Cytochemical localization of fructose-1,6-bisphosphatase in Thiobacillus neapolitanus carboxysomes

Beudeker, R.F.; Veenhuis, Marten; Kuenen, J.G.

doi:10.1016/0378-1097(81)90215-9

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“…The association of RuBisCO with carboxysomes has also been demonstrated for other autotrophs, including colourless sulphur-, nitrite-and ammoniaoxidizing bacteria . The possibility was raised that other Calvin cycle enzymes may also be located in carboxysomes and evidence for, and against, this concept has been obtained for Thiobacillus neapolitanus (Beudeker & Kuenen, 198 1 ;Beudeker et al, 198 1 ;Cannon & Shively, 1983). Our interest in the location of the second enzyme essential for and unique to the Calvin cycle, phosphoribulokinase (PRK), was prompted by the presence in dissociated C .…”

Section: Introductionmentioning

confidence: 99%

Immunoelectronmicroscopic Localization of Calvin Cycle Enzymes in Chlorogloeopsis fritschii

1985

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Antisera against the Calvin cycle enzymes D-ribulose-l,5-bisphosphate carboxylase/oxygenase (RuBisCO) and phosphoribulokinase (PRK) have been used in immunogold electronmicroscopy studies on cell sections of the cyanobacterium Chlorogloeopsis fritschii. RuBisCO antiserum consistently labelled the carboxysomes (polyhedral bodies) and the cytoplasm. PRK antiserum-labelling occurred in the cytoplasm but not in the carboxysomes. The data agree with in vitro enzyme localization studies, and confirm that both enzymes occur in the cytoplasm and that RuBisCO, but not PRK, also occurs in the carboxysomes of C. jritschii. METHODS Organism and growth. Chlorogloeopsis jritschii 141 1/16, from the Culture Centre of Algae and Protozoa, Cambridge, UK, was grown as detailed before (Codd & Stewart, 1973), except that cells were harvested during early stationary phase. Celljxation. Cells were harvested by centrifugation at 2000g for 10 min and fixed in phosphate buffer (0.1 M, pH 7.2) containing 2% (v/v) glutaraldehyde for 2-6 h at room temperature. Excess glutaraldehyde was removed by

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Section: Introductionmentioning

confidence: 99%