Cytochemistry of eimeria brunetti and E. Acervulina of the chicken

SYNOPSIS. Monolayer primary and secondary cultures of embryonic bovine kidney, spleen, intestinal and testicle cells, and secondary cultures of embryonic bovine thymus, maintained in lactalbumin hydrolysate, Earle's balanced salt solution and ovine serum were observed for a maximum of 21 days after inoculation of E. bovis sporozoites. The sporozoites entered the cells in all of these cultures but underwent development only in primary cultures of kidney and intestinal cells and in secondary cultures of kidney, spleen, thymus, intestinal, and testicle cells. In acellular media, the sporozoites retained motility no longer than 21 hr. In the cell cultures, free motile sporozoites were seen for as long as 18 days after inoculation. Sporozoites entered cells anterior end first; the process of penetration required a few seconds to about a minute. Sporozoites were also observed leaving host cells. Intracellular sporozoites were first seen 3 min after inoculation; they were observed at various intervals up to 18 days after inoculation. In transformation of sporozoites into trophozoites a marked change in size and appearance of the nucleus took place before the change in shape of the body occurred. Trophozoites were first found 7 days after inoculation, multinucleate schizonts after 8 days, and schizonts with merozoites after 14 days. Schizonts containing merozoites were seen only in kidney, spleen, and thymus cells. The mature schizonts were smaller and represented a much lower proportion of the total number than in comparable stages of infections in calves. Schizonts with many nuclei occurred in intestinal cells; the most advanced stage seen in testicle cells was the binucleate schizont. Nuclear and cytoplasmic changes were observed in the infected cells.

Section: Discussionmentioning

confidence: 78%

Development of First‐Generation Schizonts of Eimeria bovis in Cultured Bovine Cells

Fayer

Hammond

1967

“…Obviously they are lipids. Lipid droplets in these stages can be demonstrated under the light microscope following staining with Sudan 111 (3,7,11). The lipid inclusions of the macrogametocytes have an irregular shape as seen in electron micrographs (Figs.…”

Section: Resultsmentioning

confidence: 99%

Electron Microscope Studies on Eimeria perforans (Sporozoa)

Scholtyseck¹

1962

SYNOPSIS. By means of electron microscopy, a study has been made of the fine structure of the macrogametocytes, microgametocytes and oocysts of Eimeria perforans from the intestine of the wild rabbit (Oryctolagus cuniculus). The parasites lie in a vacuole within the host cell. The surface of the gametocytes is not plain, but displays irregular protrusions. A large intranuclear body can be detected within the macrogametocytes. Similar structures are also found within the cytoplasm. Within the latter there exists a large spread out reticulum, the channels and vesicles of which concentrate especially close to the nuclear membrane. Tubuli are seen in the numerous mitochondria, which often have a dumb‐bell shape. In most of the gametocytes irregular, strongly osmiophilic lipid inclusions are observed, which always are surrounded by the endoplasmic reticulum. Strange folded ovoid bodies are found within the cytoplasm of the oocysts. Nothing can be told with certainty of their nature and function. Probably they represent specific storage bodies.

“…were collected at the time of their first massive discharge into the chick intestine, and isolated in the cold by means of continuous centrifugal flotation in high-density media. Maximum numbers (90%) of sporoblastic oocysts were obtained by incubating a t 30°C for 10 hr and sporulation was considered complete within 20 hr. The initial Qo?…”

Section: Synopsis Oocysts Of Eimeria Acervulinamentioning

confidence: 99%

“…A 15% decrease in dry weight in 15 hr mas due chiefly to disappearance of alkali-stable carbo-PORULATION in oocysts of Eimeria spp., which S takes place outside the host and in which no exchanges with the environment are known to occur with the exception of respiratory gases(23), has been studied in considerable cytochemical detail (1)(2)(3)5,6,(9)(10)(11)17,20,21). Pattillo & Becker (20) detected large amounts of carbohydrate, which they assumed to be glycogen, in macrogametocytes and unsporulated oocysts of Einzeria aceraulina, less in sporulated oocysts. and none in excysted sporozoites.…”

Section: Synopsis Oocysts Of Eimeria Acervulinamentioning

confidence: 99%

Biochemistry of Sporulation in Oocysts of Eimeria acervulina*

Wilson

Fairbairnz

1961

Oocysts of Eimeria acervulina Tyzzer, 1929 were collected at the time of their first massive discharge into the chick intestine, and isolated in the cold by means of continuous centrifugal flotation in high‐density media. Maximum numbers (90%) of sporoblastic oocysts were obtained by incubating at 30°C for 10 hr and sporulation was considered complete within 20 hr. The initial Qo2 (dry‐weight basis) of 9.5 decreased linearly to < i/3 this value in 20 hr, and more slowly thereafter to very low levels. The QCO2 was higher than Qo2 only during the first 10 hr. A 15% decrease in dry weight in 15 hr was due chiefly to disappearance of alkali‐stable carbohydrate, probably identical with glycogen. In the later stages carbohydrate was resynthesized. Lipids were not oxidized during sporoblast formation, but disappeared rapidly betweem 10 and 30 hr. Total nitrogen remained constant, indirect evidence suggesting that protein was not used to supply oxidative energy The change‐over from carbohydrate to fat metabolism corresponded with observed changes in the respiratory quotient, and also with near‐completion of sporulation. Apparently carbohydrate provided the energy needed for the earlier stages of sporulation, whereas oxidation of lipids supported the teal stages as well as the metabolism during dormancy.