Cytochemical staining of a yeast polyphosphate fraction, localized outside the plasma membrane

Tijssen, J.P.F.; Steveninck, J. Van; Bruijn, W. C. de

doi:10.1007/bf01297357

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…In contrast to all previous studies on poly P in fungal cell walls, we employed speciWc PBPs to localize poly P in the cell wall. Other studies on poly P in fungal cell walls either analyzed isolated cell walls or performed electron microscopy (Datema et al, 1977;Harold, 1962;Shari'a et al, 2002;Tijssen et al, 1985). The major limitation for the unmistakable localization of poly P in isolated fungal cell walls are the huge poly P stores that occur in almost all fungi intracellularly.…”

Section: Discussionmentioning

confidence: 99%

“…A high poly P content was found in cell wall preparations of various zygomycetes (Datema et al, 1977), and its presence was inferred from electron microscopical observation after staining with osmium tetroxide or lead (Shari'a et al, 2002;Tijssen et al, 1985). Poly P has also been reported for the cell envelope of the ascomycetes Neurospora crassa, Endomyces magnusii (Kulaev et al, 2004) and Kluyveromyces marxianus (Tijssen et al, 1985).…”

Section: Introductionmentioning

confidence: 96%

“…Poly P has also been reported for the cell envelope of the ascomycetes Neurospora crassa, Endomyces magnusii (Kulaev et al, 2004) and Kluyveromyces marxianus (Tijssen et al, 1985). However, since fungi generally contain high intracellular poly P pools, concern was raised that in all these cases poly P might have bound to the cell wall as an artefact during preparation or Wxation (Datema et al, 1977;Harold, 1962).…”

Section: Introductionmentioning

confidence: 97%

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Specific localization of inorganic polyphosphate (poly P) in fungal cell walls by selective extraction and immunohistochemistry

Werner

Amrhein

Freimoser

2007

Fungal Genetics and Biology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Specific localization of inorganic polyphosphate (poly P) in fungal cell walls by selective extraction and immunohistochemistry

Werner

Amrhein

Freimoser

2007

Fungal Genetics and Biology

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“…Staining with Pb(NO,), can only detect large aggregates (Fig. 4a) and others may be solubilized and washed out during the preparation steps for conventional electron microscopy (Tijssen et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

Identification of structures containing polyphosphate in Helicobacter pylori

Bode

Mauch

Ditschuneit

et al. 1993

Journal of General Microbiology

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For the first time polyphosphate (poly P) granules have been detected in Helicobacter pylori organisms colonizing the gastric antrum as well as in organisms isolated from the same tissue. Poly P granules showed typical sublimation characteristics during exposure to the electron beam and chipped out of ultrathin sectioning. A prominent phosphorus signal was identified using elemental specific electron microscopy such as electron energy loss spectroscopy (EELS) and was localized to at least three different locations: the cytoplasm, the flagellar pole and in association with the cell membrane. Intracytoplasmatic structures had a diameter of 0.05-0.2 pm, whereas the structures near the flagellar pole were much smaller (0-02 pm). The membrane-associated phosphate aggregates were visible only after staining with Pb(NO,), or with electron spectroscopic imaging (ESI). Poly P granules seem to be important energy and phosphorus stores and it is thought that they participate in the regulation of various and distinct metabolic processes of H. pylori.

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“…Lead staining results in better visualization of polyphosphates (Tijssen & van Steveninck, 1984), but its use would have complicated the interpretation of the X-ray spectra by generating a peak close to that of P, possibly masking it. During preparation for conventional electron microscopy, phosphates may also be solubilized and washed out (Tijssen, van Steveninck & De Bruijn, 1985). Moreover, the diameter ofthe Xray probe (200 nm) is also a limiting factor for very fine and precise localization.…”

Section: Phosphorylated Fractionsmentioning

confidence: 99%

Polyphosphates in the red macroalga Chondrus crispus (Rhodophyceae)

1997

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SUMMARYPlants of Chondrus crispus Stackhouse, collected from mid-littoral tidepools, were treated as follows. Some plants were kept for a few weeks under controlled starvation conditions in order to decrease their initial content of total tissue phosphorus, then incubated for up to 48 h in phosphorus (15 /^M) and nitrogen (25 /iM)enriched sea water. Other plants were directly incubated in enriched sea water. Chemical analyses showed that the total phosphorus content of fresh and starved plants remained stable, reflecting the nutritional status of the plants. The predominant acid-soluble phosphate fraction was larger in fresh than in starved plants. The content of acid-soluble polyphosphates, similar in both types of plants at the beginning of the experiment, doubled in starved plants, and increased by a factor of 2-7 in fresh plants, over 48 h. The content of acid-insoluble polyphosphates was lower than that of acid-soluble polyphosphates.Transmission electron microscopy and energy dispersive X-ray microanalysis confirmed the presence, mostly in medullary cells, of acid-insoluble polyphosphates in the form of cytoplasmic granules and precipitates along the plasmalemma, particularly near pit plugs. This is the first report of such phosphorus storage structures in a red macroalgal species.

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Cytochemical staining of a yeast polyphosphate fraction, localized outside the plasma membrane

Cited by 17 publications

References 22 publications

Specific localization of inorganic polyphosphate (poly P) in fungal cell walls by selective extraction and immunohistochemistry

Specific localization of inorganic polyphosphate (poly P) in fungal cell walls by selective extraction and immunohistochemistry

Identification of structures containing polyphosphate in Helicobacter pylori

Polyphosphates in the red macroalga Chondrus crispus (Rhodophyceae)

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