Cyt‐Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2019 Conference Workshops

Czechowska, Kamila; Lannigan, Joanne; Aghaeepour, Nima; Back, Jessica B.; Begum, Julfa; Behbehani, Greg K.; Bispo, Cláudia; Bitoun, Daniel; Fernández, Alfonso Blanco; Boova, Samuel Tony; Brinkman, Ryan R.; Ciccolella, Christopher O.; Cotleur, Bunny; Davies, Derek; Cruz, Gelo Victoriano Dela; Rio-Guerra, Roxana del; Lauriers‐Cox, Ann Marie Des; Douagi, Iyadh; Dumrese, Claudia; Escobar, Diana L. Bonilla; Estevam, Jose; Ewald, Christina; Fossum, Anna; Gaudillière, Brice; Green, Cherie; Groves, Christopher J.; Hall, Christopher; Haque, Yasmin; Hedrick, Michael N.; Hogg, Karen; Hsieh, Elena W.Y.; Irish, Jonathan M.; Lederer, James A.; Maecker, Holden; Lewis‐Tuffin, Laura J.; Litwin, Virginia; Lopez, Peter; Nasdala, Ines; Nedbal, Jakub; Ohlsson‐Wilhelm, Betsy M.; Price, Kylie M.; Rahman, Adeeb; Rayanki, Radhika; Rieger, Aja M.; Robinson, J. Paul; Shapiro, Howard; Sun, Yongliang; Tang, Vera A.; Tesfa, Lydia; Telford, William G.; Walker, Rachael; Welsh, Joshua; Wheeler, Paul S.; Tárnok, Attila

doi:10.1002/cyto.a.23941

Cited by 10 publications

(7 citation statements)

References 46 publications

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“…Instrument vendors have also made great advances in user‐friendliness of instrument operation and QC, facilitating end‐user independence (3). Establishing a system within SRLs to empower end‐users to assist in these roles will be advantageous as more SRLs strive to provide expanded services and throughput without a commensurate expansion of staffing (4–6). However, SRL managers must also set clear limits on which procedures should be attempted by users and which should require staff consultation.…”

Section: Resources For End‐user Self‐service and Troubleshootingmentioning

confidence: 99%

Solutions for Shared Resource Lab Remote Quality Control and Instrument Troubleshooting during a Pandemic

et al. 2020

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The COVID‐19 pandemic has dramatically affected shared resource lab (SRL) staff in‐person availability at institutions globally. This article discusses the challenges of ensuring reliable instrument performance and quality data output while facility staff and external service provider on‐site presence is severely limited. Solutions revolve around the adoption of remote monitoring and troubleshooting platforms, provision of self‐service troubleshooting resources specific to facility instruments and workflows, development of an assistance contact policy, and ensuring efficiency of limited in‐person staff time. These solutions employ software and hardware tools that are already in use or readily available in the SRL community, such as remote instrument access tools, video hosting and conferencing platforms, and ISAC shared resources. © 2020 International Society for Advancement of Cytometry

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Section: Resources For End‐user Self‐service and Troubleshootingmentioning

confidence: 99%

Solutions for Shared Resource Lab Remote Quality Control and Instrument Troubleshooting during a Pandemic

et al. 2020

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“…There is currently no consensus method for this. This study was carried out for a CYTO2019 Workshop where the feasibility of combining scatter and fluorescence calibration for small particle FCM was presented (13). Calibrations were performed using FCM PASS software package with commercially available reference materials to convert fluorescence intensity to MESF and light scatter to diameter.…”

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confidence: 99%

Fluorescence and Light Scatter Calibration Allow Comparisons of Small Particle Data in Standard Units across Different Flow Cytometry Platforms and Detector Settings

2020

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Flow cytometers have been utilized for the analysis of submicron‐sized particles since the late 1970s. Initially, virus analyses preceded extracellular vesicle (EV), which began in the 1990s. Despite decades of documented use, the lack of standardization in data reporting has resulted in a growing body of literature that cannot be easily interpreted, validated, or reproduced. This has made it difficult for objective assessments of both assays and instruments, in‐turn leading to significant hindrances in scientific progress, specifically in the study of EVs, where the phenotypic analysis of these submicron‐sized vesicles is becoming common‐place in every biomedical field. Methods for fluorescence and light scatter standardization are well established and the reagents to perform these analyses are commercially available. However, fluorescence and light scatter calibration are not widely adopted by the small particle community as methods to standardize flow cytometry (FCM) data. In this proof‐of‐concept study carried out as a resource for use at the CYTO2019 workshop, we demonstrate for the first‐time simultaneous fluorescence and light scatter calibration of small particle data to show the ease and feasibility of this method for standardized FCM data reporting. This data was acquired using standard configuration commercial flow cytometers, with commercially available materials, published methods, and freely available software tools. We show that application of light scatter, fluorescence, and concentration calibration can result in highly concordant data between FCM platforms independent of instrument collection angle, gain/voltage settings, and flow rate; thus, providing a means of cross comparison in standard units. © 2020 International Society for Advancement of Cytometry

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“…As the mass cytometry community grew larger and the technology gained maturity through multiple iterative assay and hardware improvements, the focus of the Forum shifted from technical aspects toward customized workflows to address diverse applications in immunology, immune‐oncology, oncology, biology, nanotoxicology, biomarker identification, pathogenesis of immune‐mediated diseases such as rheumatoid arthritis, SLE and multiple sclerosis, spanning works with human, mouse and drosophila cells. This development parallels the results from mass cytometry workshops held at CYTO . Many projects presented at the Forum were later published in peer‐reviewed journals .…”

Section: The German Mass Cytometry User Forum In Figuresmentioning

confidence: 56%

“…). Speakers included Michael Leipold from Stanford University, giving this year`s ISAC lecture addressing the challenges of large mass cytometry studies that have been identified as a major hurdle for the implementation of mass cytometry to monitor clinical trials . There, unwanted data variation can be minimized by, for example, barcoding, and the careful decision of which samples to combine into a barcoded pool, implementation of instrument and assay controls, and antibody cocktail preservation to minimize reagent variation.…”

Section: The German Mass Cytometry User Forum In Figuresmentioning

confidence: 99%

Mass Cytometry—A Tool for the Curious: Networking in Berlin

et al. 2020

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Cyt‐Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2019 Conference Workshops

Cited by 10 publications

References 46 publications

Solutions for Shared Resource Lab Remote Quality Control and Instrument Troubleshooting during a Pandemic

Solutions for Shared Resource Lab Remote Quality Control and Instrument Troubleshooting during a Pandemic

Fluorescence and Light Scatter Calibration Allow Comparisons of Small Particle Data in Standard Units across Different Flow Cytometry Platforms and Detector Settings

Mass Cytometry—A Tool for the Curious: Networking in Berlin

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