Cyr61 increases migration and MMP-13 expression via αvβ3 integrin, FAK, ERK and AP-1-dependent pathway in human chondrosarcoma cells

Tan, Tzu Wei; Yang, Wei; Lin, Yexin; Hsu, Sheng Feng; Li, Te Mao; Kao, Shung Te; Chen, Wen Chi; Fong, Yi Chin; Tang, Chih Hsin

doi:10.1093/carcin/bgn284

Cited by 69 publications

(76 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Involvement of MMP-13 in the IL-6-directed Cell Migration of Chondrosarcoma-Previous studies have shown a significant expression of MMP-1, -2, -3, -9, and -13 in human chondrosarcoma cells (7,8). We therefore hypothesized that any of these MMPs may be involved in IL-6-directed chondrosarcoma migration.…”

Section: Il-6-directed Chondrosarcoma Cell Migration Via the Il-6mentioning

confidence: 94%

“…1, A and C, lanes 1-4) were significantly higher than in normal cartilage (Fig. 1, A and C, lanes [5][6][7][8][9]. The qualification data of IL-6 protein expression are shown in Fig.…”

Section: Il-6-directed Chondrosarcoma Cell Migration Via the Il-6mentioning

confidence: 97%

“…MMPs, cytokines, growth factors, and chemokines have been shown to regulate tumor cell invasion through autocrine or paracrine pathways (4). Previous studies demonstrated the expression of MMP-1, -2, -3, -9, and MMP-13 in human chondrosarcoma cells (7,8). MMP-13 also mediates the chemokine-induced migration of chondrosarcoma (8, 9).…”

mentioning

confidence: 98%

See 2 more Smart Citations

IL-6 Increases MMP-13 Expression and Motility in Human Chondrosarcoma Cells

Tang

Chen²,

Chen

et al. 2011

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. IL-6 is a multifunctional cytokine that is associated with the disease status and outcomes of cancers. However, the effect of IL-6 on the migration activity of human chondrosarcoma cells is mostly unknown. Here, we found that IL-6 increased the migration and expression of MMP-13 in human chondrosarcoma cells. We also found that human chondrosarcoma tissues had significant expression of IL-6, which was higher than that in normal cartilage. IL-6-mediated migration and MMP-13 up-regulation were attenuated by anti-IL-6 receptor antibody, Ras, Raf-1, and a MEK inhibitor. Activation of the Ras, Raf-1, MEK, ERK, and NF-B signaling pathways after IL-6 treatment was demonstrated, and IL-6-induced MMP-13 expression and migration activity were inhibited by the specific inhibitor and mutant Ras, Raf-1, MEK, ERK, and NF-B cascades. In addition, migration-prone sublines demonstrated that cells with increasing migration ability had greater expression of IL-6 and MMP-13. Taken together, these results indicate that IL-6 and IL-6 receptor interaction enhances migration of chondrosarcoma through an increase in MMP-13 production.Chondrosarcoma is the second most common malignancy of bone, and it has a poor response to the chemotherapy or radiation treatment currently used, making the management of chondrosarcomas a complicated challenge (1). Clinically, surgical resection remains the primary mode of therapy for chondrosarcoma. In the absence of an effective adjuvant therapy, this mesenchymal malignancy has a poor prognosis, and therefore, it is important to explore novel and adequate remedies (2). Because chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and metastasize distantly (2), an approach that decreases its ability to invade and metastasize may facilitate the development of effective adjuvant therapy.The tumor metastatic cascade consists of multiple successive steps, including adhesion of tumor cells at the primary site, invasion into the intravascular space, dissemination to distant sites, adhesion of tumor cells to the vascular endothelium of distant tissues, extravasation and invasion into surrounding tissues, and finally formation of secondary tumor colonies (3). To facilitate the cell motility, invading cells need to change the cell-cell adhesion properties, rearrange the extracellular matrix environment, suppress anoikis, and reorganize their cytoskeletons (4). Matrix metalloproteinases (MMPs) 3 have important roles in these processes because their proteolytic activities assist in degradation of the extracellular matrix and basement membrane (5, 6). MMPs, cytokines, growth factors, and chemokines have been shown to regulate tumor cell invasion through autocrine or paracrine pathways (4). Previous studies demonstrated the expression of MMP-1, -2, -3, -9, and MMP-13 in human chondrosarcoma cel...

show abstract

Section: Il-6-directed Chondrosarcoma Cell Migration Via the Il-6mentioning

confidence: 94%

“…1, A and C, lanes 1-4) were significantly higher than in normal cartilage (Fig. 1, A and C, lanes [5][6][7][8][9]. The qualification data of IL-6 protein expression are shown in Fig.…”

Section: Il-6-directed Chondrosarcoma Cell Migration Via the Il-6mentioning

confidence: 97%

See 1 more Smart Citation

IL-6 Increases MMP-13 Expression and Motility in Human Chondrosarcoma Cells

Tang

Chen²,

Chen

et al. 2011

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although HB-EGF and Cyr61 act through different mechanisms, being, respectively, a growth factor and an extracellular adhesion substrate, these molecules share common features, since both are intracellularly regulated via AP-1 (31)(32)(33)(34). It has been reported that the increased AP-1 activation in rat kidney epithelial cells (32) and in bladder smooth muscle cells is able to induce HB-EGF expression (31).…”

Section: Discussionmentioning

confidence: 99%

“…Addition of recombinant Cyr61 increases the migration as well as the invasive properties of trophoblast cells (30), supporting its beneficial role on trophoblast invasiveness. Both these molecules share a common intracellular pathway, the activator protein 1 (AP-1), a transcription factor recognizing specific binding sites in the promoter region of HB-EGF and Cyr61 (31)(32)(33)(34).…”

mentioning

confidence: 99%

Emerging nonanticoagulant role of low molecular weight heparins on extravillous trophoblast functions and on heparin binding–epidermal growth factor and cystein-rich angiogenic inducer 61 expression

D’Ippolito

Nicuolo

Marana

et al. 2012

Fertility and Sterility

View full text Add to dashboard Cite

A novel hydroxyproline rich glycopeptide from pericarp of Datura stramonium: Proficiently eradicate the biofilm of antifungals resistant Candida albicans

Mandal

2012

Biopolymers

View full text Add to dashboard Cite

The increasing threats of multidrug resistant fungal pathogens, several studies have been focused to identify novel antifungal plant peptides with unique characteristics. Plants have been defending themselves against fungal infection by generating effective antifungal molecules. Here, a novel antifungal peptide with molecular mass of 4.0 kDa was purified from the pericarp of D. stramonium using reversed phase chromatography system after acetic acid extraction. Presence of sugar moieties (-GlcNAc-) in the peptide have been confirmed using thin layer chromatographic (TLC), CD polarimeter, and MALDI MS analysis. Complete amino acid sequences of this peptide by PSD MALDI MS analysis revealed to contain hydroxyproline in the centre of two cysteine residues. After sequencing "TFPKCAPTRhyPhy PGPKhyPCDINNFKSKFWHIWRA-(GlcNAc-)Asn" peptide named as "datucin." Antifungal sensitivity of datucin have been performed for both planktonic cells and biofilm phenotype ofa multidrug resistant clinical isolates, C. albicans and showed a MIC and MBEC values of 1 microM and 2 microM, respectively. Hence, D. stramonium offers a potential source of novel antifungal peptide datucin with possible utility in antifungal chemotherapy.

show abstract

Cyr61 increases migration and MMP-13 expression via αvβ3 integrin, FAK, ERK and AP-1-dependent pathway in human chondrosarcoma cells

Cited by 69 publications

References 44 publications

IL-6 Increases MMP-13 Expression and Motility in Human Chondrosarcoma Cells

IL-6 Increases MMP-13 Expression and Motility in Human Chondrosarcoma Cells

Emerging nonanticoagulant role of low molecular weight heparins on extravillous trophoblast functions and on heparin binding–epidermal growth factor and cystein-rich angiogenic inducer 61 expression

A novel hydroxyproline rich glycopeptide from pericarp of Datura stramonium: Proficiently eradicate the biofilm of antifungals resistant Candida albicans

Contact Info

Product

Resources

About