Cystine Aminopeptidase Activity in Pregnancy

Rydén, Gunnar

doi:10.3109/00016347409157749

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…Even with careful urine collection the individual day-to-day Variation is rather pronounced (the coefficient of variations was 16% in the present study). Many reports also indicate that estriol assays not always reflect the condition of the feto-placental unit [6,15,19], and much research has during the last decade been directed towards new methods for evaluating the fetoplacental unit. In recent years much attention has been directed towards the determination of HCS äs a possible mean of monitoring the placental function [1,11,13,20,21].…”

Section: Discussionmentioning

confidence: 99%

“…CURZEN and VARMA [7] who compared CAP and urinary estriol assays concluded that CAP was of no value in predicting the condition of the feto-placental unit. Other authors [3,4,9,17,19,22] Comparative studies using all three parameters are scanty. KRISTENSEN et al [5] compared HCS, CAP and urinary estriol excretion in pregnancies complicated with essential hypertension and preeclampsia.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

CAP, HCS and urinary estriol measurements in risk pregnancies – A comparative study

Rydén¹,

Kågedal²

1977

Journal of Perinatal Medicine

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

CAP, HCS and urinary estriol measurements in risk pregnancies – A comparative study

Rydén¹,

Kågedal²

1977

Journal of Perinatal Medicine

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Radioimmunoassay of vasopressin during pregnancy. Use and removal of cystylaminopeptidase inhibitors

Post¹,

Heerikhuize²,

Boer³

et al. 1994

Clinica Chimica Acta

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This paper describes a radioimmunoassay (RIA) for arginine-vasopressin in which o-phenanthroline effectively inhibits cystyl-amino-peptidase activity in whole blood and plasma from pregnant women but in which o-phenanthroline is removed during the extraction of vasopressin from plasma to prevent disturbance of the RIA. Cystyl-amino-peptidase causes immediate degradation of vasopressin unless cystyl-amino-peptidase enzyme inhibitors such as o-phenanthroline are applied. However, o-phenanthroline interferes with RIA. We report an extraction procedure over octadecasyl silica-packed Sep-Pak C18 columns, by which cystyl-amino-peptidase as well as most of the cystyl-amino-peptidase inhibitor is removed from plasma with chloroform. The average o-phenanthroline concentration (0.25 mmol/l) found in the assay medium after extraction appeared not to interfere with the RIA. Polyacrylamide gel isoelectric focusing of extracts of platelet-rich and platelet-poor plasma from pregnant women revealed a single vasopressin immunoreactive peak in the RIA. Recovery and between-assay coefficients of variation of 3.2 ng/l vasopressin from pregnancy whole blood were comparable with non-pregnant controls (57%/8% and 59%/13%, respectively). Results with this assay compare well with those of another assay using inhibitors in pregnant subjects and with results in non-pregnant subjects.

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The value of serum cystine aminopeptidase (cap), human chorionic somato‐mammotrophin (hcs), and urinary oestrogen assays for detecting intrauterine growth retardation

Rydén

1978

Acta Obstet Gynecol Scand

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Abstract. The aim of the present study was to compare the usefulness of cystine aminopeptidase (CAP) and human chorionic somato‐mammotrophin (HCS) estimations in maternal serum and maternal urinary oestrogen excretion in predicting intra‐uterine growth retardation. The material consists of 43 patients who subsequently gave birth to infants with a birth weight less than 2 S.D. from the mean according to the gestational age. The patients were controlled with simultaneous analyses of CAP, HCS and urinary oestrogen assays every week from the 36th week of pregnancy. The patients have been compared with another group in which infants with normal birth weight and without signs of fetal distress were delivered. Intrauterine growth retardation (IGR) was predicted by low oestrogen levels in 58%, low HCS levels in 42% and low CAP levels in 35%. The difference between the biochemical tests was not statistically significant. IGR infants who developed fetal distress were predicted by oestrogen assays in 72%, by HCS assays in 62% and by CAP assays in 48%. If urinary oestrogen assays were combined with HCS assays this combination predicted IGR significantly better than oestrogen assays alone. The combination HCS‐CAP was as informative in this respect as urinary oestrogen assays alone. The results indicate that all methods tested are rather insensitive in predicting the total number of IGR infants. The capacity for predicting IGR infants with fetal distress is, however, rather high. For this purpose a combination of tests is preferable as no test fulfils the qualifications of being superior to the other. The combination oestrogens–HCS seems to be most suitable in this respect.

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Cystine Aminopeptidase Activity in Pregnancy

Cited by 4 publications

References 9 publications

CAP, HCS and urinary estriol measurements in risk pregnancies – A comparative study

CAP, HCS and urinary estriol measurements in risk pregnancies – A comparative study

Radioimmunoassay of vasopressin during pregnancy. Use and removal of cystylaminopeptidase inhibitors

The value of serum cystine aminopeptidase (cap), human chorionic somato‐mammotrophin (hcs), and urinary oestrogen assays for detecting intrauterine growth retardation

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