1980
DOI: 10.1172/jci109794
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Cytochemical Bioassay of Parathyroid Hormone

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Cited by 143 publications
(7 citation statements)
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“…This effect is not observed with the serotonin receptor, which is known to utilize the InsP 3 pathway, and it thus provides further evidence that InsP 3 may not be the signal for the PTH system. It is of interest that the most sensitive assay for PTH is based on a procedure that requires activation of glucose-6-phosphate dehydrogenase and production of NADPH (52). The biochemical rationale for this assay is not understood, but our results with NADP ϩ now provide further impetus for examination of the possible role of this coenzyme in biochemical actions of PTH.…”
Section: Discussionmentioning
confidence: 96%
“…This effect is not observed with the serotonin receptor, which is known to utilize the InsP 3 pathway, and it thus provides further evidence that InsP 3 may not be the signal for the PTH system. It is of interest that the most sensitive assay for PTH is based on a procedure that requires activation of glucose-6-phosphate dehydrogenase and production of NADPH (52). The biochemical rationale for this assay is not understood, but our results with NADP ϩ now provide further impetus for examination of the possible role of this coenzyme in biochemical actions of PTH.…”
Section: Discussionmentioning
confidence: 96%
“…Citratemia analysis was conducted using a chromatography ionic test using AG CH9101 columns and then detected by conductimetry with the BIORAD® Automatic Analyzer [15]. Intact parathyroid hormone was determined by automated immunoassay with the ARCHITECT I System (Abbott, Germany) using the ARCHITECT Inteact PTH Reagent Kit (Abbott) [16]. …”
Section: Methodsmentioning
confidence: 99%
“…The cytochemical bioassay was performed as described (14,18). Guinea pig renal cortical segments were cultured with or without graded doses of hormone standard (50 fg/ml to 50 pg/ml) or Antiserum preincubation studies were performed with antiserum G-5, a goat anti-human PTH antiserum that reacts with both amino-and carboxyl-terminal portions of the PTH sequence (19).…”
mentioning
confidence: 99%