Cysteine and histidine residues are involved in <i>Escherichia coli</i> Tn<i>21</i> MerE methylmercury transport

Sone, Yuka; Uraguchi, Shimpei; Takanezawa, Yasukazu; Nakamura, Ryosuke; Pan-Hou, Hidemitsu; Kiyono, Masako

doi:10.1002/2211-5463.12341

Cited by 11 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mercuric reductase MerA is the central enzyme in the microbial mercury detoxification system and merA is a suitable biomarker for examining the functional diversity of Hg detoxification ( Boyd and Barkay, 2012 ). The merE gene is a predicted small ORF immediately following merD in many Gram-negative mer operon sequences, and it is proposed to encode a transporter of Hg 2+ or methylmercury ( Sone et al, 2017 ). MerF could transport ionic mercury from the periplasmic protein MerP across the bilayer to MerA and it plays a similar function as that of MerT ( Barkay et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress

Zheng

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

Mercury-mediated toxicity remains one of the greatest barriers against microbial survival, even though bacterial resistance to mercury compounds can occur. However, the genetic and physiological adaptations of bacteria to mercury stress still remains unclear. Here, we show that the marine bacterium Pseudomonas stutzeri 273 is resistant to 50 μM Hg2+ and removes up to 94% Hg2+ from culture. Using gene homologous recombination and complementation, we show that genes encoding Hg2+-transport proteins MerT, MerP, the mercuric reductase MerA and the regulatory protein MerD are essential for bacterial mercuric resistance when challenged with Hg2+. Further, mercury stress inhibits flagellar development, motility, chemotaxis and biofilm formation of P. stutzeri 273, which are verified by transcriptomic and physiological analyses. Surprisingly, we discover that MerF, a previously reported Hg2+-transporter, determines flagellar development, motility and biofilm formation in P. stutzeri 273 by genetic and physiological analyses. Our results strongly indicate that MerF plays an integral role in P. stutzeri 273 to develop physiological responses to mercury stress. Notably, MerF homologs are also prevalent in different human pathogens. Using this unique target may provide novel strategies to control these pathogenic bacteria, given the role of MerF in flagella and biofilm formation. In summary, our data provide an original report on MerF in bacterial physiological development and suggest that the mer in marine bacteria has evolved through progressive, sequential recruitment of novel functions over time.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress

Zheng

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Since then, the application of this process has been made easier by the transgenic approach (Saravanan et al, 2022). For example, Hg‐declining bacterial strains of Escherichia coli have been modified by mer operon clone establishment and other recombinant DNA methods for bioremediation (Maqsood et al, 2022; Rafeeq et al, 2023; Sone et al, 2017).…”

Section: Microbial Bioremediationmentioning

confidence: 99%

Bioremediation of mercury contaminated soil and water: A review

Kumar,

Chawla

et al. 2023

Land Degrad Dev

View full text Add to dashboard Cite

Mercury (Hg) pollution of soil and water environments is a major global threat to human health, agri‐food systems and ecosystems and industrial activities mainly coal combustion augmented their content in different environmental media. Bioremediation is a nature‐based solution involving microbial‐ and plant‐based (phytoremediation) technologies that clean‐up Hg contaminated sites. Here, we review Hg‐resistant bacteria and how latest insights in our understanding of the cellular and biochemical mechanisms of the mer operon genes responsible for Hg resistance and transformation have facilitated developments in microbial Hg‐bioremediation. We also review the phytoremediation mechanisms, including those of bacterial‐ and fungi‐assisted phytoremediation processes, which have shown promising results in reducing Hg2+ to Hg0. This review provides a detailed knowledge of novel Hg bioremediation mechanisms and methods. Consequently, microbial‐ and phyto‐based bioremediation technologies have a critical role in the reclamation of Hg‐contaminated environments and the protection of human health and ecosystems.

show abstract

“…In addition to MerT, other mercury transporters identified in mercury-resistant bacteria include MerC, MerE, and MerF. We have previously shown that MerE is a mercury transporter and transports Hg 2+ and MeHg, [38][39][40] and MerT transports phenylmercury. 32,41) However, the functions of these transporters remained unclear.…”

Section: Molecular Mechanisms Of Mercurial Resistance In Pseudomonas ...mentioning

confidence: 99%

Molecular Toxicology of Methylmercury and Phytoremediation of Toxic Metals for Human Health

et al. 2023

Self Cite

View full text Add to dashboard Cite

The history of methylmercury (MeHg) toxicity research goes back to Minamata disease. In Japan, histopathological examination of patients afflicted with Minamata disease, alongside meticulous investigations at both in vitro and in vivo, were robustly undertaken to elucidate the deleterious effects of MeHg. It is not an overstatement to assert that Japanese investigations on MeHg toxicity have spearheaded global advancements in this field. Nevertheless, more than half a century has passed since the onset of Minamata disease, yet the intricacies of MeHg toxicity remains enigmatic. Moreover, environmental pollution of mercury and toxic metals is a global problem that needs to be solved. Therefore, our research has focused on, "toxicology of MeHg" and "remediation of mercury and other toxic metals". This review discusses the molecular mechanisms underlying roles of autophagy in MeHg responses of mammalian cells, identification of an anti-MeHg natural product, analysis of bacterial mercury-resistant gene, and plant biotechnology using bacterial transporters for phytoremediation.

show abstract

Cysteine and histidine residues are involved in Escherichia coli Tn21 MerE methylmercury transport

Cited by 11 publications

References 27 publications

Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress

Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress

Bioremediation of mercury contaminated soil and water: A review

Molecular Toxicology of Methylmercury and Phytoremediation of Toxic Metals for Human Health

Contact Info

Product

Resources

About