Cytidine-to-Uridine RNA Editing Factor NbMORF8 Negatively Regulates Plant Immunity to <i>Phytophthora</i> Pathogens

Yang, Yang; Fan, Guangjin; Zhao, Yan; Wen, Qujiang; Wu, Peng; Meng, Yuling; Shan, Weixing

doi:10.1104/pp.20.00458

Cited by 24 publications

(21 citation statements)

References 71 publications

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“…A previous study determined that one gene, overexpressor of cationic peroxidase3 (ocp3), contributed to control of the extent of ndhB transcripts' editing, and proposed that in ocp3-mediated chloroplast RNA editing in plant immunity, ocp3 mutants lead to ndhB editing efficiency decays, thereby impairing cyclic electron flow (CEF) and substantially enhancing disease resistance to fungal pathogens [32]. Recent evidence indicated that MORF genes were vital for plant development and stress response, such as drought stress in poplar, seedling survival in rice, and pathogen stress in tobacco [7,17,19]. Another study also found that NbMORF8 localized in the mitochondrion negatively regulates plant immunity to Phytophthora pathogens [19], indicating the roles of nuclear gene regulation in plant enhanced resistance.…”

Section: Discussionmentioning

confidence: 99%

“…Plants 2021, 10, x 14 of 18 resistance to fungal pathogens [32]. Recent evidence indicated that MORF genes were vital for plant development and stress response, such as drought stress in poplar, seedling survival in rice, and pathogen stress in tobacco [7,17,19]. Another study also found that NbMORF8 localized in the mitochondrion negatively regulates plant immunity to Phytophthora pathogens [19], indicating the roles of nuclear gene regulation in plant enhanced resistance.…”

Section: Discussionmentioning

confidence: 99%

“…MORFs are a small protein family in land plants. Members of the MORF gene family have been widely identified in genomes of several species, such as A. thaliana with nine members, P. trichocarpa with nine [7], O. sativa with seven [17], Z. mays with seven [18], and Nicotiana with nine [19]. MORF proteins harbor a conserved stretch of residues (MORFbox), and form homo-and heteromers to interact with selected PPR proteins; however, the molecular function of the MORF-box remains elusive because it shares no sequence similarity with known domains [20].…”

Section: Introductionmentioning

confidence: 99%

“…In poplar, it has been reported that the PtrMORF genes responded to drought [7]. In recent studies, mitochondrion-localized NbMORF8 in tobacco was reported to negatively regulate plant immunity to pathogens [19]. OsMORF9 plays a critical role in the biogenesis of chloroplast ribosomes, chloroplast development, and seedling survival [24].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Analysis of Multiple Organellar RNA Editing Factor (MORF) Family in Kiwifruit (Actinidia chinensis) Reveals Its Roles in Chloroplast RNA Editing and Pathogens Stress

Xiong

Fang

Jiang

et al. 2022

Plants

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Kiwifruit (Actinidia chinensis) is well known for its high vitamin C content and good taste. Various diseases, especially bacterial canker, are a serious threat to the yield of kiwifruit. Multiple organellar RNA editing factor (MORF) genes are pivotal factors in the RNA editosome that mediates Cytosine-to-Uracil RNA editing, and they are also indispensable for the regulation of chloroplast development, plant growth, and response to stresses. Although the kiwifruit genome has been released, little is known about MORF genes in kiwifruit at the genome-wide level, especially those involved in the response to pathogens stress. In this study, we identified ten MORF genes in the kiwifruit genome. The genomic structures and chromosomal locations analysis indicated that all the MORF genes consisted of three conserved motifs, and they were distributed widely across the seven linkage groups and one contig of the kiwifruit genome. Based on the structural features of MORF proteins and the topology of the phylogenetic tree, the kiwifruit MORF gene family members were classified into six groups (Groups A–F). A synteny analysis indicated that two pairs of MORF genes were tandemly duplicated and five pairs of MORF genes were segmentally duplicated. Moreover, based on analysis of RNA-seq data from five tissues of kiwifruit, we found that both expressions of MORF genes and chloroplast RNA editing exhibited tissue-specific patterns. MORF2 and MORF9 were highly expressed in leaf and shoot, and may be responsible for chloroplast RNA editing, especially the ndhB genes. We also observed different MORF expression and chloroplast RNA editing profiles between resistant and susceptible kiwifruits after pathogen infection, indicating the roles of MORF genes in stress response by modulating the editing extend of mRNA. These results provide a solid foundation for further analyses of the functions and molecular evolution of MORF genes, in particular, for clarifying the resistance mechanisms in kiwifruits and breeding new cultivars with high resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genome-Wide Analysis of Multiple Organellar RNA Editing Factor (MORF) Family in Kiwifruit (Actinidia chinensis) Reveals Its Roles in Chloroplast RNA Editing and Pathogens Stress

Xiong

Fang

Jiang

et al. 2022

Plants

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“…For western blot analyses, SDS-PAGE, electroblotting, and immunodetection analysis were carried out as described previously (Yang et al, 2020). Precipitated proteins were detected with an anti-GFP antibody (#A02020; Abbkine), an anti-mCherry antibody (#A02080; Abbkine), and a goat anti-mouse secondary antibody (ab6789; Abcam).…”

Section: Co-ip and Western Blot Analysismentioning

confidence: 99%

Proteomics and Functional Analysis Revealed TaGSTU6/TaCBSX3 Enhances Wheat Resistance to Powdery Mildew

Wang

Guo

Jin

et al. 2022

Preprint

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Wheat stripe rust and powdery mildew are important worldwide diseases of wheat (Triticum aestivum). The wheat cultivar Xingmin318 (XM318) is resistant to both wheat stripe rust and powdery mildew, which are caused by Puccinia striiformis f. sp. tritici (Pst) and Blumeria graminis f. sp. tritici (Bgt), respectively. To explore the molecular mechanisms of wheat defenses against Pst and Bgt, quantitative proteomic analyses of XM318 inoculated with Pst and Bgt, respectively, were performed using tandem mass tags (TMT) technology. A total of 741 proteins were identified as differentially accumulated proteins (DAPs). Bioinformatics analyses indicated that some functional categories, including antioxidant activity, exhibited obvious differences between Pst and Bgt infections. Intriguingly, only 42 DAPs responded to both Pst and Bgt infections. Twelve DAPs were randomly selected for RT-qPCR analysis, and the mRNA expression levels of eleven were consistent with their protein expression. Furthermore, gene silencing using the virus-induced gene silencing system indicated that glutathione S-transferase (TaGSTU6) has an important role in resistance to Bgt but not to Pst. TaGSTU6 was shown to interact with the cystathionine beta-synthase (CBS) domain-containing protein (TaCBSX3). Knockdown of TaCBSX3 expression only reduced wheat resistance to Bgt infection. Overexpression of TaGSTU6 and TaCBSX3 in Arabidopsis (Arabidopsis thaliana) promoted plant resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Our results indicated that the TaGSTU6 interacting with TaCBSX3 only confers wheat resistance to Bgt, suggesting that wheat has different response mechanisms to Pst and Bgt stress.One-sentence summaryProteomics revealed a difference in the wheat resistance response to Pst and Bgt, and the TaGSTU6/TaCBSX3 interaction plays an important role only in wheat resistance to Bgt.

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RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts

Ali,

Song,

Zhang

et al. 2024

Advanced Science

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RPOTp is the nuclear‐encoded plastid‐targeted RNA polymerase and plays a crucial role in chloroplast gene expression. Transcripts in plant organelles are altered by the conversion of cytidine (C) to uridine (U) at specific positions through RNA editing. However, whether RPOTp is involved in chloroplast RNA editing remains unclear. Here, the role of RPOTp in C‐to‐U RNA editing at multiple sites in Arabidopsis chloroplasts is uncovered. Multiple organellar RNA editing factor 2 (MORF2) is required for the editing of most sites in chloroplasts. RPOTp is identified from the co‐immunoprecipitation targets of MORF2. The sca3‐2 mutant, defective in RPOTp, exhibits a pale‐yellow phenotype and alters the RNA editing of nine sites in chloroplasts. It is also shown that RNA editing is uncoupled from chloroplast transcriptional activity. RPOTp directly interacts with chloroplast multiple‐site RNA editing factors, including MORF2, MORF8, MORF9, and ORRM1. It is further shown that RPOTp participates in RNA editing by influencing the dimerization of MORF proteins. The defect in RPOTp impairs the expression of most chloroplast genes, indicating an indispensable role for RPOTp in chloroplast gene expression. These findings reveal that RPOTp not only participates in transcription but also has a novel role in RNA editing of chloroplast transcripts.

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Cytidine-to-Uridine RNA Editing Factor NbMORF8 Negatively Regulates Plant Immunity to Phytophthora Pathogens

Cited by 24 publications

References 71 publications

Genome-Wide Analysis of Multiple Organellar RNA Editing Factor (MORF) Family in Kiwifruit (Actinidia chinensis) Reveals Its Roles in Chloroplast RNA Editing and Pathogens Stress

Genome-Wide Analysis of Multiple Organellar RNA Editing Factor (MORF) Family in Kiwifruit (Actinidia chinensis) Reveals Its Roles in Chloroplast RNA Editing and Pathogens Stress

Proteomics and Functional Analysis Revealed TaGSTU6/TaCBSX3 Enhances Wheat Resistance to Powdery Mildew

RNA Polymerase RPOTp is Involved in C‐to‐U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts

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