Cystometric Evaluation of Bladder Function in Non-Anesthetized Mice With and Without Bladder Outlet Obstruction

Pandita, Raj K.; Fujiwara, Masayo; Alm, Per; Andersson, Karl‐Erik

doi:10.1097/00005392-200010000-00079

Cited by 32 publications

(49 citation statements)

References 22 publications

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“…In large parts of the detrusor, the smooth muscle bundles were completely devoid of accompanying VAChT-immunoreactive varicose terminals. In other areas, the densities of nerve structures were nearly normal (Pandita et al, 2000a).…”

Section: Peripheral Targetsmentioning

confidence: 98%

“…Thus, detrusor denervation as a consequence of outflow obstruction has been demonstrated in several species, including humans (Gosling et al, 1986;Sibley, 1987;Speakman et al, 1987;Pandita et al, 2000a). In pigs with experimental outflow obstruction, detrusor response to intramural nerve stimulation was decreased, but there was a supersensitivity to acetylcholine (Sibley, 1984).…”

Section: Peripheral Targetsmentioning

confidence: 99%

See 1 more Smart Citation

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Andersson

Wein²

2004

Pharmacol Rev

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456

364

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Section: Peripheral Targetsmentioning

confidence: 98%

Section: Peripheral Targetsmentioning

confidence: 99%

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Andersson

Wein²

2004

Pharmacol Rev

Self Cite

456

364

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“…Additionally, the protocol above describes use of PE-50 tubing for the rat 13 , however, even larger size catheters, up to PE-100 have been used, especially for long-term studies 14 . In mice, a smaller caliber such as PE-10 tubing can be utilized 15,16 , but it should be kept in mind that smaller, more pliable tubes may not transmit pressure changes to the transducer accurately. Also, the alternative method of securing the catheter on the dorsum (step 8* above) is done in mice due to their smaller body size and the blunt tip needle and IV cap are too cumbersome.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Seth

Gil

et al. 2012

JoVE

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Renal function and continence of urine are critically dependent on the proper function of the urinary bladder, which stores urine at low pressure and expels it with a precisely orchestrated contraction. A number of congenital and acquired urological anomalies including posterior urethral valves, benign prostatic hyperplasia, and neurogenic bladder secondary to spina bifida/spinal cord injury can result in pathologic tissue remodeling leading to impaired compliance and reduced capacity 1 . Functional or anatomical obstruction of the urinary tract is frequently associated with these conditions, and can lead to urinary incontinence and kidney damage from increased storage and voiding pressures 2 . Surgical implantation of gastrointestinal segments to expand organ capacity and reduce intravesical pressures represents the primary surgical treatment option for these disorders when medical management fails 3 . However, this approach is hampered by the limitation of available donor tissue, and is associated with significant complications including chronic urinary tract infection, metabolic perturbation, urinary stone formation, and secondary malignancy 4,5 . Current research in bladder tissue engineering is heavily focused on identifying biomaterial configurations which can support regeneration of tissues at defect sites. Conventional 3-D scaffolds derived from natural and synthetic polymers such as small intestinal submucosa and polyglycolic acid have shown some short-term success in supporting urothelial and smooth muscle regeneration as well as facilitating increased organ storage capacity in both animal models and in the clinic 6,7 . However, deficiencies in scaffold mechanical integrity and biocompatibility often result in deleterious fibrosis 8 , graft contracture 9 , and calcification 10 , thus increasing the risk of implant failure and need for secondary surgical procedures. In addition, restoration of normal voiding characteristics utilizing standard biomaterial constructs for augmentation cystoplasty has yet to be achieved, and therefore research and development of novel matrices which can fulfill this role is needed.In order to successfully develop and evaluate optimal biomaterials for clinical bladder augmentation, efficacy research must first be performed in standardized animal models using detailed surgical methods and functional outcome assessments. We have previously reported the use of a bladder augmentation model in mice to determine the potential of silk fibroin-based scaffolds to mediate tissue regeneration and functional voiding characteristics. 11,12 Cystometric analyses of this model have shown that variations in structural and mechanical implant properties can influence the resulting urodynamic features of the tissue engineered bladders 11,12 . Positive correlations between the degree of matrixmediated tissue regeneration determined histologically and functional compliance and capacity evaluated by cystometry were demonstrated in this model 11,12 . These results therefore suggest that fun...

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“…involuntary detrusor contractions), an inefficient bladder emptying, which leads to increased residual volume, will result in increase in voiding frequency and reduction in voiding volume. It is well established that residual volume is significantly increased in bladder outflow obstruction in human patients and animal models (Rosier et al, 1995;O'Connor et al, 1997;Robertson, 1999;Pandita et al, 2000). The mechanism responsible for increased residual volume and bladder capacity in obstruction is still unclear.…”

Section: Other Factors Influenced Obstruction-induced Bladder Overactmentioning

confidence: 99%

Conscious voiding during bladder obstruction in guinea pigs correlates with contractile activity of isolated bladders

Nicholas

Keightley

Brookes

et al. 2015

Autonomic Neuroscience

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Purpose: There are many hypotheses accounting for detrusor overactivity, however the exact mechanisms are still incompletely understood. We used a model of bladder outlet obstruction in male guinea pigs as a way to produce detrusor overactivity. The objective was to determine whether changes in voiding of obstructed guinea pigs correlates with specific changes in contractile activity of their isolated bladders in vitro. Material and methods: Conscious voiding activity of sham-operated and obstructed animals was measured in metabolic cages. Contractile activity (spontaneous or evoked by distension, electrical field stimulation or cholinergic agonists) was recorded via a pressure transducer in the isolated bladders in vitro. Results: The frequency of conscious voiding increased (while voiding volume decreased) in the obstructed group, compared with the sham-operated group, 4 weeks after surgical intervention. In comparison to the sham-operated animals, the bladders from the obstructed guinea pigs were enlarged and inflamed, their frequency of spontaneous contractions was higher, while the amplitudes of electrical field stimulation (EFS)-induced contractions and bladder compliance were lower. Changes in conscious voiding during obstruction were significantly associated with alterations in structural parameters (bladder weight, thickness and histological damage score) and functional contractile parameters (frequency of spontaneous contractions, amplitude of EFS-induced contractions and bladder compliance) of their isolated bladders. Conclusions: Our findings revealed significant association between conscious voiding and structural and contractile activity changes of the isolated bladders in obstruction. The data suggest that change in contractile activity of the bladder itself is a major contributor to obstruction-induced bladder overactivity.

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Cystometric Evaluation of Bladder Function in Non-Anesthetized Mice With and Without Bladder Outlet Obstruction

Cited by 32 publications

References 22 publications

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Pharmacology of the Lower Urinary Tract: Basis for Current and Future Treatments of Urinary Incontinence

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Conscious voiding during bladder obstruction in guinea pigs correlates with contractile activity of isolated bladders

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