Cytoarchitectonic and neurochemical differentiation of the visual system in ethanol-induced cyclopic zebrafish larvae

Santos-Ledo, Adrián; Arenzana, Francisco Javier; Porteros, A.; Lara, Juan M.; Velasco, Almudena; Aijón, José; Arévalo, R.

doi:10.1016/j.ntt.2011.06.001

Cited by 17 publications

(20 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the developing zebrafish retina only modest increases in cell death have been observed, when exposed to ethanol [18], [49]. The data presented in the current study shows significant increases in TUNEL-labeled cells in the neuromasts of zebrafish treated with ethanol.…”

Section: Discussioncontrasting

confidence: 38%

“…Additionally, these studies observed delayed differentiation in many retinal cell types including photoreceptors. Interestingly two studies saw either no difference or slight increases in proliferation in the ethanol-treated fish [18], [49]. Our data suggest yet another tissue-specific effect of ethanol on proliferation.…”

Section: Discussionmentioning

confidence: 42%

“…In both cases ethanol appears to alter cell cycle kinetics as well as change the proportion of cells that are actively cycling. Ethanol exposure during zebrafish retinal neurogenesis often results in reduced eye size and cyclopia at higher doses [16], [18], [49]. Additionally, these studies observed delayed differentiation in many retinal cell types including photoreceptors.…”

Section: Discussionmentioning

confidence: 96%

See 2 more Smart Citations

Ethanol Affects the Development of Sensory Hair Cells in Larval Zebrafish (Danio rerio)

2013

View full text Add to dashboard Cite

Children born to mothers with substantial alcohol consumption during pregnancy can present a number of morphological, cognitive, and sensory abnormalities, including hearing deficits, collectively known as fetal alcohol syndrome (FAS). The goal of this study was to determine if the zebrafish lateral line could be used to study sensory hair cell abnormalities caused by exposure to ethanol during embryogenesis. Some lateral line sensory hair cells are present at 2 days post-fertilization (dpf) and are functional by 5 dpf. Zebrafish embryos were raised in fish water supplemented with varying concentrations of ethanol (0.75%–1.75% by volume) from 2 dpf through 5 dpf. Ethanol treatment during development resulted in many physical abnormalities characteristic of FAS in humans. Also, the number of sensory hair cells decreased as the concentration of ethanol increased in a dose-dependent manner. The dye FM 1-43FX was used to detect the presence of functional mechanotransduction channels. The percentage of FM 1-43-labeled hair cells decreased as the concentration of ethanol increased. Methanol treatment did not affect the development of hair cells. The cell cycle markers proliferating cell nuclear antigen (PCNA) and bromodeoxyuridine (BrdU) demonstrated that ethanol reduced the number of sensory hair cells, as a consequence of decreased cellular proliferation. There was also a significant increase in the rate of apoptosis, as determined by TUNEL-labeling, in neuromasts following ethanol treatment during larval development. Therefore, zebrafish are a useful animal model to study the effects of hair cell developmental disorders associated with FAS.

show abstract

Section: Discussioncontrasting

confidence: 38%

Section: Discussionmentioning

confidence: 42%

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Ethanol Affects the Development of Sensory Hair Cells in Larval Zebrafish (Danio rerio)

2013

View full text Add to dashboard Cite

show abstract

“…Specifically, the gastrulation/neurulation transition period (8-10 hpf) and early nervous system development (24-27 hpf) have been shown to be sensitive to low levels (1.5 and 2%) of transient ethanol (resulting in eye and brain malformations) when agrin or Shh gene expression has been partially reduced using morpholino oligonucleotides (MOs) that disrupt agrin or Shh function (Zhang et al, 2013). More is known about structural changes to the CNS following longer durations of developmental ethanol exposure, including for example microphthalmia and/or cyclopia (exposed 0–72 hpf) (Bilotta et al, 2004; Dlugos and Rabin, 2007; Santos-Ledo et al, 2011) and increased cell death in the hindbrain (Carvan et al, 2004; Loucks and Carvan, 2004). …”

Section: Discussionmentioning

confidence: 99%

Long-term behavioral impairment following acute embryonic ethanol exposure in zebrafish

Bailey

Oliveri

Zhang

et al. 2015

Neurotoxicology and Teratology

View full text Add to dashboard Cite

BACKGROUND Developmental exposure to ethanol has long been known to cause persisting neurobehavioral impairment. However, the neural and behavioral mechanisms underlying these deficits and the importance of exposure timing are not well-characterized. Given the importance of timing and sequence in neurodevelopment it would be expected that alcohol intoxication at different developmental periods would result in distinct neurobehavioral consequences. METHODS Zebrafish embryos were exposed to ethanol (0%, 1%, 3%) at either 8-10 or 24-27 hours post-fertilization (hpf) then reared to adolescence and evaluated on several behavioral endpoints. Habituation to a repeated environmental stimulus and overall sensorimotor function were assessed using a tap startle test; measurements of anxiety and exploration behavior were made following introduction to a novel tank; and spatial discrimination learning was assessed using aversive control in a three-chambered apparatus. Overt signs of dysmorphogenesis were also scored (i.e. craniofacial malformations, including eye diameter and midbrain-hindbrain boundary morphology). RESULTS Ethanol treated fish were more active both at baseline and following a tap stimulus compared to the control fish and were hyperactive when placed in a novel tank. These effects were more prominent following exposure at 24-27 hpf than with the earlier exposure window, for both dose groups. Increases in physical malformation were only present in the 3% ethanol group; all malformed fish were excluded from behavioral testing. DISCUSSION These results suggest specific domains of behavior are affected following ethanol exposure, with some but not all of the tests revealing significant impairment. The behavioral phenotypes following distinct exposure windows described here can be used to help link cellular and molecular mechanisms of developmental ethanol exposure to functional neurobehavioral effects.

show abstract

“…α-acetylated Tubulin (Sigma T6793) staining was performed on sections after the in situ hybridization as previously described (31) and using an Alexa-488 secondary antibody.…”

Section: -Experimental Proceduresmentioning

confidence: 99%

Comparative gene expression analysis of the fmnl family of formins during zebrafish development and implications for tissue specific functions

Santos-Ledo¹,

Jenny²,

Marlow³

2013

Gene Expression Patterns

View full text Add to dashboard Cite

Fmlns belong to the Formin family, catalysts of linear Actin polymerization with mostly unknown roles in vivo. In cell culture Fmnls are involved in cell migration and adhesion and the formation of different types of protrusions including filopodia and blebs, suggesting important roles during development. Moreover, Fmnls can act downstream of Rac and Cdc42, mediators of cytoskeletal changes as targets of important pathways required for shaping tissues. The zebrafish genome encodes five Fmnls. Here we report their tissue specific expression patterns during early development and pharyngula stages. The fmnls show overlapping and distinct expression patterns, which suggest that they could regulate similar processes during development, but may also have independent functions. In particular, we find a strong maternal contribution of all fmnls, but distinct expression patterns in the developing brain eye, ear, heart and vascular system.

show abstract

Cytoarchitectonic and neurochemical differentiation of the visual system in ethanol-induced cyclopic zebrafish larvae

Cited by 17 publications

References 49 publications

Ethanol Affects the Development of Sensory Hair Cells in Larval Zebrafish (Danio rerio)

Ethanol Affects the Development of Sensory Hair Cells in Larval Zebrafish (Danio rerio)

Long-term behavioral impairment following acute embryonic ethanol exposure in zebrafish

Comparative gene expression analysis of the fmnl family of formins during zebrafish development and implications for tissue specific functions

Contact Info

Product

Resources

About