Cytochemical Studies of Nucleic Acids and Proteins in Erythrocytic Development

Grasso, Joseph A.; Woodard, John; Swift, Hewson

doi:10.1073/pnas.50.1.134

Cited by 93 publications

(49 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By 36 h, when the erythroid cell population consisted mainly of intermediate and late stage normoblasts, nuclear RNA polymerase activity had declined to control levels. The decrease in polymerase activity is consistent with the morphologic changes (reduction in cytoplasmic ribosomes, condensation of nuclear chromatin) which are observed in erythroblasts in the later stages of maturation (14).…”

Section: Methodssupporting

confidence: 62%

Sequential activation of splenic nuclear RNA polymerases by erythropoietin.

Piantadosi

Dickerman

Spivak³

1976

J. Clin. Invest.

View full text Add to dashboard Cite

Section: Methodssupporting

confidence: 62%

Sequential activation of splenic nuclear RNA polymerases by erythropoietin.

Piantadosi

Dickerman

Spivak³

1976

J. Clin. Invest.

View full text Add to dashboard Cite

“…Dashed line, radioactivity ; solid line, optical density at 280 mµ of the marker adult hemoglobin . previously reported (Grasso et al ., 1963 ;Djaldetti et al ., 1970), polychromatophilic erythroblasts are more active in protein synthesis than orthochromatic erythroblasts and this relationship is not altered after 24 hr culture either with or without the hormone. Moreover, the moderate fall in isotope incorporation which occurs in cells of both developmental stages after culture is independent of the presence or absence of the hormone .…”

Section: Hemoglobin Synthesismentioning

confidence: 77%

“…Since hemoglobin is the major protein synthesized by these cells, these data suggest that the hormone-induced increase in hemoglobin synthesis per culture does not reflect an increase in the rate of synthesis of hemoglobin per polychromatophilic or orthochromatic erythroblasts. Most of the hemolgobin synthesis which occurs in the erythroid cell population in culture is accomplished by cells of these DISCUSSION stages whereas protein synthesis by proerythroblasts and basophilic erythroblasts consists principally of nonhemoglobin proteins (Thorell, 1947 ;Grasso et al ., 1963 ;Djaldetti et al ., 1970) .…”

Section: Thymidine-1 H Labeling Indexmentioning

confidence: 99%

Erythropoietin Effects on Fetal Mouse Erythroid Cells

Chui

Djaldetti

Marks

et al. 1971

The Journal of Cell Biology

View full text Add to dashboard Cite

The effect of the hormone, erythropoietin, on cultures of erythroblasts derived from the livers of fetal C57BL/6J mice was examined . An increase both in the content and in the rate of synthesis of normal adult mouse globin chains was detected in hormone-treated cultures . The rate of protein synthesis by individual erythroblasts does not increase in response to the hormone, whereas the absolute number of hemoglobin-synthesizing cells does increase and accounts for the observed stimulation of hemoglobin synthesis . The principal effect of erythropoietin appears to be upon the population of immature erythroid precursor cells which persists in the presence of the hormone, the cells maintaining their ability to replicate, and their capacity to differentiate into hemoglobinizing erythroblasts . In the absence of hormone, already committed erythroblasts continue their development, but erythropoiesis is not sustained .

show abstract

“…This relative insensitivity of erythroblast protein synthesis to the antibiotic's virtually complete curtailment of RNA production suggests that reutilization of messenger RNA's, and perhaps ribosomes also, is more extensive in these cells than in lymphocytes . Stable messenger RNA is also a feature of erythrocyte development in amphibians (3), birds (13), and mammals (14,15) .…”

Section: Rna and Protein Synthesismentioning

confidence: 99%