Cyst fluid iron-related compounds as useful markers to distinguish malignant transformation from benign endometriotic cysts

Yoshimoto, Chiharu; Iwabuchi, Takuya; Shinmoto, Hiroshi; Kobayashi, Hiroshi

doi:10.3233/cbm-150484

Cited by 39 publications

(69 citation statements)

References 15 publications

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“…The Δl values and total Hb concentrations in 46 samples exhibited an exponential correlation (r=0.558), suggesting that the Δl value may reflect the Hb concentration. The present results were in agreement with those of Yoshimoto et al (6), who reported the cystic fluid concentration of total iron, heme iron, free iron and Hb species (6,7). Previous studies of Hb species have reported differences between OE and EAOC samples (8).…”

Section: Discussionsupporting

confidence: 83%

“…Furthermore, the cystic fluid Hb concentrations were reduced in patients with EAOC (6)(7)(8). The Δl values and total Hb concentrations in 46 samples exhibited an exponential correlation (r=0.558), suggesting that the Δl value may reflect the Hb concentration.…”

Section: Discussionmentioning

confidence: 84%

“…Cystic fluid total Hb concentrations were measured as described previously (6,8,13). From the correlation data, a formula was calculated to convert heme levels (mg/l) to hemoglobin (g/dl).…”

Section: Methodsmentioning

confidence: 99%

“…Recent progress in research on the pathogenesis of EAOC is based on key developments in two areas: i) New mechanistic concepts regarding the pathogenesis of EAOC revealed a key role of hemoglobin (Hb), heme and iron-induced oxidative stress in the OE cystic fluid, wherein an imbalance between the overproduction of iron-induced oxidative stress and defense mechanisms could trigger DNA damage and carcinogenesis; and ii) the establishment of novel approaches to identify stress biomarkers, such as Hb and iron, for the prediction of malignant transformation (2,6,7). A recent ex vivo study revealed that electronic absorption spectroscopy using visible light at 580 and 620 nm provides a measure of Hb species in endometriotic cystic fluid by monitoring the relative concentrations of oxyhemoglobin and methemoglobin, respectively (8).…”

Section: Introductionmentioning

confidence: 99%

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Discrimination of malignant transformation from benign endometriosis using a near‑infrared approach

et al. 2018

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Abstract. The aim of the present single-center retrospective study was to investigate the discrimination of malignant transformation from ovarian endometrioma (OE) using a near-infrared approach ex vivo. Cystic fluid samples were collected from patients with OE (n=34) and endometriosis-associated ovarian cancer (EAOC) (n=12). The light reflected from each sample of cystic fluid [change in luminance, Δl (cd/m 2 ) = background luminance-cystic fluid luminance at 800 nm] was spectrally measured by a near-infrared CCD camera with band-path filter (800 nm). The Δl in EAOC was significantly lower compared with that in OE. On regression analysis, a positive correlation was observed between the Δl and Hb level in the cystic fluid, and this association was exponential. The diagnostic sensitivity and specificity of Δl was 83.3 and 94.1% at the cutoff value of 21.5 cd/m 2 , with an area under the ROC curve of 0.897. The present ex vivo study potentially provides a powerful near-infrared approach for quantitative discrimination between EAOC and benign OE, with high sensitivity and specificity, which may have clinical applications.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 84%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Discrimination of malignant transformation from benign endometriosis using a near‑infrared approach

et al. 2018

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“…TAC/Heme-iron ref lects the antioxidant capacity of water-soluble molecules, since the majority of iron exists as heme-iron in OE and EAOC cyst fluid samples (9).…”

Section: Introductionmentioning

confidence: 99%

Comparison of redox parameters in ovarian endometrioma and its malignant transformation

et al. 2018

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The present study aimed to evaluate the levels of oxidative stress and antioxidant markers in benign endometrioma (OE) and its malignant transformation [endometriosis-associated ovarian cancer (EAOC)] by measuring 8-hydroxy-2-deoxyguanosine (8-OHdG), heme oxygenase-1 (HO-1) and total antioxidant capacity (TAC/Heme-iron) alterations associated with disease progression. Cyst fluid samples from 44 patients with OE and 14 patients with EAOC were studied using an enzyme-linked immunosorbent assay. A χ test, t-test and Pearson correlation test were performed using SPSS version 22.0. The cut-off point, sensitivity and specificity of each marker for EAOC diagnosis were evaluated by receiver operating characteristic curve analysis. Cyst fluid 8-OHdG and HO-1 levels in the EAOC group were significantly decreased compared with the OE subjects (P=0.013 and P<0.001, respectively). The levels of TAC/Heme-iron in patients with EAOC were significantly higher compared with those in the OE subjects (P<0.001). The present study demonstrated a positive correlation between 8-OHdG and HO-1 levels (P=0.012). HO-1 exhibited the highest discriminant value for EAOC (Area Under the Curve=0.901). The optimal cut-off point of HO-1 for the diagnosis of EAOC was 2.314 ng/ml, with a sensitivity and specifity of 95.2 and 85.7%, respectively. The present study revealed a clear separation between the overall redox state in OE and EAOC. It was concluded that characteristic alterations in important factors in redox may be helpful for understanding the pathogenesis of the malignant transformation of endometriosis.

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Activation of cGAS/STING Drives Inflammation and Cellular Senescence of Macrophages in Ovarian Endometrioma Induced by Endometriotic Cyst Fluid

Ye,

Sun,

Cai

et al. 2024

Advanced Biology

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Ovarian endometrioma (OE) is a common gynecological condition characterized by the formation of “chocolate cysts”. Recent research indicates that the cyst fluid acts as a “toxic environment” for the ovary and plays a significant role in the development of OE, with macrophages being pivotal. However, the specific molecular and cellular mechanisms of it are not fully understood. In this study, clinical samples are integrated, single‐cell sequencing, in vivo and in vitro experimental models to comprehensively investigate the effects of OE fluid on ovarian function and the mechanisms of it. Combined with bioinformatics analysis and experimental validation, the findings demonstrate that OE fluid can cause ovarian function decline, which associated with inflammatory response, and mitochondrial dysfunction and cellular senescence, while activating the cGAS/STING signaling pathway. As a STING inhibitor, H‐151 effectively alleviates ovarian dysfunction, inflammatory state and cell apoptosis induced by OE fluid. Furthermore, it is also discovered that H‐151 can inhibit OE fluid‐induced mitochondrial dysfunction and cellular senescence. These findings provide important theoretical and experimental foundations for further research and development of STING inhibitors as potential drugs for treating ovarian dysfunction.

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Cyst fluid iron-related compounds as useful markers to distinguish malignant transformation from benign endometriotic cysts

Abstract: We conclude for the first time that iron-related compounds are important biomarkers that can predict malignant transformation with high sensitivity and specificity for women with endometriosis.

Cited by 39 publications

References 15 publications

Discrimination of malignant transformation from benign endometriosis using a near‑infrared approach

Discrimination of malignant transformation from benign endometriosis using a near‑infrared approach

Comparison of redox parameters in ovarian endometrioma and its malignant transformation

Activation of cGAS/STING Drives Inflammation and Cellular Senescence of Macrophages in Ovarian Endometrioma Induced by Endometriotic Cyst Fluid

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