1983
DOI: 10.1002/aja.1001680207
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Cytochemical analysis of alkaline phosphatase and esterase activities and of lectin‐binding and anionic sites in rat and mouse Peyer's patch M cells

Abstract: M cells in Peyer's patch follicle epithelium endocytose and transport luminal materials to intraepithelial lymphocytes. We examined (1) enzymatic characteristics of the epithelium covering mouse and rat Peyer's patches by using cytochemical techniques, (2) distribution of lectin-binding sites by peroxidase-labeled lectins, and (3) anionic site distribution by using cationized ferritin to develop a profile of M cell surface properties. Alkaline phosphatase activity resulted in deposits of dense reaction product… Show more

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Cited by 167 publications
(78 citation statements)
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References 45 publications
(40 reference statements)
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“…Because both oropharynx and cecum are heavily populated by bacteria (Miller, 1990;Hill and Drasar, 1975), the M-cell-specific glycoconjugates might represent adaptations to the local microbial flora that allow the selective uptake of microbial antigens for the initiation of immunological tolerance against these bacteria. This view is supported by the absence of M-cell-specific glycoconjugates in the small intestine of rabbits and rats (Jepson et al, 1995;Gebert and Hach, 1993;Owen and Bhalla, 1983) -a habitat in which the indigenous microbial flora probably plays a minor role (Savage, 1977). In addition, the cecum of mice, which normally contains large numbers of indigenous bacteria (Koopman et al,l983), bears M-cells that considerably differ in their lectin binding pattern from those in the small intestinal Peyer's patches (Clark et al, 1994(Clark et al, ,1995Giannasca et al, 1994).…”
Section: Discussionsupporting
confidence: 50%
“…Because both oropharynx and cecum are heavily populated by bacteria (Miller, 1990;Hill and Drasar, 1975), the M-cell-specific glycoconjugates might represent adaptations to the local microbial flora that allow the selective uptake of microbial antigens for the initiation of immunological tolerance against these bacteria. This view is supported by the absence of M-cell-specific glycoconjugates in the small intestine of rabbits and rats (Jepson et al, 1995;Gebert and Hach, 1993;Owen and Bhalla, 1983) -a habitat in which the indigenous microbial flora probably plays a minor role (Savage, 1977). In addition, the cecum of mice, which normally contains large numbers of indigenous bacteria (Koopman et al,l983), bears M-cells that considerably differ in their lectin binding pattern from those in the small intestinal Peyer's patches (Clark et al, 1994(Clark et al, ,1995Giannasca et al, 1994).…”
Section: Discussionsupporting
confidence: 50%
“…This is referred to as follicle-associated epithelium (FAE) and is a site of active immunological function. In contrast to villous epithelium, FAE contains no or fewer goblet cells (67), defensin-and lysozyme-producing Paneth cells (26,27) and expresses low amounts of membrane-associated hydrolases (68). The M cells generally lack the distinct microvilli and thick filamentous brush border glycocalyx (24) and instead have variable microfolds.…”
Section: Is Important For the Inhibition Of Phagocytosis By Epec And mentioning
confidence: 99%
“…Antigenic substances such as pathogenic microorganisms are eliminated by nonspecific and specific defense mechanisms mediated by gutassociated lymphatic tissue (GALT) [11,34]. The follicleassociated epithelium (FAE) of Peyer's patches contains M cells which play special functions that transcytose various antigens and microorganisms from the intestinal lumen to the dome area [23,27]. Some antigens are further delivered to the antigen-presenting cells to induce the mucosal immune responses [14,25].…”
mentioning
confidence: 99%