Cyto-genotoxicity and oxidative stress induced by zinc oxide nanoparticle in human lymphocyte cells in vitro and Swiss albino male mice in vivo

Ghosh, Manosij; Sinha, Shivam; Jothiramajayam, Manivannan; Jana, Aditi; Nag, Anish; Mukherjee, Anita

doi:10.1016/j.fct.2016.09.025

Cited by 70 publications

(42 citation statements)

References 44 publications

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“…For studying zinc oxide nanoparticles size and morphology, the aqueous dispersion of the nanoparticles was dropped cast onto a carbon-coated copper grid. This grid was air dried at room temperature [1] andvisualized using (Transmission Electron Microscope) (JEOL JEM-2100, Jeol Ltd, Tokyo, Japan) at Electron Microscope Research Unit, Faculty of Agriculture, ALmansora University, Egypt.…”

Section: Zinc Oxide Nanoparticle Characterizationmentioning

confidence: 99%

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Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Mesallam¹,

Deraz²,

Aal³

et al. 2019

J Histol Histopathol

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Background: Zinc oxide nanoparticles (ZnO NPs) have immense potential and application in various types of cosmetic products and health care sectors.They have attractive properties, and attracted great interest worldwide.Yet despite its potentially interesting beneficial effects, it has been proven that they can be also cytotoxic and genotoxic for multiple types of human cells, especially the reproductive system. Aim of the work: Our study aimed to detect the effect of oral subacute ZnO NPs on the testes and prostate of adult albino rats and the role of recovery. Materials and Methods: Forty adult male albino rats were divided into three groups; control (positive and negative), ZnONP-treated group for four weeks and a similarly treated group left for supposed recovery improvement testing two weeks after cessation of ZnONP administration. All rats were examined for serum oxidative stress markers, testicular and prostatic tissue inflammatory cytokines, DNA fragmentation, and extended histopthological evaluation.Histological study was performed using hematoxylin and eosin and Masson trichrome stains. Immunohistochemical expression for iNOs was performed. Morphometric and statistical analysis were also performed. Results: Statistically significant difference in the oxidative stress and inflammatory markers and DNA shearing and aggressive histopathological testicular and prostatic changes were also detected in ZnONPtreated group as compared to the control and the follow up group that showed significant improvement.. Immunohistochemical examination of testicular and prostatic sections of ZnONP-treated group revealed highly statistically significant increase in the mean area percent of iNOs immunoreactions which significantly decreased in follow up groups. Conclusion: ZnONPs have detrimental male reproductive effects that can be controlled by limiting the exposure to such products.

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Section: Zinc Oxide Nanoparticle Characterizationmentioning

confidence: 99%

“…They are found in many products such as sunscreens, food additives, pigments, rubber manufacture, and electronic materials. So, it was necessary to assess their safety as well as toxic aspects [1,2].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Mesallam¹,

Deraz²,

Aal³

et al. 2019

J Histol Histopathol

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“…23,24 The change in the oxidative status in BV-2 cells after being stimulated by ZnO NPs was evaluated using a DCFH-DA probe. The results …”

Section: Zno Nps Induced Oxidative Stress In Bv-2 Cellsmentioning

confidence: 99%

Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells

Wei

Wang

Chen

et al. 2017

IJN

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With the increasing application of zinc oxide nanoparticles (ZnO NPs) in biological materials, the neurotoxicity caused by these particles has raised serious concerns. However, the underlying molecular mechanisms of the toxic effect of ZnO NPs on brain cells remain unclear. Mitochondrial damage has been reported to be a factor in the toxicity of ZnO NPs. PINK1/parkin-mediated mitophagy is a newly emerging additional function of autophagy that selectively degrades impaired mitochondria. Here, a PINK1 gene knockdown BV-2 cell model was established to determine whether PINK1/parkin-mediated mitophagy was involved in ZnO NP-induced toxicity in BV-2 cells. The expression of total parkin, mito-parkin, cyto-parkin, and PINK1 both in wild type and PINK1 −/− BV-2 cells was evaluated using Western blot analysis after the cells were exposed to 10 μg/mL of 50 nm ZnO NPs for 2, 4, 8, 12, and 24 h. The findings suggested that the downregulation of PINK1 resulted in a significant reduction in the survival rate after ZnO NP exposure compared with that of control cells. ZnO NPs were found to induce the transportation of parkin from the cytoplasm to the mitochondria, implying the involvement of mitophagy in ZnO NP-induced toxicity. The deletion of the PINK1 gene inhibited the recruitment of parkin to the mitochondria, causing failure of the cell to trigger mitophagy. The present study demonstrated that apart from autophagy, PINK1/parkin-mediated mitophagy plays a protective role in ZnO NP-induced cytotoxicity.

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“…The CAs scored were mainly found to be chromatid breaks and the number of breaks per cell to be significantly higher than the control. About 10-fold increase in CAs was observed at the highest dose administered (25 mg/kg body weight) 30 . xenobiotic effects are reversible or not.…”

Section: Mammalian In Vivo Chromosomal Aberrations Assay (Ca)mentioning

confidence: 92%

Exploring the in vivo toxicity of nanoparticles

2017

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Toxicological tests of a xenobiotic play a key role to determine the safety of the new compound before it reaches the market. In this review article, we describe the main types of toxicological studies that can be performed in vivo to detect a possible undesired effect of a xenobiotic with especial emphasis on the data available for the different types of nanoparticles. The different procedures described in this review allow to obtain valuable information about the possible toxic effects of a xenobiotic to minimize the possible risks for patients once the compound has been approved for therapeutic use.

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Cyto-genotoxicity and oxidative stress induced by zinc oxide nanoparticle in human lymphocyte cells in vitro and Swiss albino male mice in vivo

Cited by 70 publications

References 44 publications

Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Toxicity of Subacute Oral Zinc Oxide Nanoparticles on Testes and Prostate of Adult Albino Rats and Role of Recovery

Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells

Exploring the in vivo toxicity of nanoparticles

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