Cytochemical localization of reactive oxygen species (O2− and H2O2) and peroxidase in the incompatible and compatible interaction of wheat – Puccinia striiformis f. sp. tritici

Wang, Chen-Fang; Huang, Lili; Zhang, Hongchang; Han, Qingmei; Buchenauer, H.; Kang, Zhensheng

doi:10.1016/j.pmpp.2010.02.002

Cited by 30 publications

(17 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection of H 2 O 2 was performed according to the method of Bestwick et al (1997) and Wang et al (2010) , Mn 2+ is oxidized to Mn 3+ which then oxidizes 3′,3′-diaminobenzidine HCl (DAB) to an insoluble osmiophilic polymer. Tissue blocks as described above were incubated for 30 min in 0.1 M HEPES buffer (pH 7.2) containing 2.5 mM DAB, 0.5 mM MnCl 2 , and 1 mM Na-azide.…”

Section: Ultracytochemical Localization Of H 2 Omentioning

confidence: 99%

“…Elevated levels of ROS can lead to the lipid peroxidation of cell membranes which increases cytomembrane permeability and ion leakage (Apel and Hirt 2004;Fryer et al 2002). Under the biotic stress, a large amount of H 2 O 2 and O 2 − accumulates in the cell walls, cytomembranes, intercellular spaces, and mitochondria of wheat leaf cells which causes the oxidative damage (Bestwick et al 1997;Wang et al 2010). ROS drive the oxidation of mitochondria and release of cytochrome c which induce the PCD (Casolo et al 2005;Chen et al 2009;Yao et al 2004).…”

Section: Ros Affect On the Pcd Under Waterlogging Treatment In Wheat mentioning

confidence: 99%

See 1 more Smart Citation

Reactive oxygen species regulate programmed cell death progress of endosperm in winter wheat (Triticum aestivum L.) under waterlogging

Cheng

Zhang

et al. 2015

Protoplasma

View full text Add to dashboard Cite

Previous studies have proved that waterlogging stress accelerates the programmed cell death (PCD) progress of wheat endosperm cells. A highly waterlogging-tolerant wheat cultivar Hua 8 and a waterlogging susceptible wheat cultivar Hua 9 were treated with different waterlogging durations, and then, dynamic changes of reactive oxygen species (ROS), gene expressions, and activities of antioxidant enzymes in endosperm cells were detected. The accumulation of ROS increased considerably after 7 days of waterlogging treatment (7 DWT) and 12 DWT in both cultivars compared with control group (under non-waterlogged conditions), culminated at 12 DAF (days after flowering) and reduced hereafter. Waterlogging resulted in a great increase of H2O2 and O2 (-) in plasma membranes, cell walls, mitochondrias, and intercellular spaces with ultracytochemical localization. Moreover, the deformation and rupture of cytomembranes as well as the swelling and distortion of mitochondria were obvious. Under waterlogging treatment conditions, catalase (CAT) gene expression increased in endosperm of Hua 8 but activity decreased. In addition, Mn superoxide dismutase (MnSOD) gene expression and superoxide dismutase (SOD) activity increased. Compared with Hua 8, both CAT, MnSOD gene expressions and CAT, SOD activities decreased in Hua 9. Moreover, ascorbic acid and mannitol relieve the intensifying of PCD processes in Hua 8 endosperm cells induced by waterlogging. These results indicate that ROS have important roles in the PCD of endosperm cells, the changes both CAT, MnSOD gene expressions and CAT, SOD activities directly affected the accumulation of ROS in two different wheat cultivars under waterlogging, ultimately led to the PCD acceleration of endosperm.

show abstract

Section: Ultracytochemical Localization Of H 2 Omentioning

confidence: 99%

Section: Ros Affect On the Pcd Under Waterlogging Treatment In Wheat mentioning

confidence: 99%

Reactive oxygen species regulate programmed cell death progress of endosperm in winter wheat (Triticum aestivum L.) under waterlogging

Cheng

Zhang

et al. 2015

Protoplasma

View full text Add to dashboard Cite

show abstract

“…The inactivation of cell wall peroxidases through azide and cyanide and the inactivation of plasma membrane NADPH oxidases through diphenylene iodonium (DPI) revealed that different factors might be involved in various pathosystems, increasing complexity in a system de Gara et al 2003;Wang et al 2010). There is also evidence of a lack of correlation between NADPH oxidase and the production of ROS (Glazener et al 1996;Torres et al 2006), suggesting that we still do not fully understand the process or processes that establish the character of plant-pathogen interactions and the role of ROS in hypersensitive response (HR) (Shetty et al 2007).…”

mentioning

confidence: 99%

The origin of reactive oxygen during interaction of Pinus sylvestris root and Heterobasidion annosum s.l. – the linkage with the iron

et al. 2015

View full text Add to dashboard Cite

Reactive oxygen species (ROS) can be promoted differently, although iron is regarded as a condicio sine qua of their generation. We assessed how enzymes involved in ROS production and iron accumulation impact the interactions between Pinus sylvestris root and p at ho g e ns w i t h di ff e r e n t ho s t pr ef e r e n ce s (Heterobasidion annosum sensu stricto, H. parviporum and H. abietinum). Studies with inhibitors of ROS generation showed that diphenylene iodonium (DPI) and potassium cyanide (KCN) decreased the mortality of P. sylvestris root cells when exposed to H. parviporum in the elongation zone. In the meristematic zone, inhibited production of NADPH oxidase and oxalic acid decreased mortality of host root cells challenged with H. parviporum or H. abietinum. In the meristematic zone, iron immobilization with desferoxamine (DFO), inhibited percentage of dead host cells exposed to H. annosum s.s. Scots pine inoculation with high compatible H. annosum s.s., irrespectively in the root zone, led to an accumulation of superoxides (O 2 − ) and a colocalisation of H 2 O 2 with ferric or ferrous ions involved in production of the most harmful ROS -hydroxyl radicals at 4 h. After 48 h of mutual interaction between P. sylvestris and H. annousum s.s, a similar pattern as at 4 h was found in the root meristematic zone. We also showed that O 2 − accumulation in the P. sylvestris -H. annosum s.s. pathosystem was not NADPH oxidase dependent. During the interaction of P. sylvestris and H. annosum s.s., colocalisation of H 2 O 2 with Fe 2+ and Fe 3+ decreased by disturbing ROS production; this pattern was less apparent when H. parviporum or H. abietinum were identified as the invader. Our work implies that different mechanisms related to plant cell death can operate in both the root elongation and meristematic zone of P. sylvestris colonized with H. annosum s.s. pathosystem.

show abstract

“…The full‐length cDNA sequence was isolated from cDNA of wheat cv. Suwon 11, which is highly susceptible to virulent Pst race CYR31 and highly resistant to avirulent Pst race CYR23 (Wang et al ). The predicted ORF of TaMDAR6 spans 1458 bp and encodes a protein of 485 amino acids with a predicted molecular mass of 52.04 kDa.…”

Section: Resultsmentioning

confidence: 99%

TaMDAR6 acts as a negative regulator of plant cell death and participates indirectly in stomatal regulation during the wheat stripe rust–fungus interaction

Abou-Attia

Wang

Al-Attala

et al. 2015

Physiologia Plantarum

View full text Add to dashboard Cite

We identified a new monodehydroascorbate reductase (MDAR) gene from wheat, designated TaMDAR6, which is differentially affected by wheat-Puccinia striiformis f. sp. tritici (Pst) interactions. TaMDAR6 is a negative regulator of plant cell death (PCD) triggered by the Bax gene and Pst. Transcript levels of TaMDAR6 are significantly upregulated during a compatible wheat-Pst interaction, indicating that TaMDAR6 may contribute to plant susceptibility. In addition, H2 O2 production and PCD are significantly induced and initial pathogen development is significantly reduced in the TaMDAR6 knocked-down plants upon Pst infection. Thus, the suppression of TaMDAR6 enhances wheat resistance to Pst. Besides, the suppression of TaMDAR6 during an incompatible interaction induces a change in the morphology of stomata, which leads to poor stoma recognition and as a consequence to reduced infection efficiency. The percentage of infection sites that develop substomatal vesicles decreases in the TaMDAR6 knocked-down plants during the incompatible interaction presumably due to the increase in ROS accumulation, which is likely to activate other resistance mechanisms that have a negative effect on substomatal vesicle formation. TaMDAR6 can therefore be considered a negative regulator of PCD and of wheat defense to Pst.

show abstract

Cytochemical localization of reactive oxygen species (O2− and H2O2) and peroxidase in the incompatible and compatible interaction of wheat – Puccinia striiformis f. sp. tritici

Cited by 30 publications

References 34 publications

Reactive oxygen species regulate programmed cell death progress of endosperm in winter wheat (Triticum aestivum L.) under waterlogging

Reactive oxygen species regulate programmed cell death progress of endosperm in winter wheat (Triticum aestivum L.) under waterlogging

The origin of reactive oxygen during interaction of Pinus sylvestris root and Heterobasidion annosum s.l. – the linkage with the iron

TaMDAR6 acts as a negative regulator of plant cell death and participates indirectly in stomatal regulation during the wheat stripe rust–fungus interaction

Contact Info

Product

Resources

About