Cystic craniopharyngioma: trans-sphenoidal surgery and intra-cystic apposition of “bleomycin wax”

Fraioli, Mario Francesco; Moschettoni, Laura; Catena, Elisabetta; Fraioli, Chiara

doi:10.1007/s00701-009-0320-7

Cited by 5 publications

(1 citation statement)

References 19 publications

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“…Adamantinomatous craniopharyngioma (ACP) often presents as large or huge mass, with invasion of neighboring eloquent or vital neurovascular structures, and high recurrence rate, even following gross total surgical resection. Because approximately 90% of ACP include a cystic component, multiple efforts have been directed toward assessing the effectiveness of intracystic therapies, such as intratumoral bleomycin , or to develop new ones, such as intracystic interferon‐α . Similarly, a new avenue in basic research on ACP is the characterization of cyst fluid (previous work summarized in Table ), including the application of proteomic analysis (PA).…”

Section: Introductionmentioning

confidence: 99%

Proteomics in pediatric cystic craniopharyngioma

et al. 2017

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Adamantinomatous craniopharyngioma (ACP) is still often burdened by a poor prognosis in children as far as the risk of recurrence and the quality of life are concerned. Therefore, many efforts are now dedicated to investigate the molecular characteristics of this tumor aiming at finding new therapeutic options. ACP is prevalently a cystic lesion so that an increasing number of researches are focused on the analysis of its cystic content. In the present article, the main results of the current proteomic analysis (PA) on the ACP fluid are summarized. Both "bottom-up" and "top-down" approaches have been utilized. In the bottom-up approach, proteins and peptides are enzymatically or chemically digested prior to liquid chromatography and mass spectrometry analyses. The bottom-up approach pointed out several proteins of the inflammation (namely, α2-HS-glycoprotein, α1-antichymotrypsin and apolipoproteins) as possibly involved in the genesis and growth of the cystic component of ACP. The top-down strategy analyzes proteins and peptides in the intact state, making it particularly suitable for the identification of peptides and low molecular weight proteins and for the characterization of their possible isoforms and post-translational modifications. The top-down approach disclosed the presence of the thymosin β family. Thymosin β4, in particular, which is involved in the cytoskeleton organization and migration of several tumors, could play a role in the progression of ACP. Finally, PA was utilized to investigate alterations in cyst fluid character after treatment with interferon-α. The analyzed samples showed a progressive reduction of the levels of α-defensins (proteins involved in the inflammatory-mediated response) after the intracystic injection of interferon-α, thus reinforcing the hypothesis that inflammation contributes to ACP cyst pathogenesis. Additional studies on the solid component of ACP are still necessary to further validate the previous results and to identify possible markers for targeted therapy.

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Section: Introductionmentioning

confidence: 99%