Cytochemistry of plasma membranes activated by phorbol myristate acetate and calcium ionophore in the alveolar and peritoneal macrophages of guinea pigs.

Moriguchi, Keiichi; Hirai, Kei-Ichi

doi:10.1267/ahc.19.465

Cited by 5 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4,5 and Table 1) (Rechardt & Hervonen, 1985). Therefore, no relationship was noted between the content of Ce and P in this study (Moriguchi & Hirai, 1986).…”

mentioning

confidence: 64%

Independent trafficking of flavocytochrome b558 and myeloperoxidase to phagosomes during phagocytosis visualised by energy‐filtering and energy‐dispersive spectroscopy‐scanning transmission electron microscopy

Moriguchi

2017

Journal of Microscopy

Self Cite

View full text Add to dashboard Cite

When polymorphonuclear leukocytes (PMNs) phagocytose opsonised zymosan particles (OPZ), free radicals and reactive oxygen species (ROS) are formed in the phagosomes. ROS production is mediated by NADPH oxidase (Nox), which transfers electrons in converting oxygen to superoxide (O ). Nox-generated O is rapidly converted to other ROS. Free radical-forming secretory vesicles containing the Nox redox center flavocytochrome b558, a membrane protein, and azurophil granules with packaged myeloperoxidase (MPO) have been described. Presuming the probable fusion of these vesicular and granular organelles with phagosomes, the translation process of the enzymes was investigated using energy-filtering and energy-dispersive spectroscopy-scanning transmission electron microscopy. In this work, the primary method for imaging cerium (Ce) ions demonstrated the localisation of H O generated by phagocytosing PMNs. The MPO activity of the same PMNs was continuously monitored using 0.1% 3,3'-diaminobenzidine-tetrahydrochloride (DAB) and 0.01% H O . A detailed view of these vesicular and granular structures was created by overlaying each electron micrograph with pseudocolors: blue for Ce and green for nitrogen (N).

show abstract

“…4,5 and Table 1) (Rechardt & Hervonen, 1985). Therefore, no relationship was noted between the content of Ce and P in this study (Moriguchi & Hirai, 1986).…”

mentioning

confidence: 64%

Independent trafficking of flavocytochrome b558 and myeloperoxidase to phagosomes during phagocytosis visualised by energy‐filtering and energy‐dispersive spectroscopy‐scanning transmission electron microscopy

Moriguchi

2017

Journal of Microscopy

Self Cite

View full text Add to dashboard Cite

show abstract

“…The original cerium method for H202 localization in phagocytes (Briggs et al 1975a), as well as subsequent studies on this topic (e.g., Briggs et al 1977;Moriguchi and Hirai 1986;Robinson and Batten 1990), required that the cytochemical incubation step be carried out prior to fixation. While sites of H202 production were readily detectable, there existed the possibility that reaction product could be internalized by endocytosis in living cells.…”

Section: H202 Detection: the Cerium Methodsmentioning

confidence: 99%

The NADPH oxidase complex of phagocytic leukocytes: a biochemical and cytochemical view

Robinson

Badwey

1995

Histochem Cell Biol

View full text Add to dashboard Cite

The NADPH oxidase complex catalyzes the formation of superoxide (O2.-) in phagocytic leukocytes. This paper reviews recent advances in our understanding of this enzyme system. Recent studies have defined conditions for reconstitution of this enzymatic activity with purified proteins in a cell-free system. The role of the individual proteins that make up the active complex, their regulation and the effects of mutations in these proteins are discussed. While these studies represent major achievements, it is clear from cytochemical investigations that additional levels of complexity exist in the modulation of the NADPH oxidase complex in vivo. A major role for cytochemical analysis in understanding the cell biological aspects of the generation of reactive oxygen species is discussed.

show abstract

“…as N-formyl-methionyl-leucyl-phenylalanine (11,12), concanavalin A and lectins (18), a two step stimulation mechanism (initial binding of stimulant to the membrane and secondary cell-cell adhesion) is needed for leukocytes to promote free radical production and that the NADPH oxidase, present at the unattached area of the plasma membrane, remains in the stand by state and is ready to produce radicals.…”

mentioning

confidence: 99%

Real time histochemistry of oxygen free radical production in human leukocytes.

Hirai

Moriguchi

Wang³

1992

Acta Histochem. Cytochem

Self Cite

View full text Add to dashboard Cite

Cytochemistry of plasma membranes activated by phorbol myristate acetate and calcium ionophore in the alveolar and peritoneal macrophages of guinea pigs.

Cited by 5 publications

References 18 publications

Independent trafficking of flavocytochrome b558 and myeloperoxidase to phagosomes during phagocytosis visualised by energy‐filtering and energy‐dispersive spectroscopy‐scanning transmission electron microscopy

Independent trafficking of flavocytochrome b558 and myeloperoxidase to phagosomes during phagocytosis visualised by energy‐filtering and energy‐dispersive spectroscopy‐scanning transmission electron microscopy

The NADPH oxidase complex of phagocytic leukocytes: a biochemical and cytochemical view

Real time histochemistry of oxygen free radical production in human leukocytes.

Contact Info

Product

Resources

About