GO analyses showed enrichment in drug metabolism pathways and activities related to oxidoreductase, lip oprotein particles, and cholesterol transport at 50 μg/L NIC. Additionally, numerous genes associated with lipid metabolism, oxidative stress, and innate immunity were upregulated in NIC-exposed M. piceus. Taken together, these findings indicate that NIC has the potential to cause hepatotoxicity and immunotoxicity in M. piceus. This research offers important insights for further understanding the impact of molluscicide/helminthicide aquatic toxicity in ecosystems.

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“…Ullah et al 2018;Li et al 2023). Several previous investigations have con rmed the impact of cypermethrin on sh under sublethal concentrations(Carriquiriborde et al 2009), with documented effects on sh reproduction and growth.…”

mentioning

confidence: 97%

Cypermethrin pesticide-induced effects in carp fish (Cirrhinus mrigala): acute and chronic toxicity

Ray,

Kundu,

Salauddin

et al. 2023

Preprint

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Pesticide pollution in aquatic ecosystems poses a prevalent and pressing concern, with a particular emphasis on developing nations. The effect of cypermethrin (CP), a synthetic type II pyrethroid pesticide, on phenotypic traits, enzyme activities, and biochemical nutrients in freshwater, mrigal fish (Cirrhinus mrigala) was investigated in this study. Fingerlings were exposed to CP concentrations of 0.0065, 0.013, and 0.026 ppm to determine the median lethal concentration (LC50) for 32 hours and subsequently, three sub-lethal concentrations of 0.0026 ppm, 0.0013 ppm, and 0.00065 ppm were employed to analyze the chronic toxicity stress for 6 weeks. Fish fingerlings tolerated well at lower concentrations (0.0065 ppm) of CP over exposure times, while 50% mortality occurred at 0.13 ppm and 0.026 ppm after 32 hours and 4 hours of exposure, respectively. In the present investigation, growth and eye diameter showed marked differences among treatments, while remaining, total length (TL), standard length (SL), head length (HL), tail length, head width (HW), body depth (BD), body area (BA) and dressing percentage (DP) were found similar among treatments. This study revealed that the amylase and protease activities decreased significantly at increasing concentrations of CP. On the other hand, biochemical nutrients (protein, lipid, moisture, and ash) among the treatments were similar. This study proves that short-time exposure to CP has a negative impact on fish growth and enzymatic activities, however, further studies, such as metabolomic approaches are required to fully explore the effect of CP during sub-lethal exposures.

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Cypermethrin induces apoptosis, autophagy and inflammation via ERS-ROS-NF-κB axis in hepatocytes of carp (Cyprinus carpio)

Cited by 12 publications

References 52 publications

New Insights into Decabromodiphenyl Ether-Induced Splenic Injury in Chickens: Involvement of ROS-Mediated Endoplasmic Reticulum Stress Pathway Triggering Autophagy and Apoptosis

New Insights into Decabromodiphenyl Ether-Induced Splenic Injury in Chickens: Involvement of ROS-Mediated Endoplasmic Reticulum Stress Pathway Triggering Autophagy and Apoptosis

Investigating Immunotoxicity in Black Carp (Mylopharyngodon piceus) Fingerlings Exposed to Niclosamide

Cypermethrin pesticide-induced effects in carp fish (Cirrhinus mrigala): acute and chronic toxicity

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