Cyto-genotoxic effects of Pinoxaden on Allium cepa L. roots

Aydin, Gamze; Liman, Recep

doi:10.1007/s13353-020-00560-w

Cited by 12 publications

(6 citation statements)

References 37 publications

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“…Genotoxicity of the used herbicide is higher than normal with use of the highest concentration (1.6 ppm) for each application period. These outcomes agree with former studies (Ping et al, 2012;Karaismailoğlu et al, 2013;Karaismailoğlu, 2014bKaraismailoğlu, , 2015Bonciu et al, 2018;Liman and Özkan, 2019;Rosculete et al,2019;Aydın and Liman, 2020).…”

Section: Discussionsupporting

confidence: 93%

Allium cepa'nın Somatik Hücreleri Üzerine Oxyfluorfen’in Sitotoksik ve Genotoksik Etkileri

Karaismailoğlu

2022

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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In this work, the cytotoxic and genotoxic influences of oxyfluorfen herbicide were surveyed by examining of mitotic index, mitotic stages, chromosomal anomalies, micronucleus percentage, and comet assay parameters on the somatic cells of Allium cepa. The roots were treated with 0.2, 0.4, 0.8 and 1.6 ppm herbicide concentrations with 12, 24 and 36 h. application periods. Mitotic index was noticeably diminished by oxyfluorfen in each application group when matched with their control, except for 0.2 ppm. The percentages of mitotic stages were altered. Oxyfluorfen enhanced drastically the aberrant cell ratio at all application groups and application periods in contrast to their control, excluding 0.2 ppm. Mitotic anomalies were noted as disturbed prophase, stickiness, C-mitosis, chromatid bridges and laggards. The micronucleus was detected at interphase and its percentage was determined in the applied concentrations. Also, the comet assay was employed to examine the single strand breakages. Almost all of the used concentrations of oxyfluorfen increased DNA losses. A positive relationship was discovered between micronucleus occurrence and DNA loss.

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Section: Discussionsupporting

confidence: 93%

Allium cepa'nın Somatik Hücreleri Üzerine Oxyfluorfen’in Sitotoksik ve Genotoksik Etkileri

Karaismailoğlu

2022

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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“…Pinoxaden showed cytotoxic effects by decreasing the root growth and MI. Pinoxaden induced CAs including anaphase bridges, chromosome laggards, stickiness and polyploidy (Aydın and Liman, 2020) In our research genotoxic effects of spirotetramat on A. cepa root tips have been determined on the basis of chromosomal aberrations and mitotic index. The aberrations were observed in each concentration of spirotetramat application.…”

Section: Discussionmentioning

confidence: 97%

“…In another research, cyto-genotoxic effects of pinoxaden on the Allium cepa roots were investigated. Mitotic index (MI), mitotic phases, chromosomal aberrations (Aydın and Liman, 2020) The study and study of lettuce-cultivated agricultural areas in the Mediterranean region found more than necessary pesticides were used in the fight against leaf aphids than necessary, and in the research conducted for the solution, Nasonovia ribisnigri, Rhopalosiphum nymhaeae, Aulacorthum solani were battled with 7 different leaf aphids, particularly. Spirotetramat and Thiomethoxam active ingredients used in the control ensured that the populations of harmful organisms were kept below the economic damage in the sprayings made before the core of the lettuce was formed (Sangün, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Effects of Spirotetramat on Allium Cepa L.

2021

JSTR

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In this research the genotoxic effects of spirotetramat have been determined by the meaning of mitotic index (MI) and chromosomal abnormality in Allium cepa L. root tip meristematic cells. For this purpose the Allium test was carried out exposing roots to spirotetramat for 24 and 48 hours at the concentrations of 1 mL/L, 2 mL/L, 4 mL/L. After the spirotetramat applications, root tips have been harvested and standard mitotic test procedures have been realized. The mitotic index was calculated as the number of dividing cells per number of 1000-1500 observed cells and the chromosomal aberrations also were scored for each group. Our results have showed that the mitotic index significantly decreased with the increase of doses and exposure times when compare to their controls. According to our results, MI value has been decreased as 56% and 65% in 4 mL/L spirotetramat application after 24 and 48 hours respectively. As a result of our research, it was understood that the chromosomal aberration frequency increased in all application groups when we compare with the control group. According to chromosomal aberration frequency results, when we compare with control group chromosome aberration frequency increased approximately seven fold in 4 mL/L spirotetramat applications after 24 and 48 hours respectively. Our results showed that spirotetramat induced significant increases of chromosomal aberrations such as table and pole shifting, chromosomal bridge. In addition that mitotic index (MI) decreased significantly with increasing of spirotetramat doses and the exposure time as compared to their controls.

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“…When the assay is to be performed with leaves, calibration tests must use a mutagen [16]. The most frequently applied chemical DNA strand break inducers in plants are the alkylating agents ethyl methanesulfonate (EMS), methyl metanosulphonate (MMS), and Methylnitrosourea (MNU), and the radiomimetics bleomycin and zeocin [4,102,[109][110][111][112][113][114][115][116][117][118][119][120][121][122][123][124][125][126][127]. MMS, EMS, and MNU are capable of inducing a variety of lesions including adducts, cross-links, and breaks in the DNA chain; they can modify DNA, adding alkyl groups in several positions of the DNA bases.…”

Section: Calibration and Positive Controlsmentioning

confidence: 99%

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Tyutereva,

Strizhenok,

Kiseleva

et al. 2024

Horticulturae

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Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications; however, they are still relatively rarely used in physiological studies. In this review, we provide an overview of the basic principles and of useful variations of the protocols of Comet assays, as well as of their use in plant studies, in order to encourage plant physiologists to include this tool in the analysis of plant stress responses.

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Cyto-genotoxic effects of Pinoxaden on Allium cepa L. roots

Cited by 12 publications

References 37 publications

Allium cepa'nın Somatik Hücreleri Üzerine Oxyfluorfen’in Sitotoksik ve Genotoksik Etkileri

Allium cepa'nın Somatik Hücreleri Üzerine Oxyfluorfen’in Sitotoksik ve Genotoksik Etkileri

Genotoxic Effects of Spirotetramat on Allium Cepa L.

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

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