Cytochalasin E in the lichen Pleurosticta acetabulum . Anti-proliferative activity against human HT-29 colorectal cancer cells and quantitative variability

Background: Oxidative stress is defined as imbalance between oxidant and antioxidant ratio, that lead to oxidative damage of biologically active molecules, finally lead to different disease and initiate carcinogenesis. The drug discovery using natural products as medicinal plants or marine organism still an important target for recent research. This study investigated anticancer activity of algae extract obtained from Red sea at Jeddah. Materials and Methods: Aqueousand methanol extracts ofDictyotaciliolata(DC) were tested on HCT-116 and HepG2 cell lines using WST-1. Aqueous extract (AEDC) and methanol extract (MEDC) at doses 0.05, 0.1, 0.5, 1 mg/ml and positive control 0.3% H2O2 at doses 0.5 mg/ml for 24,48 and 72 h (for two cell lines). Results: AEDC showed the mosteffective antitumor activity against HCT116 and HePG2, the IC50 dose for HCT116 cells was 0.05 mg/ml at 72 h, while for the HePG2 it was 0.01 mg/ml at 72 h. These results showed that HePG2cells was more sensitive to the AEDC. However, IC50 for MEDC were 0.01 mg/ml for HCT116 and 0.05 for HepG2 at 48 Hrs. The algae extracts contain sulfated polysaccharides and different pigments as chlorophylls, carotenoids and phycobiliproteins. These pigments were approved as biological active biomolecules that exert different biological activities as antioxidants, antitumor and rich source of micronutrients. In addition, AEDC or MEDC exert apoptotic activity by increase activity of caspase 3 and 9 in HepG2. Conclusıon: The antitumor effect of AEDC or MEDC is promising for development of chemotherapeutic agent as effective with no side effects.

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“…These systems include (a) endogenous, some antioxidants produced in the body and (b) exogenous, obtained from diet. [11].…”

Section: Resultsmentioning

confidence: 99%

In vitro Study Anti-proliferative Potential of Algae Extract against Cancer Cell Line

Maddah

Huwait

Al-Balawi

et al. 2019

JPRI

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“…The presence of all families of metabolites in the apothecia suggests that sexual reproductive parts in P. crocata are highly protected against lichenophages. Various examples of an accumulation of specialized metabolites in parts of lichens with high fitness value as reproductive parts were described, including parietin, physodic acid, atranorin, pinastric acid, m-scrobiculin, and cytochalasin [10,[43][44][45]. However, accumulation of metabolites in reproductive parts is not the rule for all metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Pulvinic acid derivatives (including calycin) are well known powerful anti-lichenophages [48,50] and their detection in the reproductive parts of P. crocata highlights an optimal defense strategy for soralia [10,43]. In contrast to other snails [45], N. hookeri was able to eat calycin-containing soralia because their characteristic yellow pigment was retrieved in the snail feces, although calycin was the highest repellent compound in starch gels. Conversely, stictic acid was absent from the digestive tract and feces of the snail N. hookeri [22], but it was sparsely consumed in starch gels.…”

Section: Discussionmentioning

confidence: 99%

Mass Spectrometry Imaging of Specialized Metabolites for Predicting Lichen Fitness and Snail Foraging

Gadea

Fanuel

Lamer

et al. 2020

Plants

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Lichens are slow-growing organisms supposed to synthetize specialized metabolites to protect themselves against diverse grazers. As predicted by the optimal defense theory (ODT), lichens are expected to invest specialized metabolites in higher levels in reproductive tissues compared to thallus. We investigated whether Laser Desorption Ionization coupled to Mass Spectrometry Imaging (LDI-MSI) could be a relevant tool for chemical ecology issues such as ODT. In the present study, this method was applied to cross-sections of thalli and reproductive tissues of the lichen Pseudocyphellaria crocata. Spatial mapping revealed phenolic families of metabolites. A quantification of these metabolites was carried out in addition to spatial imaging. By this method, accumulation of specialized metabolites was observed in both reproductive parts (apothecia and soralia) of P. crocata, but their nature depended on the lichen organs: apothecia concentrated norstictic acid, tenuiorin, and pulvinic acid derivatives, whereas soralia mainly contained tenuiorin and pulvinic acid. Stictic acid, tenuiorin and calycin, tested in no-choices feeding experiments, were deterrent for N. hookeri while entire thalli were consumed by the snail. To improve better knowledge in relationships between grazed and grazing organisms, LDI-MSI appears to be a complementary tool in ecological studies

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“…Extract of Pleurosticta acetabulum exerted strong cytotoxic effect on HT-29 cells. This extract also inhibited the proliferation of HT-29 cells through the presence of cytochalasin E and induced apoptosis [110]. Furthermore, polysaccharide from Umbilicaria esculenta decreased the viability of human melanoma A875 and A375 cells but did not exhibit any cytotoxic effect on HUVEC cells.…”

Section: Determination Of Lichens' Extracts Effect Against Cancer Cellsmentioning

confidence: 90%

Anticancer Potential of Lichens’ Secondary Metabolites

et al. 2020

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Lichens produce different classes of phenolic compounds, including anthraquinones, xanthones, dibenzofuranes, depsides and depsidones. Many of them have revealed effective biological activities such as antioxidant, antiviral, antibiotics, antifungal, and anticancer. Although no clinical study has been conducted yet, there are number of in vitro and in vivo studies demonstrating anticancer effects of lichen metabolites. The main goal of our work was to review most recent published papers dealing with anticancer activities of secondary metabolites of lichens and point out to their perspective clinical use in cancer management.

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Cytochalasin E in the lichen Pleurosticta acetabulum . Anti-proliferative activity against human HT-29 colorectal cancer cells and quantitative variability

Cited by 21 publications

References 24 publications

In vitro Study Anti-proliferative Potential of Algae Extract against Cancer Cell Line

In vitro Study Anti-proliferative Potential of Algae Extract against Cancer Cell Line

Mass Spectrometry Imaging of Specialized Metabolites for Predicting Lichen Fitness and Snail Foraging

Anticancer Potential of Lichens’ Secondary Metabolites

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