Cysteine reduced the inhibition of CO2 on heterotrophic denitrification: Restoring redox balance, facilitating iron acquisition and carbon metabolism

Zhang, Xuemeng; Yu, Tong; Liu, Chao; Fan, Xinyun; Wu, Yang; Wang, Meng; Zhao, Chunxia; Chen, Yinguang

doi:10.1016/j.scitotenv.2022.154173

Cited by 13 publications

(4 citation statements)

References 60 publications

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“…The bacterial cell density is a crucial factor for denitrification because the reduction of nitrogen oxides is driven by individual denitrifying microorganisms . As shown in Figure a, BC@β-CD shortened the lag phase of P. denitrificans from 12 to 8 h. P. denitrificans assimilated ammonia nitrogen to support their growth, therefore, the variation of ammonia nitrogen concentration in the medium is positively related to the proliferation of P. denitrificans (Figure b). BC and BC@β-CD elevated the growth rate of P. denitrificans in the logarithmic period (12 and 20 h); more ammonia nitrogen was used for cell reproduction.…”

Section: Resultsmentioning

confidence: 90%

How β-Cyclodextrin-Functionalized Biochar Enhanced Biodenitrification in Low C/N Conditions via Regulating Substrate Metabolism and Electron Utilization

Shi

Feng

Xiao

et al. 2023

Environ. Sci. Technol.

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Biodenitrification plays a vital role in the remediation of nitrogen-contaminated water. However, influent with a low C/N ratio limits the efficiency of denitrification and causes the accumulation/emission of noxious intermediates. In this study, β-cyclodextrin-functionalized biochar (BC@β-CD) was synthesized and applied to promote the denitrification performance of Paracoccus denitrificans when the C/N was only 4, accompanied by increased nitrate reduction efficiency and lower nitrite accumulation and nitrous oxide emission. Transcriptomic and enzymatic activity analyses showed BC@β-CD enhanced glucose degradation by promoting the activities of glycolysis (EMP), the pentose phosphate pathway (PPP), and the tricarboxylic acid (TCA) cycle. Notably, BC@β-CD drove a great generation of electron donors by stimulating the TCA cycle, causing a greater supply of substrate metabolism to denitrification. Meanwhile, the promotional effect of BC@β-CD on oxidative phosphorylation accelerates electron transfer and ATP synthesis. Moreover, the presence of BC@β-CD increased the intracellular iron level, causing further improved electron utilization in denitrification. BC@β-CD helped to remove metabolites and induced positive feedback on the metabolism of P. denitrificans. Collectively, these effects elevated the glucose utilization for supporting denitrification from 36.37% to 51.19%. This study reveals the great potential of BC@β-CD for enhancing denitrification under low C/N conditions and illustrates a potential application approach for β-CD in wastewater bioremediation.

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Section: Resultsmentioning

confidence: 90%

How β-Cyclodextrin-Functionalized Biochar Enhanced Biodenitrification in Low C/N Conditions via Regulating Substrate Metabolism and Electron Utilization

Shi

Feng

Xiao

et al. 2023

Environ. Sci. Technol.

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“…To demonstrate that cysteine could protect bacteria from Cd 2+ entering the cell under cadmium stress and promote the elimination of intracellular ROS and energy production, 23,24 H. neapolitanus was incubated with different Cd 2+ concentrations at 120 h without cysteine as shown in Fig. S9.…”

Section: Resultsmentioning

confidence: 99%

Semiconductor biohybrids for enhanced bifunctional wastewater sulfur and heavy metal removal

Zhang,

Fang,

Qian

et al. 2024

Green Chem.

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“…The measurements of propionate, glucose, NO 3 – -N, NO 2 – -N, N 2 O, and NH 4 + -N were conducted according to the references. , The assays of NADH and NADH/NAD + , EPS, electron transport system activity (ETSA), complex I, complex III, and denitrification enzymes are detailed in the Supporting Information, and the indicators of activity tests were normalized by dividing by OD 600 (biomass concentration).…”

Section: Methodsmentioning

confidence: 99%

Propionibacterium freudenreichii-Assisted Approach Reduces N₂O Emission and Improves Denitrification via Promoting Substrate Uptake and Metabolism

Zhang

et al. 2022

Environ. Sci. Technol.

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N 2 O emission is often encountered during biodenitrification. In this paper, a new approach of using microorganisms to promote substrate uptake and metabolism to reduce denitrification intermediate accumulation was reported. With the introduction of Propionibacterium freudenreichii to a biodenitrification system, N 2 O and nitrite accumulation was, respectively, decreased by 74 and 60% and the denitrification efficiency was increased by 150% at the time of 24 h with P. freudenreichii/ groundwater denitrifier of 1/5 (OD 600 ). Propionate, produced by P. freudenreichii, only accelerated nitrate removal and was not the main reason for the decreased intermediate accumulation. The proteomic and enzyme analyses revealed that P. freudenreichii stimulated biofilm formation by upregulating proteins involved in porin forming, putrescine biosynthesis, spermidine/putrescine transport, and quorum sensing and upregulated transport proteins, which facilitated the uptake of the carbon source, nitrate, and Fe and Mo (the required catalytic sites of denitrification enzymes). Further investigation revealed that P. freudenreichii activated the methylmalonyl-CoA pathway in the denitrifier and promoted it to synthesize heme/heme d1, the groups of denitrification enzymes and electron transfer proteins, which upregulated the expression of denitrifying enzyme proteins and enhanced the ratio of NosZ to NorB, resulting in the increase of generation, transfer, and consumption of electrons in biodenitrification. Therefore, a significant reduction in the denitrification intermediate accumulation and an improvement in the denitrification efficiency were observed.

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Cysteine reduced the inhibition of CO2 on heterotrophic denitrification: Restoring redox balance, facilitating iron acquisition and carbon metabolism

Cited by 13 publications

References 60 publications

How β-Cyclodextrin-Functionalized Biochar Enhanced Biodenitrification in Low C/N Conditions via Regulating Substrate Metabolism and Electron Utilization

How β-Cyclodextrin-Functionalized Biochar Enhanced Biodenitrification in Low C/N Conditions via Regulating Substrate Metabolism and Electron Utilization

Semiconductor biohybrids for enhanced bifunctional wastewater sulfur and heavy metal removal

Propionibacterium freudenreichii-Assisted Approach Reduces N₂O Emission and Improves Denitrification via Promoting Substrate Uptake and Metabolism

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Cysteine reduced the inhibition of CO2 on heterotrophic denitrification: Restoring redox balance, facilitating iron acquisition and carbon metabolism

Cited by 13 publications

References 60 publications

How β-Cyclodextrin-Functionalized Biochar Enhanced Biodenitrification in Low C/N Conditions via Regulating Substrate Metabolism and Electron Utilization

How β-Cyclodextrin-Functionalized Biochar Enhanced Biodenitrification in Low C/N Conditions via Regulating Substrate Metabolism and Electron Utilization

Semiconductor biohybrids for enhanced bifunctional wastewater sulfur and heavy metal removal

Propionibacterium freudenreichii-Assisted Approach Reduces N2O Emission and Improves Denitrification via Promoting Substrate Uptake and Metabolism

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Product

Resources

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Propionibacterium freudenreichii-Assisted Approach Reduces N₂O Emission and Improves Denitrification via Promoting Substrate Uptake and Metabolism